Dii hdl

Manufactured by Thermo Fisher Scientific

Sourced in United States

The DiI-HDL is a fluorescently labeled high-density lipoprotein (HDL) product designed for cell and biomedical research applications. It is a ready-to-use solution that can be directly added to cell culture media or biological samples to monitor HDL uptake and trafficking.

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Lab products found in correlation

Hoechst, by Merck Group (1 mentions) Powerscan ht, by Sumitomo Pharma (1 mentions) Bodipy fl ldl, by Thermo Fisher Scientific (1 mentions) Dii ldl, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Dii ldl, by Thermo Fisher Scientific (1 mentions)

3 protocols using dii hdl

SR-BI Mutant Uptake Assay

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Huh7-siSR-BI cells were seeded in a 96-well plate at a density of 2 × 10⁴ cells/well and cultured for 24 h. Cells were transfected with the SR-BI WT and mutant S112F plasmids using Lipofectamine^TM. Complete culture medium was replaced 6 h after transfection and the cells cultured for 48 h before DiI-HDL (200 μg/mL, Alfa Aesar, United States) was added and the cells were cultured for an additional 4 h. Nuclei were stained with Hoechst (Merk, NJ, United States) for 10 min, and cells were observed and images captured using a microscope.

Gao R., Gao W., Xu G., Xu J, & Ren H. (2017). Single amino acid mutation of SR-BI decreases infectivity of hepatitis C virus derived from cell culture in a cell culture model. World Journal of Gastroenterology, 23(28), 5158-5166.

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Fluorescent Lipid Uptake Evaluation

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HDL and LDL labeled with 1,1’-Dioctadecyl-3,3,3,3’-tetramethylindocarbocyanine percholorate (DiI-HDL and DiI-LDL) were purchased from Alfa Aesar. Cells were washed with DMEM containing 0.5% fatty acid-free bovine serum albumin (Sigma) (medium A), and medium A containing 5μg DiI-HDL or DiI-LDL was added to each well. After incubation for 2 h at 37°C, cells were washed twice with PBS and observed with a FluoView FV1000 laser scanning confocal microscope. HDL uptake was determined by using HDL Uptake Assay Kit (Bio Vision) according to the manufacturer’s protocol. LDL uptake was determined after incubation with medium containing 10μg/ml BODIPY FL LDL (Thermo Fisher) without FCS for 24 h at 37°C by using PowerScanHT (DS Pharma Biomedical) according to the manufacturer’s protocol.

Yamamoto S., Fukuhara T., Ono C., Uemura K., Kawachi Y., Shiokawa M., Mori H., Wada M., Shima R., Okamoto T., Hiraga N., Suzuki R., Chayama K., Wakita T, & Matsuura Y. (2016). Lipoprotein Receptors Redundantly Participate in Entry of Hepatitis C Virus. PLoS Pathogens, 12(5), e1005610.

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DiI-HDL Cholesterol Uptake Assay

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HDL-derived cholesterol uptake was approximated using the fluorescent lipid, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), from labelled HDL particles (DiI-HDL, Alfa Aesar, Haverhill, MA) as modified from established techniques described in Supplementary Methods (32 (link)).

Gordon J.A., Noble J.W., Midha A., Derakhshan F., Wang G., Adomat H.H., Guns E.S., Lin Y.Y., Ren S., Collins C.C., Nelson P.S., Morrissey C., Wasan K.M, & Cox M.E. (2019). Upregulation of scavenger receptor B1 is required for steroidogenic and non-steroidogenic cholesterol metabolism in prostate cancer. Cancer research, 79(13), 3320-3331.

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