Choline hydroxide (44% mass fraction, aqueous solution), glycine (Gly, ≥99%), l -alanine (Ala, ≥99%), serine (Ser, ≥99%), isoleucine (Ile,≥ 99%), aspartic acid (Asp, ≥99%), lysine (Lys, ≥99%), carbomer 940, and triethanolamine (>99%) were purchased from Aladdin and used as received. Ferulic acid (≥99%), and puerarin (≥99%) were purchased from Macklin and used as received. DMSO (≥99.9%), penicillin–streptomycin solution (100X), and l -glutamine (≥99%) were supplied by Aladdin. RPMI 1640 cell culture medium and fetal bovine serum (FBS) were purchased from Biological Industries. Phosphate buffered saline (PBS, pH = 7.4, 0.01 M) was purchased from Biorigin. CCK-8 was from Invigentech. A polyethersulfone (PES) membrane was supplied by Sartorius Stedim Biotech GmbH (Germany).
Ferulic acid
Manufactured by Maclin Biochemical Technology
Sourced in China
Ferulic acid is a naturally-occurring phenolic compound found in the cell walls of plants. It is a potent antioxidant and has been studied for its potential health benefits.
Automatically generated - may contain errors
Lab products found in correlation
Caffeic acid, by Maclin Biochemical Technology (3 mentions)
Protocatechuic acid, by Maclin Biochemical Technology (2 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Puerarin, by Maclin Biochemical Technology (1 mentions)
Rpmi 1640 cell culture medium, by Sartorius (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Vanillin, by Maclin Biochemical Technology (1 mentions)
Cinnamic acid, by Maclin Biochemical Technology (1 mentions)
Uv 8000, by Metash (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Eugenol, by Maclin Biochemical Technology (1 mentions)
Dimethylformamide dmf, by Merck Group (1 mentions)
K2co3, by Merck Group (1 mentions)
Q exactive focus, by Thermo Fisher Scientific (1 mentions)
1220 infinity 2 lc, by Agilent Technologies (1 mentions)
Butyric acid, by Maclin Biochemical Technology (1 mentions)
N hydroxysuccinimide, by Yuanye Bio-Technology (1 mentions)
7 protocols using ferulic acid
1
Choline-based Cell Culture Assay
2
Angelica sinensis Characterization and Compound Analysis
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The whole plant of mature Angelica sinensis (AS) was collected from Min Country, Gansu province of China in mid-October. The stem (without leaves) and root were separated into two parts, and air-dried at 50 °C for three days. The samples were ground in a knife mill to collect the saw dust passing through a 2 × 2 mm2 sieve. The powdered samples were sealed in a plastic bag and stored at 2−5 °C for further analysis.
Cinnamic acid (99%), vanillin (99%), p-hydroxybenzoic acid (98%), protocatechuic acid (97%), ferulic acid (98%), caffeic acid (98%) and campesterol (98%) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Z-ligustilide (98%), Butylidenephthalide (98%), Senkyunolide I (98%), stigmasterol (98%), and β-sitosterol (98%) were purchased from Chroma Biotechnol. Co., Ltd. (Chengdu, China). Coniferyl ferulate (97%) was synthesized in a laboratory according to the literature [36 (link)], and the structure and purity were elucidated by 1H NMR (Figure S1 ). The HPLC-grade acetonitrile (≥99.9%) and methanol (≥99.9%) were bought from Merck KGaA (Darmstadt, Germany).
