After relevant transfection, the apoptosis of TE1/DDP and KYSE410/DDP cells was evaluated through the Annexin V‐fluorescein isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection Kit (Beyotime). Briefly, transfected cells were harvested, washed, and resuspended. Then, 5 μl Annexin V‐FITC and 5 μl PI were added and maintained for 15 min in the dark to stain cells. The apoptotic cells were analyzed with a flow cytometry (BD Biosciences).
Fitc propidium iodide apoptosis detection kit
Manufactured by Beyotime
The FITC)/propidium iodide (PI) apoptosis detection kit is a laboratory tool used to identify and quantify apoptotic cells. It utilizes the fluorescent dyes FITC and PI to distinguish between viable, early apoptotic, and late apoptotic/necrotic cells. The kit provides a standardized method for the analysis of cell death and is commonly used in cell biology research.
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3 protocols using fitc propidium iodide apoptosis detection kit
1
Apoptosis Evaluation in Drug-Resistant Cells
2
Apoptosis Analysis of Transfected HCC Cells
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After treatment, 1 × 106 transfected HCC cells were measured using an annexin V-fluorescein isothiocyannate (FITC)/propidium iodide (PI) apoptosis detection kit (Beyotime) for 30 min at 37°C without light, following the manufacturer’s protocol. Then, apoptotic cells were quantified using a flow cytometer (BD Biosciences, USA) and analyzed using FlowJo analysis software.
3
Quantifying Apoptosis in Colorectal Cancer
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The apoptosis of HCT-116 and SW480 cells was detected using the Annexin V fluorescein isothiocynate (FITC)/propidium iodide (PI) apoptosis detection kit (Beyotime). The 6-well plates with transfected CRC cells were placed in a 37°C incubator for 48 h. Next, cells were harvested, FITC and PI were applied to stain the cells for 20 min in the absence of light, and the cells were analyzed by flow cytometry (FlowCam, Shanghai, China).
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