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Bgj398

Manufactured by Santa Cruz Biotechnology
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BGJ398 is a small molecule compound that functions as a selective fibroblast growth factor receptor (FGFR) inhibitor. It is designed to target and inhibit the activity of the FGFR family of receptor tyrosine kinases.

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Lab products found in correlation

Basic fibroblast growth factor (bfgf), by Merck Group (3 mentions) Crystal violet, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Mtt solution, by Merck Group (1 mentions) 24 well ultra low attachment plate, by Corning (1 mentions) Plrp6, by Cell Signaling Technology (1 mentions) Su5402, by Santa Cruz Biotechnology (1 mentions) P erbb2, by Cell Signaling Technology (1 mentions) Oct4a, by Cell Signaling Technology (1 mentions) P p70s6k, by Cell Signaling Technology (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) P egfr, by Cell Signaling Technology (1 mentions) P fgfr, by Cell Signaling Technology (1 mentions) Fgfr1, by Cell Signaling Technology (1 mentions) Erbb2, by Cell Signaling Technology (1 mentions) Fgfr2, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) P akt, by Cell Signaling Technology (1 mentions) P mtor, by Cell Signaling Technology (1 mentions) P 4ebp1, by Cell Signaling Technology (1 mentions)

5 protocols using bgj398

1

Sphere Assay for Stem Cells

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Cells were suspended in DMEM/F12 (1:1) supplemented with B27 supplement (Invitrigen), epidermal growth factor (EGF) and recombinant fibroblast growth factor basic (bFGF) (Sigma). EGF (20 ng/ml) and bFGF (25 ng/ml) were used together or separately depending on experimental designs. Cells were seeded in Ultra-Low attachment 24-well plates (Corning) at a density of 1,000 cells per well. In some experiments, 1 μM BGJ398 (Santa Cruz Biotechnology) or 0.1% DMSO was added. After 10-14 days, MTT solution (Sigma) was added to visualize any spheres formed and pictures were taken. The number of spheres was quantified by ImageJ 1.42q software (Wayne Rasband).
Hung J.S., Huang J., Lin Y.C., Huang M.J., Lee P.H., Lai H.S., Liang J.T, & Huang M.C. (2014). C1GALT1 overexpression promotes the invasive behavior of colon cancer cells through modifying O-glycosylation of FGFR2. Oncotarget, 5(8), 2096-2106.
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2

AZD4547 Receptor Signaling Profiling

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AZD4547 was purchased from LC Laboratories (Woburn, MA). FGFR1, FGFR2, p-FGFR, EGFR, p-EGFR, ErbB2, p-ErbB2, Akt, p-Akt, p-Erk1/2, mTOR, p-mTOR, 4EBP1, p-4EBP1, p70S6K, p-p70S6K, β-catenin, p-β-catenin, Dvl2, Oct4A, and p-Lrp6 primary antibodies were purchased from Cell Signaling (Danvers, MA). Erk2, p27, and β-actin primary antibodies, BGJ398, and SU5402 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Primary antibodies used for IF staining, including Cytokeratin 14 (K14), Cytokeratin 8/18 (K8/18), and α-smooth muscle actin (SMA) were purchased from Leica Biosystems (Buffalo Grove, IL), Developmental Studies Hybridoma Bank (DSHB; University of Iowa, Iowa City, IA), and Sigma (St. Louis, MO), respectively.
Zhao Q., Parris A.B., Howard E.W., Zhao M., Ma Z., Guo Z., Xing Y, & Yang X. (2017). FGFR inhibitor, AZD4547, impedes the stemness of mammary epithelial cells in the premalignant tissues of MMTV-ErbB2 transgenic mice. Scientific Reports, 7, 11306.
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3

Matrigel Invasion Assay for Cell Migration

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Cell invasion assays were performed in BioCoat Matrigel Invasion Chambers (Becton-Dickinson, Bedford, MA) according to the manufacturer's protocol. Briefly, 500 μl DMEM containing 10% FBS (GIBCO) or 25 ng/ml bFGF (Sigma) were added to the lower part of the chamber, whereas cells (8×104) in 500 μL DMEM were seeded to the upper part. Cells were allowed to invade the matrigel for 48 h. In some experiments, 1 μM BGJ398 (Santa Cruz Biotechnology) or 0.1% DMSO was included in the upper-chamber medium. Cells that invaded to the lower surface of the membrane were fixed and stained with 0.5% crystal violet (Sigma) and counted under a microscope in six random fields.
Hung J.S., Huang J., Lin Y.C., Huang M.J., Lee P.H., Lai H.S., Liang J.T, & Huang M.C. (2014). C1GALT1 overexpression promotes the invasive behavior of colon cancer cells through modifying O-glycosylation of FGFR2. Oncotarget, 5(8), 2096-2106.
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4

Integrated Signaling Pathways in Wound Healing

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Reagents were analytical grade and obtained from Sigma-Aldrich (UK) unless otherwise stated. Recombinant human FGF-2 (#100-18B) was from PeproTech (UK), recombinant human TGFβ1 (#240-B) from R&D Systems (UK), BGJ398 from Santa Cruz (USA), Gö6976 from Cell Signaling Technology (USA), and SB431542 from Sigma-Aldrich (UK). An equivalent volume of vehicle was used a control for each compound in experiments: 0.1% (w/v) bovine serum albumin (BSA) in 5 mM TRIS for FGF-2, dimethyl sulfoxide (DMSO) for BGJ398, Gö6976 and SB431542, and 0.1% (w/v) BSA in 4 mM HCl for TGFβ1. Antibodies to phosphorylated Smad2 (#3108), Smad2 (#5339), protein kinase Cα (PKCα, #2056), phosphorylated Akt (#4060), Akt (#9272), phosphorylated Erk1/2 (#4377), and Erk1/2 (#4695) were from Cell Signaling Technology (USA). Antibodies to syndecan-4 were from Santa Cruz (sc-12766) or Biovision, USA (#3644). Antibodies to FGF-2 (sc-79) were from Santa Cruz (USA), phosphorylated PKCα (07-790) from Merck Millipore (Germany), and β-actin (#A1978) from Sigma-Aldrich (UK).
Borland S.J., Morris T.G., Borland S.C., Morgan M.R., Francis S.E., Merry C.L, & Canfield A.E. (2017). Regulation of vascular smooth muscle cell calcification by syndecan-4/FGF-2/PKCα signalling and cross-talk with TGFβ. Cardiovascular Research, 113(13), 1639-1652.
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5

Boyden Chamber Cell Migration Assay

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Cells (8×104) in 500 μL DMEM containing 1% FBS were seeded into the upper part of the Boyden chamber with 8-μm pore filters (Corning, Cambridge, MA). Cell migration was induced by 10% FBS (GIBCO) or 25 ng/ml bFGF (Sigma) in the lower part. In some experiments, 1 μM BGJ398 (Santa Cruz Biotechnology) or 0.1% DMSO was included in the upper-chamber medium. After 48 h, cells that had migrated to the lower surface of the membrane were fixed and stained with 0.5% crystal violet (Sigma) and counted under a microscope in six random fields.
Hung J.S., Huang J., Lin Y.C., Huang M.J., Lee P.H., Lai H.S., Liang J.T, & Huang M.C. (2014). C1GALT1 overexpression promotes the invasive behavior of colon cancer cells through modifying O-glycosylation of FGFR2. Oncotarget, 5(8), 2096-2106.
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