Cinnamic acid (99%), vanillin (99%), p-hydroxybenzoic acid (98%), protocatechuic acid (97%), ferulic acid (98%), caffeic acid (98%) and campesterol (98%) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Z-ligustilide (98%), Butylidenephthalide (98%), Senkyunolide I (98%), stigmasterol (98%), and β-sitosterol (98%) were purchased from Chroma Biotechnol. Co., Ltd. (Chengdu, China). Coniferyl ferulate (97%) was synthesized in a laboratory according to the literature [36 (link)], and the structure and purity were elucidated by 1H NMR (
3
Synthesis and Characterization of Bioactive Compounds
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GA, N-(3-bromopropyl) benzene diamine, butyric acid, protocatechuic acid, benzoic acid, caffeic acid, ferulic acid, eugenol, and gallicin were obtained from Macklin (Shanghai, China). N-Hydroxy succinimide (NHS) and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were purchased from Yuanye (Shanghai, China). K2CO3, ethyl acetate, dimethylformamide (DMF), bovine serum albumin (BSA), and ovalbumin (OVA) were purchased from SIGMA. Ten 8-week SPF male mice were obtained from Vital River Laboratory Animal Technology (Beijing, China), license number SCXK (Beijing): 2021-0006; SP2/0 myeloma cells were purchased from Cell Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences.
HPLC was performed with 1220 infinityIILC (Agilent, Santa Clara, CA, USA). High-resolution mass spectrometry (HRMS) was carried out using Q Exactive Focus (Thermo Fisher, Waltham, MA, USA). UV spectrophotometer was recorded with UV-8000S (Metash, Shanghai, China). MALDI-TOF was recorded with Bruker Autoflex III (Bruker-Spectrospin AG, Karlsruhe, Germany).
HPLC was performed with 1220 infinityIILC (Agilent, Santa Clara, CA, USA). High-resolution mass spectrometry (HRMS) was carried out using Q Exactive Focus (Thermo Fisher, Waltham, MA, USA). UV spectrophotometer was recorded with UV-8000S (Metash, Shanghai, China). MALDI-TOF was recorded with Bruker Autoflex III (Bruker-Spectrospin AG, Karlsruhe, Germany).
4
Chitosan-Based Antioxidant Biomaterial
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Chitosan (CS) (deacetylated degree: 90%; average molecular weight: 1.5 × 105 Da), p-coumaric acid, caffeic acid, ferulic acid, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Fresh pork was purchased from a local market (Yangzhou, China).
5
Chitosan Functionalization with Ferulic Acid
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CS (deacetylated degree: 90%; average molecular weight: 1.5 × 105 Da), ferulic acid, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) and 1,1,3,3-tetramethoxypropane were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). All other reagents were of analytical grade.
6
Starch-based Enzymatic Modification
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Corn starch, α-amylase (AM, 50 U mg−1), amyloglucosidase (AMG, 100 U mL−1), were purchased from Shanghai Yuanye Biotechnology Co., Ltd (Shanghai, China). Ferulic acid (99%, HPLC grade), N,N′-carbonyldiimidazole (CDI), were purchased from Macklin Biochemical Technology Co. Ltd (Shanghai, China). All the reagents used were of analytical grade unless otherwise stated.
7
Standardized Phytochemical Characterization
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RYZB was purchased from Gansu Cheezheng Tibetan Medicine Co., Ltd., China, batch no. 2004007 (date of manufacture, April 2020; date of expiration, April 2023). Agarotetrol was obtained from Chengdu Push Biotechnology Co., Ltd., China, batch no. PU0295-0025, purity 99.7%. Piperine was obtained from Dalian meilunbio® Co., Ltd., China, batch no. PU0295-0025, purity 99.0%. Isoliquiritigenin was purchased from Shanghai Macklin Biochemical Co., Ltd., China, batch no. C10987045, purity 99.2%. Ferulic acid was purchased from Shanghai Macklin Biochemical Co., Ltd., China, batch no. C11347527, purity 99.2%. Vorinostat was obtained from Shanghai Macklin Biochemical Co., Ltd., China, batch no. C11120592, purity 98.7%. Methanol (chromatographic purity) was purchased from Merck, United States, batch no. l1108107033 and formic acid amine was from Sinopharm Chemical Reagents Co., Ltd., China, batch no. 30011661.
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