pCMV-GFP (plasmid DNA; 3705 bp) was purchased from Altogen Biosystems (Las Vegas, NV, USA). Dasher GFP mRNA (1 kb) was purchased from Aldevron. SpCas9 and sgRNA targeting the TRAC or TRBC locus (TRAC: 5′-AGAGUCUCUCAGCUGGUACA-3′; TRBC: 5′-GGAGAATGACGAGTGGACCC-3′) were purchased from Integrated DNA Technologies (Coralville, IA, USA). Ribonucleoprotein (RNP) complexes were formed by mixing equimolar quantities of SpCas9 and each sgRNA and incubating for 10 min at room temperature before addition to cell suspensions in electroporation buffer.
Dasher gfp mrna
Manufactured by Aldevron
Dasher GFP mRNA is a laboratory reagent that produces green fluorescent protein (GFP) when translated in cells. The mRNA can be used to study gene expression and protein localization in various biological systems.
Automatically generated - may contain errors
2 protocols using dasher gfp mrna
1
CRISPR-Cas9 Ribonucleoprotein Transfection
2
Dasher GFP-mRNA Transfection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dasher GFP-mRNA was purchased from Aldevron, WI, aliquoted and stored at − 80 °C as per the recommendations. Lipofectamine MessengerMAX transfection reagent was obtained from Thermo Fisher Scientific, CA, stored at 4 °C.
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