The tissues or cells were collected and lysed using radio immunoprecipitation assay buffer (Beyotime Institute of Biotechnology, China) supplemented with PMSF (Beyotime Institute of Biotechnology). Equivalent protein quantities were subjected to SDS-PAGE and transferred to polyvinylidene fluoride membrane. Then, the blots were blocked with 5% fat free milk at room temperature and incubated overnight at 4℃ with the primary antibodies: anti-IL-9 (ab203386; abcom), anti-FOXO1 (AF603; Beyotime), anti-IRF4 (646402-1; biolegent), and anti-β-actin mouse mAb (AP0060; Bioworld). Blots were then washed and incubated with the appropriate secondary antibodies [goat anti-rabbit or goat anti-mouse (Bioworld)] for 1 h. Membranes were visualized with BeyoECL Plus (Beyotime).
Anti β actin mouse mab ap0060
Manufactured by Bioworld Technology
Anti-β-actin mouse mAb (AP0060) is a monoclonal antibody that recognizes the β-actin protein. It is a laboratory tool used for the detection and quantification of β-actin in various applications.
Automatically generated - may contain errors
2 protocols using anti β actin mouse mab ap0060
1
Protein Expression Analysis in Cells
2
Protein Expression Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The tissues or cells were collected and lysed using radio immunoprecipitation assay buffer (Beyotime Institute of Biotechnology, China) supplemented with PMSF (Beyotime Institute of Biotechnology). The protein concentration was quanti ed using a BCA kit. Equivalent protein quantities were subjected to SDS-PAGE and transferred to polyvinylidene uoride membrane. Then, the blots were blocked with 5% fat free milk at room temperature and incubated overnight at 4℃ with the primary antibodies: anti-IL-9 (ab203386; abcom), anti-FOXO1 (AF603; Beyotime), anti-IRF4 (646402-1;biolegent), and anti-β-actin mouse mAb (AP0060; Bioworld). Blots were then washed and incubated with the appropriate secondary antibodies [goat anti-rabbit or goat anti-mouse (Bioworld)] for 1 hour. Membranes were visualized with BeyoECL Plus (Beyotime).
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