Standard Western-blot assays were used as described previously59 (link). The following primary antibodies were used: anti-PPARα (Abcam, Cata# ab24509), anti-ACLY (Santa Cruz, Cata# sc-517267), anti-FASN (Santa Cruz, Cata# sc-48357), anti-ACSL1 (cell signaling, Cata# 4047 S), anti-ACSL5 (Santa Cruz, Cata# sc-365478), anti-STAT3 (Santa Cruz, Cata# sc-8019), anti-pSTAT3 (cell signaling, Cata# 9145 S), anti-STAT1 (cell signaling, Cata# 14994 S), anti-pSTAT1 (cell signaling, Cata# 9177 S), anti-STAT4 (cell signaling, Cata# 2653 S), anti-pSTAT4 (cell signaling, Cata# 4134 S), anti-AKT (Santa Cruz, Cata# sc-5298, 1:2000 dilution), anti-pAKT (cell signaling, Cata# 9018 S), anti-pERK (cell signaling, Cata# 4376), anti-ERK (cell signaling, Cata# 9102), anti-pMAPK (cell signaling, Cata# 4511), anti-MAPK (cell signaling, Cata# 9212) and anti-β-actin (Sigma, Cata# A5441, 1:100,000 dilution) antibodies. Other than anti-AKT and anti-β-actin antibodies, the dilution for all other antibodies was 1:1000.
Anti acly
Manufactured by Santa Cruz Biotechnology
Anti-ACLY is a laboratory product offered by Santa Cruz Biotechnology. It is an antibody that specifically targets the Acetyl-CoA Carboxylase Lyase (ACLY) protein. ACLY is an enzyme involved in cellular metabolism. The primary function of Anti-ACLY is to facilitate the detection and study of ACLY in various research and experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti stat3, by Santa Cruz Biotechnology (2 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti p erk, by Cell Signaling Technology (1 mentions)
Anti akt, by Santa Cruz Biotechnology (1 mentions)
Anti pparα, by Abcam (1 mentions)
Anti fasn, by Santa Cruz Biotechnology (1 mentions)
Anti stat4, by Santa Cruz Biotechnology (1 mentions)
Anti pstat4, by Santa Cruz Biotechnology (1 mentions)
Anti pmapk, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti p stat3, by Cell Signaling Technology (1 mentions)
Anti pparα, by Thermo Fisher Scientific (1 mentions)
Anti scd1, by Cell Signaling Technology (1 mentions)
Fast cast tgx stain free, by Bio-Rad (1 mentions)
Miniprotean tgx stain free gels, by Bio-Rad (1 mentions)
Trans blot system, by Bio-Rad (1 mentions)
Image lab, by Bio-Rad (1 mentions)
3 protocols using anti acly
1
Western Blot Antibody Characterization
2
Immunohistochemistry of FFPE Tissue Sections
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Formalin-fixed and paraffin-embedded (FFPE) muscle and WAT tissue sections were stained with hematoxylin and eosin as described previously58 (link). IHC staining of FFPE liver tissue sections were performed as described previously59 (link). Briefly, tissue sections were deparaffinized in xylene and rehydrated in ethanol and water, followed by antigen retrieval by boiling slides in antigen unmasking solution (Cata#: h3300, Vector Laboratories) for 10 min. The following primary antibodies were used: anti-pSTAT3 (cell signaling, Cata# 9145 S), anti-STAT3 (Santa Cruz, Cata# sc-8019), anti-PPARα (Thermo Fisher Scientific, Cata# MA5-37652) and anti-ACLY (Santa Cruz, Cata# sc-517267). The dilution for all antibodies was 1:10.
3
Quantification of SGBS Protein Expression
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SGBS protein expression was quantified by western blotting. Cells were lysed in RIPA buffer (15 mM Tris-HCl, pH 7.4, 1% NP40 1%, 1.25% sodium deoxycholate 1.25%, 150 mM NaCl, 1 mM EDTA, 1% SDS). Equal amounts of protein were loaded on 10% or 4-15% sodium dodecyl sulfate polyacrylamide gels (Fast Cast TGX Stain-Free or 4-15% Mini-Protean TGX Stain-Free gels, BioRad, Hercules, CA). Blotting was done on PVDF membranes using a BioRad Transblot system. Membranes were blocked and probed overnight with anti-SCD1 (Cell Signaling 2438S), anti-AdipoQ (Sigma A6354), anti-14-3-3γ (Proteintech 12381-1-AP), anti-ACLY (Santa Cruz Biotechnology SC-517267) or anti-(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted July 2, 2020. ; https://doi.org/10.1101/2020.06.30.180083 doi: bioRxiv preprint 9 ASAH1 (Santa Cruz Biotechnology SC-136275) in 5% milk in TBS with 0.5% Tween-20.
Proteins were detected with enhanced chemiluminescence (ECL; Thermo Scientific, Waltham, MA). Image Lab (BioRad) was used to quantify the corresponding protein band intensities normalized to total protein intensity.
The copyright holder for this preprint this version posted July 2, 2020. ; https://doi.org/10.1101/2020.06.30.180083 doi: bioRxiv preprint 9 ASAH1 (Santa Cruz Biotechnology SC-136275) in 5% milk in TBS with 0.5% Tween-20.
Proteins were detected with enhanced chemiluminescence (ECL; Thermo Scientific, Waltham, MA). Image Lab (BioRad) was used to quantify the corresponding protein band intensities normalized to total protein intensity.
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