Blood specimens were collected from 16‐week‐old mice after overnight fasting. The serum levels of fasting triglyceride (TG), total cholesterol (TC), high‐density lipoprotein (HDL), low‐density lipoprotein (LDL) and non‐esterified fatty acids (NEFA) were measured using a biochemical analyser (TBA120FR; Toshiba, Tokyo, Japan). The serum level of fasting insulin was quantified using a mouse insulin enzyme‐linked immunosorbent assay (Crystal Chem, Downers Grove, IL, USA). The fasting glucose level was measured using a biochemical analyser (TBA120FR; Toshiba). After euthanization, livers and free gonadal adipose tissues were harvested and measured to calculate the liver‐to‐weight ratio and gonadal fat‐to‐weight ratio.
Tba 120fr
Manufactured by Toshiba
Sourced in Japan, United States
The TBA-120FR is a laboratory centrifuge designed for general-purpose applications. It features a maximum speed of 12,000 rpm and a maximum relative centrifugal force of 20,400 g. The unit is equipped with a brushless motor and a microprocessor-controlled digital display for setting and monitoring the operating parameters.
Automatically generated - may contain errors
Lab products found in correlation
Advia 2120i, by Siemens (6 mentions)
Edta 2k, by BD (3 mentions)
Elisa assay kit, by Shanghai Enzyme-linked (3 mentions)
Rta00, by R&D Systems (2 mentions)
Xt 2000iv, by Sysmex (2 mentions)
Adams a1c ha 8160, by Arkray (2 mentions)
Acl elite pro, by Werfen (2 mentions)
9nc sodium citrate, by BD (2 mentions)
Immunite 2000xpi, by Siemens (2 mentions)
Synergy ht, by Agilent Technologies (2 mentions)
Osteolinks dpd kit, by Sumitomo Pharma (2 mentions)
Osteocalcin rat elisa system, by GE Healthcare (2 mentions)
Hlc 723 g7 hplc system, by Tosoh (1 mentions)
Automatic biochemistry radiometer au640, by Olympus (1 mentions)
Edta k2tubes, by BD (1 mentions)
Advia centaur, by Siemens (1 mentions)
Immulite 2000 xpi, by Siemens (1 mentions)
Protein quantitative assay kit, by Thermo Fisher Scientific (1 mentions)
Mouse insulin enzyme linked immunosorbent assay elisa kit, by Crystal Chem (1 mentions)
Micro 17r, by Hanil (1 mentions)
99 protocols using tba 120fr
1
Metabolic Profiling in Fasted Mice
2
Biochemical Profile of Fasting Participants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Venous blood samples were collected from all participants, as part of their routine monitoring procedure on a day independent of the gait analysis, after overnight fasting, and analyzed on the hospital premises, using automatic biochemical analyzers, under standard conditions. The white blood cell count (WBC), and concentrations of blood glucose (GL), serum total cholesterol, triglycerides (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), high-sensitivity C reactive protein (hsCRP) and B12 were measured by an automatic analyzer (Toshiba TBA 120FR; Toshiba Medical Systems Co., Ltd., Tokyo, Japan).
3
Gestational Diabetes Cohort Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We conducted a prospective cohort study, which recruited all the pregnant women having visited the obstetric clinic for prenatal care at National Taiwan University hospital obstetric clinic from November 2013 to April 2018. The pregnant women with overt diabetes, defined as diabetes diagnosed before pregnancy or at the first prenatal visit, and those with twin or multiple pregnancies, were excluded. The medical history, findings from physical examination, and results of laboratory tests of the participants were recorded at the first prenatal visit. All participants underwent a 75g oral glucose tolerance test (OGTT) at 24th–28th gestational weeks to diagnose gestational diabetes. Body mass index (BMI) was calculated by body weight in kilograms divided by the square of body height in meters. Plasma glucose, hemoglobin A1c (HbA1c), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), plasma triglyceride (TG) and C-peptide were measured with an autonomic analyzer (Toshiba TBA 120 FR, Toshiba Medical Systems Co., Ltd., Tokyo, Japan). Written informed consent was obtained from each patient before enrollment in the cohort. This study was reviewed and approved by the Institutional Review Board of National Taiwan University Hospital.
4
Oral Glucose Tolerance Test Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A standard 75-g OGTT was performed after a fast at least 8 hours. Blood samples were collected and stored at -80°C for serological assay. Normal glucose tolerance (fasting plasma glucose [FPG] < 5.56 mmol/L and 2-h plasma glucose [PG] < 7.8 mmol/L), impaired fasting glucose (IFG) (FPG = 5.56–6.9 mmol/L), impaired glucose tolerance (2-h PG = 7.8–11.0 mmol/L), and diabetes mellitus (FPG ≥ 7 mmol/L and/or 2-h PG ≥ 11.1 mmol/L) were defined according to the ADA diagnostic criteria [32 (link)]. GA was measured using the LUCICA GA-L kit (Asahi Kasei Pharma, Tokyo, Japan) and the Beckman Coulter AU2700 Chemistry Analyzer (Beckman Coulter Systems Co., Nyon, Switzerland). GA in paired serum and plasma samples from twelve subjects were measured to determine whether the method of sample collection affected GA measurements. Plasma glucose and HbA1c were measured using automatic analyzers (Toshiba TBA 120FR, Toshiba Medical Systems Co., Tokyo, Japan and HLC-723 G7 HPLC systems, Tosoh Corporation, Tokyo, Japan, respectively). The HbA1c assay was certified by the National Glycohemoglobin Standardization Program [33 (link)] and standardized to the DCCT reference assay.
