T47D (ATCC® HTB-133™), MCF7 (ATCC® HTB-22™), MDA-MB-231 (ATCC® CRM-HTB-26™), MDA-MB-468 (ATCC® HTB-132™) and BT-549 (ATCC® HTB-122™) human BC cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). T47D, MCF7, MDA-MB-231 and MDA-MB-468 cancer cells were grown in DMEM/Nutrient F-12 Ham (DMEM/F12) culture medium (Sigma) with 10% FBS (Sigma), penicillin (100 IU/mL) (Sigma) and streptomycin (100 μg/mL) (Sigma). BT-549 cells were maintained in Roswell Park Memorial Institute Medium (RPMI) culture medium (ATTC) with 0.023 U/mL insulin (Sigma), 10% FBS (ATTC), penicillin (100 IU/mL) (Sigma) and streptomycin (100 μg/mL) (Sigma). Mycoplasma-free cultures were maintained in a humidified atmosphere with 5% CO2 at 37°C.
Bt 549 atcc htb 122
Manufactured by American Type Culture Collection
Sourced in United States
The BT-549 (ATCC® HTB-122™) is a cell line derived from a human breast ductal carcinoma. It is routinely used in cell biology research, particularly in studies related to breast cancer.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Mcf 7, by Merck Group (1 mentions)
Mda mb 231, by Merck Group (1 mentions)
Mda mb 468, by Merck Group (1 mentions)
Dmem nutrient f 12 ham dmem f12 culture medium, by Merck Group (1 mentions)
T47d atcc htb 133, by American Type Culture Collection (1 mentions)
Atcc htb 22, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Mda mb 231 atcc htb 26, by American Type Culture Collection (1 mentions)
Hcc38, by American Type Culture Collection (1 mentions)
Hcc1937, by American Type Culture Collection (1 mentions)
3 protocols using bt 549 atcc htb 122
1
Culturing Human Breast Cancer Cell Lines
2
Breast Cell Line Culturing Protocol
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Immortalized human breast epithelial cell line MCF-10A and breast cancer cell lines MCF-7 (ATCC® HTB22™), MDA-MB-231 (ATCC® HTB26™), BT549 (ATCC® HTB122™), and BT474 (ATCC® HTB-20™) were obtained from the American Type Culture Collection (ATCC). The cells were cultured using medium described in a previous study [11 (link)]. All cells were cultured in humidified air containing 5% CO2 at 37°C.
3
Culturing Mouse Embryonic Fibroblasts and Triple-Negative Breast Cancer Cells
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Primary, p53KO and p53KO mouse embryonic fibroblasts transformed by HaRasV12 [35 (link)] were grown in DMEM-GlutaMax supplemented with 10% fetal bovine serum, 100 μg/ml streptomycin and 100 units/ml penicillin. SUM159 triple negative breast cancer cells were obtained from Asterand Bioscience, UK, and grown in Ham's F-12 medium supplemented with 5% fetal bovine serum, 10 μg/ml insulin, 1 μg/ml hydrocortisone, 100 μg/ml streptomycin and 100 units/ml penicillin. HCC1937 (ATCC-CRL-2336), HCC38 (ATCC-CRL-2314) and BT-549 (ATCC HTB-122) triple negative breast cancer cells were obtained from American Type Culture Collection. HCC1937 and HCC38 cells were grown in RPMI supplemented with 10% fetal bovine serum, 100 μg/ml streptomycin and 100 units/ml penicillin. BT-549 cells were grown in Dulbecco Modified Eagle Medium supplemented with 10% fetal bovine serum, 100 μg/ml streptomycin and 100 units/ml penicillin. All cell types were grown at 37°C in a humidified atmosphere containing 5% CO2 and were regularly checked for the absence of mycoplasma contamination.
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