The antifungal activity of ME-5CN05 and ME-blank systems was assessed using standard strains from the American Type Culture Collection (ATCC), namely Candida albicans ATCC 18804, Candida parapsilosis ATCC 22019 and Candida tropicalis ATCC 13803, provided by the Laboratory of Drug Development and Testing (LABDEM) belonging to the State University of Paraíba. Their activity was also assessed against Cryptococcus neoformans (LM10), provided by the Mycology Laboratory of the Federal University of Paraíba.
Candida parapsilosis atcc 22019
Manufactured by American Type Culture Collection
Sourced in United States
Candida parapsilosis ATCC 22019 is a reference strain of the yeast species Candida parapsilosis. It is a well-characterized isolate that is commonly used in laboratory research and testing.
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Lab products found in correlation
Candida albicans atcc 14053, by American Type Culture Collection (1 mentions)
E faecalis atcc 29212, by American Type Culture Collection (1 mentions)
E faecium atcc 700221, by American Type Culture Collection (1 mentions)
C albicans atcc 90028, by American Type Culture Collection (1 mentions)
S aureus atcc 29213, by American Type Culture Collection (1 mentions)
Candida krusei atcc 6258, by American Type Culture Collection (1 mentions)
4 protocols using candida parapsilosis atcc 22019
1
Antifungal Activity Assessment
2
Oral Candida Strains Collection
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Seven strains of oral Candida, which included Candida albicans ATCC 14053, Candida dubliniensis ATCC MYA-2975, Candida glabrata ATCC 90030, Candida krusei ATCC 14243, Candida lusitaniae ATCC 64125, Candida parapsilosis ATCC 22019 and Candida tropicalis ATCC 13803, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) for use in the study. Yeast peptone dextrose (YPD) broth (BD Difco, Sparks, MD, USA) was used to revive the cultures.
3
Antimicrobial Susceptibility Testing Strains
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S. aureus ATCC 29213, C. albicans ATCC 90028, E. faecalis ATCC 29212, E. faecium ATCC 700221, Candida krusei ATCC
6258, Candida parapsilosis ATCC 22019, Candida tropicalis ATCC 13803, Candida
glabrata ATCC MYA-2950, and A. fumigatus ATCC MYA-3626 were obtained from the American Type Culture Collection
(Manassas, VA). E. coli BW25113 (wild-type)
and JW5503-1 (tolC derivative of BW25113) were obtained
from the Coli Genetic Stock Center (New Haven, CT). S. aureus #1180973 (MRSA/MDR), A.
baumannii #1186154, C. albicans #1186858 (azole resistant), and C. albicans #1181444 (echinocandin resistant) were obtained from the JMI Laboratories
SENTRY strain collection.41 (link)P. aeruginosa (ΔmexAB-oprM::Cm ΔmexCD-oprJ::Gm ΔmexEF-oprN::ΩHg) PAM1626 is a derivative of PAO1 and was
the kind gift of O. Lomovskaya.36 (link)
6258, Candida parapsilosis ATCC 22019, Candida tropicalis ATCC 13803, Candida
glabrata ATCC MYA-2950, and A. fumigatus ATCC MYA-3626 were obtained from the American Type Culture Collection
(Manassas, VA). E. coli BW25113 (wild-type)
and JW5503-1 (tolC derivative of BW25113) were obtained
from the Coli Genetic Stock Center (New Haven, CT). S. aureus #1180973 (MRSA/MDR), A.
baumannii #1186154, C. albicans #1186858 (azole resistant), and C. albicans #1181444 (echinocandin resistant) were obtained from the JMI Laboratories
SENTRY strain collection.41 (link)P. aeruginosa (ΔmexAB-oprM::Cm ΔmexCD-oprJ::Gm ΔmexEF-oprN::ΩHg) PAM1626 is a derivative of PAO1 and was
the kind gift of O. Lomovskaya.36 (link)
4
Antifungal Susceptibility Testing Using Sensititre
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Sensititre YeastOne panels (Trek Diagnostic Systems, Thermo Scientific, East Grinstead, West Sussex, UK) were used for antifungal susceptibility testing. The plates contained serial twofold dilutions of amphotericin B (0.12 to 8 mg/L), 5-flucytosine (0.06 to 64 mg/L), fluconazole (0.12 to 256 mg/L), itraconazole (0.015 to 16 mg/L), voriconazole (0.008 to 8 mg/L), posaconazole (0.008 to 8 mg/L), anidulafungin (0.015 to 8 mg/L), micafungin (0.008 to 8 mg/L), and caspofungin (0.008 to 8 mg/L).
Antifungal susceptibility testing was performed by SYO according to the instructions provided by the manufacturer. Candida parapsilosis ATCC 22019 from the American Type Culture Collection (ATCC 22019) and Candida krusei ATCC 6258 were included as control strains in all the experiments. Minimum inhibitory concentrations (MICs) were determined after 24 h of incubation at 34–35 °C. The MIC was defined as the lowest concentration of antifungal agent at which the color in the well changed from red (positive, indicating growth) to blue (negative, indicating no growth).
Antifungal susceptibility testing was performed by SYO according to the instructions provided by the manufacturer. Candida parapsilosis ATCC 22019 from the American Type Culture Collection (ATCC 22019) and Candida krusei ATCC 6258 were included as control strains in all the experiments. Minimum inhibitory concentrations (MICs) were determined after 24 h of incubation at 34–35 °C. The MIC was defined as the lowest concentration of antifungal agent at which the color in the well changed from red (positive, indicating growth) to blue (negative, indicating no growth).
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