Huh7-Lunet T7 cells were seeded onto glass coverslips. On the next day the cells were transfected with pTM-NS3-3′-based expression vectors by using the TransIT-LT1 transfection reagent (Mirus Bio). After 24 h the cells were washed three times with prewarmed PBS, fixed and processed for EM as described previously [24] (link). Specimens were examined with a Zeiss EM 10 transmission electron microscope at 60 kV.
Em 10 transmission
Manufactured by Zeiss
The EM 10 is a transmission electron microscope (TEM) designed and manufactured by Zeiss. It is a versatile instrument used for high-resolution imaging and analysis of nanoscale and atomic-scale structures. The EM 10 provides a core function of enabling the study of the internal structure and composition of a wide range of materials, including biological samples, metals, and ceramics.
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Lab products found in correlation
3 protocols using em 10 transmission
1
Electron Microscopy of Huh7-Lunet T7 Cells
2
Ultrastructural Analysis of Mouse Colon
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Mouse colon samples were fixed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer, then dehydrated, infiltrated with EPON-812 resin and embedded in capsules. The enclosed tissues were cut on a Reichert Ultracut 5 ultramicrotome in super thin sections. The sections was collected onto formvar-coated slot grid, and analyzed using a Zeiss EM-10 transmission electron microscope with digital acquisition.
3
Transmission Electron Microscopy Protocol
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Formalin-fixed paraffin-embedded tissues were deparaffinized with xylol, rehydrated, fixed with glutaraldehyde in cacodylate buffer (pH 7.2), and postfixed with 1% osmium tetroxide. Then, tissue was dehydrated via ethanol series, infiltrated with propylene oxide, and embedded in Epon resin. Ultrathin sections cut with ultramicrotome were stained with Toluidine blue, counter-stained with uranyl acetate, and examined using a Carl Zeiss EM10 transmission electron microscope.
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