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Pathhunter chinese hamster ovary k1 β arrestin2 human gpr6 express kits

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PathHunter™ Chinese hamster ovary (CHO)-K1 β-arrestin2 human GPR6 eXpress kits are a type of lab equipment used for cellular assays. The kits utilize a proprietary technology to measure the activation of the human G protein-coupled receptor 6 (GPR6) in CHO-K1 cells engineered to express the β-arrestin2 protein.

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Lab products found in correlation

Dichlorodimethylsilane for silanizing glass tubes, by Merck Group (2 mentions) Homogenous time resolved fluorescence camp hirange kits, by PerkinElmer (2 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) Cannabinoid ligands, by Cayman Chemical (1 mentions) L glutamine, by Avantor (1 mentions) Trypsin, by Avantor (1 mentions) Geneticin, by Avantor (1 mentions) Penicillin streptomycin, by Avantor (1 mentions)

2 protocols using pathhunter chinese hamster ovary k1 β arrestin2 human gpr6 express kits

1

GPCR Signaling Assays using HTRF and β-Arrestin

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Dulbecco's Modified Eagles's Medium (DMEM), penicillin/streptomycin, L-glutamine, trypsin, and geneticin were purchased from Mediatech (Manassas, VA). Fetal bovine serum was obtained from Atlanta Biologicals (Lawrenceville, GA). Dichlorodimethylsilane for silanizing glass tubes was purchased from Sigma-Aldrich (St. Louis, Mo). 384-well, round bottom, low volume white plates were purchased from Grenier Bio One (Monroe, NC). The homogenous time-resolved fluorescence (HTRF) cAMP Hirange kits were purchased from CisBio International (Bedford, MA). The PathHunter™ Chinese hamster ovary (CHO)-K1 β-arrestin2 human GPR6 eXpress kits were purchased from DiscoverX (Fremont, CA). Cannabinoid ligands were purchased from Cayman Chemical (Ann Arbor, MI).
Laun A.S., Shrader S.H, & Song Z.H. (2018). Novel inverse agonists for the orphan G protein-coupled receptor 6. Heliyon, 4(11), e00933.
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2

Measuring GPCR Activity in CHO Cells

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Dulbecco’s Modified Eagles’s Medium (DMEM), penicillin/streptomycin, l-glutamine, trypsin, and geneticin were obtained from VWR International (Radnor, PA). Fetal bovine serum was purchased from Atlanta Biologicals (Lawrenceville, GA) and dichlorodimethylsilane for silanizing glass tubes was purchased from Sigma-Aldrich (St. Louis, Mo). 384-well, round bottom, low volume white plates were purchased from Grenier Bio One (Monroe, NC). The PathHunter™ Chinese hamster ovary (CHO)–K1 β-arrestin2 human GPR6 eXpress kits were purchased from DiscoverX (Fremont, CA). The homogenous time-resolved fluorescence (HTRF) cAMP Hirange kits were purchased from CisBio International (Bedford, MA). N-acyl dopamines and other N-acylamidess were purchased from Cayman Chemical (Ann Arbor, MI).
Shrader S.H, & Song Z.H. (2019). Discovery of endogenous inverse agonists for G protein-coupled receptor 6. Biochemical and biophysical research communications, 522(4), 1041-1045.
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