Au5400 automatic analyzer

All mice were euthanized using pentobarbital sodium after being fasted for 8 h; fasting 1 ml blood samples were obtained by subclavian venous puncture. Serum was obtained by centrifugation of blood at 3000 rpm for 15 min. Alanine aminotransferase (ALT) was measured as an indication of liver function by an automated hematology analyzer (Bayer, PA, USA). Serum glucose and lipid parameters including total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride (TG) were measured using Olympus AU5400 automatic analyzer in Shandong Provincial Hospital. Serum insulin levels were tested by mouse insulin ELISA Kit (CSB-E05071m).

The variables collected included demographics (age and gender), medical histories (hypertension, atrial fibrillation, diabetes, hyperlipidemia, and coronary heart diseases), the National Institute of Health Stroke Scale (NIHSS) score, the Alberta Stroke Program Early CT Score (ASPECTS), hemorrhagic transformation (30 (link)), reperfusion therapy method, and the Trial of Org 10172 in Acute Stroke Treatment (TOAST) classification (31 (link)). Successful recanalization after endovascular therapy was defined as a modified Thrombolysis in Cerebral Infarction grading system score of 2b or 3 (32 (link)). Blood samples were collected for laboratory measurements in the emergency department. White blood cell (WBC) counts, including absolute numbers of neutrophils were measured using an automated hematology analyzer (Sysmex, Kobe, Japan). Serum albumin levels were determined with an Olympus AU-5400 automatic analyzer (Olympus, Tokyo, Japan).