The human chondrosarcoma cell line (JJ012) was kindly supplied by Dr. Sean P. Scully’s laboratory at the University of Miami School of Medicine (Miami, FL, USA). SW1353 human chondrosarcoma cell line was purchased from the American Type Culture Collection (Manassas, VA, USA). Chondrosarcoma cell culture conditions were recorded as previously described40 (link). Human EPCs were isolated and cultured by a standard method as previously described41 ,42 (link). This study was approved by the Institutional Review Board of Mackay Medical College, New Taipei City, Taiwan (P1000002).
Sw1353 human chondrosarcoma cell line
Manufactured by American Type Culture Collection
Sourced in United States
The SW1353 human chondrosarcoma cell line is a well-characterized in vitro model derived from a human chondrosarcoma tumor. This cell line can be used for various research applications, including the study of chondrosarcoma biology and the development of potential therapies.
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2 protocols using sw1353 human chondrosarcoma cell line
1
Chondrosarcoma Cell Culture and EPC Isolation
2
Periostin Knockdown in Chondrosarcoma Cells
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Cell culture. The SW1353 human chondrosarcoma cell line was obtained from American Type Culture Collection and human lymphatic endothelial cells were obtained from Lonza (Walkersville, MD, USA). Each cell line was maintained according to the manufacturer's protocol. For the generation of periostin knockdown cells, periostin siRNA obtained from Thermo Fisher Scientific (Waltham, MA, USA) was used. Control siRNA was also obtained from Thermo Fisher Scientific. Cells were transiently transfected with control or periostin siRNA using Lipofectamine 3000 (Invitrogen, MA, USA). A total of 10 6 cells were plated with 10 ml of 5% FBS DMEM media and incubated for 72 h in 37˚C. Conditioned media (CM) were collected and cell particles were removed using centrifugation.
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