5
Serum Biomarkers and Liver XOD Activity in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An aliquot (200 µL) of serum samples of mice from different groups was used for measurement of serum uric acid and blood urea nitrogen. Their concentrations were determined by automatic biochemical analyzer (TOSHIBA TBA-120FR, Toshiba Medical Systems Co., Tochigi, Japan) according to manufacturer’s operation instructions. Proinflammatory cytokines (e.g., IL-1β and TNF-α) in serum samples of mice from different groups were measured by using the Mouse ELISA Commercial Kit (Jianglai Biotechnology Co., Ltd., Shanghai, China). The concentrations were calculated based on the standard curves.
In addition, XOD activity in hepatic tissue was also determined. Briefly, the mouse liver samples were pulverized into fine powder with a stainless-steel mortar and pestle at the temperature of liquid nitrogen. A powder sample from each liver tissue was further homogenized (50 mg per 1.0 buffer solution) and centrifuged (12,000 rpm, 10 min, 4°C) to obtain the supernatant. Then these supernatant samples were used for determination of XOD activity by following the manufacturer’s instructions (Jiangcheng, Nanjing, China).
In addition, XOD activity in hepatic tissue was also determined. Briefly, the mouse liver samples were pulverized into fine powder with a stainless-steel mortar and pestle at the temperature of liquid nitrogen. A powder sample from each liver tissue was further homogenized (50 mg per 1.0 buffer solution) and centrifuged (12,000 rpm, 10 min, 4°C) to obtain the supernatant. Then these supernatant samples were used for determination of XOD activity by following the manufacturer’s instructions (Jiangcheng, Nanjing, China).
6
Biochemical Analysis of Serum Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A biochemical analyzer (TBA120FR, Toshiba Medical Systems Corporation, Japan) was used to detect many biochemical indexes such as blood urea nitrogen (BUN), glucose (GLU), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total protein (TP), immunoglobulin M (IgM), immunoglobulin A (IgA) and immunoglobulin G (IgG) in serum. The kit for this experiment was provided by Nanjing Jiancheng Biological Engineering Research Institute (Nanjing Jiancheng, Nanjing, China).
7
Biochemical Profiling of Serum Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum biochemical parameters, including blood glucose (GLUC), total protein (TP), triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), albumin (ALB), alkaline phosphatase (ALP), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine (CRE), were measured using spectrophotometric kits following the manufacturer's instructions (TBA-120FR, Toshiba Medical Systems Corporation, Japan) and determined using an Automatic Biochemistry Radiometer (Au640, Olympus).
8
Serum Biochemical Profile Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum samples were analyzed for levels of total protein (TP, g/L), albumin (ALB, g/L), globulin (GLO, g/L), cholesterol (CHOL, mmol/L), triglyceride (TG, mmol/L), high-density lipoprotein (HDL, mmol/L), low-density lipoprotein (LDL, mmol/L), aspartate aminotransferase (AST, U/L) and alanine aminotransferase (ALT, U/L) on an automatic biochemical analyzer (TBA-120FR, Toshiba Co., Ltd., Tokyo, Japan) using Shanghai Kehua biochemical diagnosis kits (Shanghai Kehua Bio-Engineering Co., Ltd., Shanghai, China).
9
Hematological and Biochemical Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was withdrawn from the auricular artery of all rabbits and collected in EDTA‐K2 tubes (BD, East Rutherford, NJ, USA) containing an anticoagulant at the prediet stage (week 0) and then at 3‐week intervals after commencing the HFD. RBC, hemoglobin, and platelets were measured using a hematology analyzer (ADVIA2120i; Siemens, Munich, Germany). Plasma was separated by centrifugation at 2000 g for 10 min at 4 °C. Total cholesterol (TCHOL), low‐density lipoprotein (LDL), high‐density lipoprotein (HDL), phospholipid, blood urea nitrogen (BUN), and creatinine concentrations, as well as aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, were measured using an automated biochemistry analyzer (TBA 120FR, Toshiba, Tokyo, Japan).
10
Serum Biochemistry Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For serum biochemistry analysis, the collected blood samples were centrifuged at 3000 g for 15~20 min for serum isolation. Levels of a total of 20 markers were tested including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine (CRE), glucose (GLU), total cholesterol (CHO), total protein (TP), high density lipoprotein (HDL), low density lipoprotein (LDL), albumin (ALB), total bilirubin (TBIL), triglyceride (TG), calcium (Ca), inorganic phosphorus (IP), sodium (Na), potassium (K), chloride (Cl), phospholipid (PL) and high-sensitive C-reactive protein (hsCRP). The analysis was measured by automated biochemistry analyzer (TBA-120FR, Toshiba, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!