Isopsoralen (purity >98%, Fig. 1A ) and the MTT kit were purchased from Solebao Company (Beijing, China). Alpha-modified Eagle’s medium (α-MEM), fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Gibco (Rockville, MD, USA). TRIzol reagent was purchased from Tiangen (Beijing, China). The SYBR Green Master Mix was purchased from Imgenex (Littleton, CO, USA). Recombinant M-CSF and RANKL were obtained from R&D Systems (Minneapolis, MN, USA). The TRAP staining kit was purchased from Sigma Aldrich (St. Louis, MO, USA). Anti-P65, anti-phospho-P65, anti-IκB α, and anti-phospho-IκB α antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-MMP-9, anti-P50/P105, and anti-phospho-P50/P65 antibodies were purchased from Abcam (Cambridge, UK). Anti-GAPDH antibody was obtained from ABclonal Technology (Wuhan, Hubei, China). Anti-NFATc1 antibody was obtained from AiFang Biological (Changsha, Hunan, China). The anti-CTSK antibody was obtained from Proteintech Group (Wuhan, Hubei, China).
Anti ctsk antibody
Manufactured by Proteintech
Sourced in China
The Anti-CTSK antibody is a primary antibody that specifically recognizes the Cathepsin K (CTSK) protein. CTSK is a protease enzyme involved in bone and cartilage degradation. This antibody can be used to detect and study the CTSK protein in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Anti p65, by Cell Signaling Technology (1 mentions)
Alpha modified eagle s medium α mem, by Thermo Fisher Scientific (1 mentions)
Recombinant m csf, by R&D Systems (1 mentions)
Trap staining kit, by Merck Group (1 mentions)
Anti phospho p65, by Cell Signaling Technology (1 mentions)
Anti phospho iκbα, by Cell Signaling Technology (1 mentions)
Anti gapdh antibody, by ABclonal (1 mentions)
Anti iκbα, by Cell Signaling Technology (1 mentions)
Trizol reagent, by Tiangen Biotech (1 mentions)
Rankl, by R&D Systems (1 mentions)
Anti mmp9, by Abcam (1 mentions)
Staurosporine, by MedChemExpress (1 mentions)
Rankl 462 tec, by R&D Systems (1 mentions)
M csf 416 ml, by R&D Systems (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Solarbio (1 mentions)
Mito tracker green, by Beyotime (1 mentions)
Lyso tracker red, by Beyotime (1 mentions)
2 protocols using anti ctsk antibody
1
Isopsoralen Modulates Osteoclastogenesis
2
Osteoclast Differentiation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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M-CSF (416-ML) and RANKL (462-TEC) were purchased from R&D Systems. Staurosporine was purchased from MedChemExpress (HY-15141). MitoTracker Green (C1048), LysoTracker Red (C1046), enhanced mitochondrial membrane potential assay kit with JC-1 (C2003S), ATP assay kit (S0026) and a ROS assay kit (S0033S) were purchased from Beyotime. An Annexin V-FITC/PI apoptosis detection kit and cells genomic DNA extraction kit were purchased from Solarbio (CA1020). Following anti-bodies were used in our experiments: anti-Fbxo7 antibody (GeneTex #GTX65829), anti-Bpgm antibody (Santa Cruz #sc-373819), anti-TOM antibody (Proteintech #11802-1-AP), anti-SQSTM1/p62 antibody (Affinity #AF5384), anti-BNIP3 antibody (Affinity #DF8188), anti-PINK1 antibody (Affinity #DF7742), anti-LC3B antibody (Affinity #AF4650), anti-APG5L/ATG5 antibody (Affinity #DF6010), anti-DC-STAMP antibody (ABclonal #A14630), anti-CTSK antibody (Proteintech #11239-1-AP), anti-NFATc1 antibody (CST #8032),anti-TRAP antibody (Abcam #ab191406).
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