In the cabinet drying process, the mushroom samples were washed, cut into thin slices, and dried at 50°C till the constant weight was obtained by grinding using a mixer grinder (Philips, India) in a similar way as in the case of a freeze dryer (as shown in Figures 1 –4 ). The dried mushroom samples were then sealed in plastic pouches and then stored in frozen conditions for further analysis. Sample preparation was carried out using the different formulations (Table 1 ).
Mixer grinder
Manufactured by Philips
Sourced in India
The Philips Mixer Grinder is a kitchen appliance designed for grinding, mixing, and blending a variety of ingredients. It features a powerful motor and multiple speed settings to accommodate different task requirements.
Automatically generated - may contain errors
Lab products found in correlation
8 protocols using mixer grinder
1
Mushroom Drying and Preservation Protocol
2
Preparation of Areca Seed Powder
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Areca seeds were sourced from an agricultural area in Sringeri, Chikmagalur District, Karnataka, India. The plant material was initially washed with distilled water several times and dried in shade for 2–3 days. The obtained dried seeds were powdered into uniform sized particles using an electric mixer (Philips Mixer Grinder, 750 W) and were stored at room temperature until further use.
3
Cytotoxic Mushroom Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For cytotoxic study of mushroom fruit body, aqueous cold extraction was done. In this extraction, 20 g of air-dried pieces of mushroom were powdered in Mixer Grinder (Philips HL1606/03 500 W, HP, India) and dissolved in 100 mL of distilled water followed by incubation in orbital shaker (Metrex, New Delhi, India) for 24 h at 25 ± 2 °C. This aqueous mixture of mushroom centrifuged at 5000 rpm for 15 min at 4 °C, supernatant was collected and filter by Whatman filter paper No. 1 (125 mm). The filtrate kept on rotatory evaporator (BUCHI, B-300, Mumbai, India) for the evaporation of water until the extract is appear like semisolid gel. This final extract was stored at 4 °C until further use.
4
Freeze-Drying of Button Mushrooms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Button mushrooms (Agaricus bisporus) were selected, washed, and cut into thin slices to provide uniform mixing with carrier agents. The samples were mixed with different carrier agents at different concentrations by adding 25 ml of distilled water to formulate the paste for proper mixing for drying purposes. In freeze-drying, mushroom slices were frozen at a temperature of −80°C in a conventional freezer and then subjected to freeze-drying in a freeze-dryer (SP Scientific VirTis Sentry 2.0, USA) at a pressure of 5 mTorr (0.666 Pa) for a period of 38 h. The obtained flakes were ground using a Mixer Grinder (Philips, India) (as shown in Figures 1 –4 ).
5
Protein Extraction from Defatted Flours
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seeds from all the three different varieties were first manually cleaned for removing unwanted foreign constituents. Milling of grains was done using a grinder (Phillips, Mixer Grinder, New Delhi, India) at ambient temperature to obtain fine flour. The flour samples were then sieved using a sieve (60 mesh-British standards). The flour was further defatted and decolorized using methanol:chloroform (60:30). The slurry was then centrifuged (Eppendorf, 5810R, Hamburg, Germany) at 3000 × g for 10 min and the obtained pellet was air dried. The defatted and decolorized flour was used for the extraction of protein using the previously followed method of Jhan et al., 2021 [9] . Briefly, slurry of flour was prepared at the ratio of 1(flour): 4 (double distilled water) w/v. The pH of slurry was maintained at 9.5 using sodium hydroxide (1 N) and kept at stirring for one hour at room temperature which enhanced the protein solubility. The slurry was then centrifuged at 4000 × g for 15 min. The pH of supernatant obtained was then adjusted to 4 using hydrochloric acid (1 N) and kept undisturbed for about one hour. The centrifugation was again carried out for 15 min at 4000 × g and pellet recovered was required protein concentrate. The purity of the extracted protein was found to be 76.9%, 82.87% and 86.65% using kjeldhal method.
6
Extraction and Characterization of trans-Ethyl para-Methoxycinnamate from Kaempferia galanga Rhizome
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
K. galanga rhizome (KGR) was grounded coarsely in a mixer grinder (Philips, India). Powdered KGR (250 g) was hydro distilled in Clevenger apparatus at 100–105 °C for about 6 h. The condensed hydrodistillate separated and stored at −4 °C until further analysis using GC and GC/MS following the method [14 ]. The trans-ethyl para methoxycinnamate (EPMC) was separated and purified from oil by crystallization method. Compound identification and their structure elucidation done by various spectroscopic techniques such as 1H-NMR, IR, COSY HMQC, and HMBC. The spectral characteristics matched with the reported data [16 (link)].
7
Ethanolic Extract of A. sanguinolenta
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole plant of A. sanguinolenta was collected (from medicinal garden of Ramkrishna Mission Ashram, Narendrapur, West Bengal during the month of December), authenticated (by Botanical Survey of India, Howrah, West Bengal-711103), washed, shade dried, chopped, powdered (mixer grinder -Philips HL), stored, labeled, and refrigerated (at 4°C). Hot continuous extraction of coarse powder was done with 300 ml ethanol (Mercks) by Soxhlet apparatus (Borosil). Wet plant extract (20.5462 g) by weight thus obtained, were concentrated by distillation using a rotary evaporator (RE100PR0MFGD silicogex/USA Takashi) to get dry plant extract (12.5462 g) by weight. Dry extract (1000 μg) was then mixed with 1 ml of DMSO (Mercks) to prepare the 0.2% solution of whole plant ethanolic extract of A. sanguinolenta. It was ten times of MIC of A. sanguinolenta against S. mutans and L. acidophilus which was determined in a previous study by the present authors.
8
Stem Extraction and Lyophilization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The stem samples were washed with tap water followed by deionized water to remove soil and other traces. These were dried in the air for 4 weeks. The air-dried stems were chopped and further converted into fine small pieces by mixer grinder (Philips, India). Dried stems (10 g) was extracted overnight with deionized water (Direct-Q, Millipore) (1:1 w/v) in orbital shaker at 37 °C and 180 rpm to yield the thick juice. Extracts were then centrifuged at 15,000 g for 10 min at 4 °C. The extraction was repeated three times for each sample and the supernatant was collected. The percolate from three repeats of each sample was then concentrated in a rotary vacuum evaporator at 50–60 °C. The supernatant juice was quick-freezed at −80 °C (Thermo-Fisher, Germany) and lyophilized (Freezone 4.5 Labconco, CA, USA) to yield a dry homogenous powder (0.3 g) and stored at −80 °C. The lyophilized powder from various samples was reconstituted in LC-MS grade water to make a solution of 5.0 mg/ml. Solutions were vortexed and centrifuged at 15,000 g for 20 minutes at 4 °C temperature. The supernatants were carefully removed and filtered through 0.22 µm syringe filters and transferred to 96 well plates. The complete workflow of the study design is depected in Fig. S1 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!