Anti-Mff (17090-1-AP; ProteinTech) was used at 1:1,000 dilution for Western blot and 1:500 dilution for immunofluorescence. Anti-Fis1 (10956-1-AP; ProteinTech) was used at 1:1,000 for Western blot and 1:500 for immunofluorescence. Anti-Tubulin (DM1-α; Sigma-Aldrich) was used at 1:10,000 dilution for Western blot. Drp1 was detected using a rabbit monoclonal antibody (D6C7; Cell Signaling Technologies) at 1:500 dilution for immunofluorescence. Anti-INF2 rabbit polyclonal was described previously (Ramabhadran et al., 2011 (link)). Organelle marker antibodies for Western blot included anti-ATP synthase mouse monoclonal (A21351 ; Molecular Probes), anti-Sec63 (ARP46839 ; Aviva), and anti-Pmp70 rabbit polyclonal (4200181; Sigma-Aldrich), all used at 1:1,000.
Anti fis1 10956 1 ap
Manufactured by Proteintech
Sourced in United States
Anti-Fis1 (10956-1-AP) is a primary antibody that recognizes the Fis1 protein. Fis1 is a mitochondrial protein that plays a role in mitochondrial fission. This antibody can be used for applications such as western blotting and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anti mff 17090 1 ap, by Proteintech (1 mentions)
Anti tubulin dm1α, by Merck Group (1 mentions)
A21351, by Thermo Fisher Scientific (1 mentions)
Ab184247, by Abcam (1 mentions)
Ab157457, by Abcam (1 mentions)
Ab193216, by Abcam (1 mentions)
Ab205236, by Abcam (1 mentions)
Ab221661, by Abcam (1 mentions)
Anti c ebp homologous protein chop, by Cell Signaling Technology (1 mentions)
Anti p drp 1 ser616, by Cell Signaling Technology (1 mentions)
Ab8227, by Abcam (1 mentions)
2 protocols using anti fis1 10956 1 ap
1
Antibody Dilutions for Western Blot and Immunofluorescence
2
Mitochondrial Dynamics Protein Expression
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Protein expression was measured using western blotting. Briefly, the immunoblots were probed with anti-mfn1 (ab221661, Abcam), anti-mfn2 (ab205236, Abcam), anti-OPA1 (ab157457, Abcam), anti-Drp1 (ab184247, Abcam), anti-p-Drp1 (Ser637) (ab193216, Abcam), anti-p-Drp1 (ser616) (#3455, Cell Signaling Technology), anti-fis1 (10956-1-AP, Proteintech), anti-C/EBP homologous protein (CHOP) (#2895, Cell Signaling Technology), anti-myosin light chain (MLC) (#3672, Cell Signaling Technology), anti-p-MLC (#3675, Cell Signaling Technology), or anti-β-actin (ab8227, Abcam) overnight at 4° C followed by incubation with the corresponding secondary antibodies at room temperature for 1 h. The blots were visualized with ECL-plus reagent (SignalFire, USA).
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