Specimens were prepared according to the methodology given in the immunocytochemical staining section above. The primary antibodies (anti-mouse cyclin A monoclonal antibody (clone 6E6, 1:15; Abcam) and anti-rabbit Ki-67 polyclonal antibody (1:100; Abcam) were applied, incubated for 90 min at room temperature, and washed. Then, the cells on the slides were incubated with FITC-labeled anti-mouse IgG and tetramethylrhodamine (TRITC)-labeled anti-rabbit IgG (1:40; Abcam) for 30 min at room temperature. Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Immediately after treatment, the cells were observed under a fluorescence microscope (Bz-x700; Keyence Corporation, Tokyo, Japan).
Fitc labeled anti mouse igg
Manufactured by Abcam
Sourced in United States, United Kingdom
FITC-labeled anti-mouse IgG is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is conjugated with the fluorescent dye FITC (Fluorescein isothiocyanate). This antibody can be used to detect and visualize mouse IgG in various immunoassays and imaging applications.
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Lab products found in correlation
3 protocols using fitc labeled anti mouse igg
1
Dual Immunofluorescence Staining Protocol
2
Quantifying Donor-Specific Antibodies by Flow Cytometry
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Levels of circulating donor-specific antibodies (DSA; IgG and IgM) in recipient sera at the indicated time points were assessed by flow cytometry, as previously described (20 (link)). Briefly, recipient sera were incubated with C57BL/6 donor splenocytes at 37°C for 30 min, after which washed cells were incubated with fluorescein isothiocyanate (FITC)-labeled anti-mouse IgG (Abcam, ab6724, 1:100) and rhodamine red-conjugated anti-mouse IgM (Jackson ImmunoResearch Laboratories, 115-297-020, 1:100) at 4°C for 1 h. Cells were analyzed by FACScan (Becton–Dickinson, Lincoln Park, NJ, United States) flow cytometry with results expressed as mean fluorescence intensity to reflect individual serum DSA levels.
3
Donor-Specific Antibody Detection
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Circulating donor-specific IgG and IgM antibodies were assessed in recipient sera by flow cytometry. In short, recipient sera were incubated with C3H donor splenocytes at 37°C for 30 min, and washed cells were then incubated with FITC-labeled anti-mouse IgG (Abcam, Cambridge, England) and rhodamine red-conjugated anti-mouse IgM (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) at 4°C for 1 h. Cells were analyzed by flow cytometry with results expressed as mean fluorescence intensity to reflect individual serum antidonor antibody levels.
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