NK cell phenotype of melanoma patients enrolled in the trial was examined using fluorochrome-conjugated antibodies against the following cell-surface markers: CD56-FITC, CD3-PC7, CD16-APC, CD69-BV421, NKp30-BV711, CXCR3-BV421, CCR3-BV510 (BD Biosciences; San Diego, CA), NKp44-PerCP eFluor 710 (eBioscience; San Diego, CA), CXCR1-PE (R&D Systems; Minneapolis, MN), CCR7-BV711 (BioLegend; San Diego, CA), and matching IgG isotype controls from the same vendors. The immune checkpoint and NK cell activation receptor panel included the following markers: Zombie NIR Fixable Viability Dye (BioLegend; San Diego, CA), CD3-PE-Vio770 (Miltenyi Biotec; San Diego, CA), ANK-1-PE (Santa Cruz Biotechnology; Dallas, TX), TIGIT-PerCP eFluor 710 (eBioscience), CD45-BUV395, CD56-BV510, CD16-BUV737, NKG2D-APC, NKp46-BV711, CD69-BV421, and PD-1-BV650 (BD Biosciences).
Nkp44 percp efluor 710
Manufactured by Thermo Fisher Scientific
NKp44-PerCP eFluor 710 is a fluorescently labeled antibody that can be used for the detection and analysis of NKp44-expressing cells in flow cytometry experiments. The PerCP eFluor 710 fluorochrome is conjugated to the NKp44 antibody, providing a specific signal for the identification of NKp44-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Cd16 apc, by BD (2 mentions)
Cd56 fitc, by BD (2 mentions)
Nkp30 bv711, by BD (2 mentions)
Cxcr3 bv421, by BD (2 mentions)
Cxcr1 pe, by R&D Systems (2 mentions)
Cd3 pe vio770, by Miltenyi Biotec (2 mentions)
Cd56 bv510, by BD (2 mentions)
Cd16 buv737, by BD (2 mentions)
Cd69 bv421, by BD (2 mentions)
Nkg2d apc, by BD (1 mentions)
Tigit percp efluor710, by Thermo Fisher Scientific (1 mentions)
Cd3 pc7, by BD (1 mentions)
Ccr7 bv711, by BioLegend (1 mentions)
Zombie nir fixable viability dye, by BioLegend (1 mentions)
Pd 1 bv650, by BD (1 mentions)
Cd3 pe cy7, by BD (1 mentions)
Cd45 buv395, by BD (1 mentions)
Zombie nir, by BioLegend (1 mentions)
Ccr7 bv510, by BD (1 mentions)
2 protocols using nkp44 percp efluor 710
1
Comprehensive NK Cell Profiling in Melanoma
2
Multiparametric Characterization of NK Cells
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One-step staining of cell-surface antigens was performed using fluorochrome-conjugated primary antibodies as previously described (10 (link), 18 (link), 33 (link)). For the analysis of blood NK cells two antibody panels were constructed around CD56-FITC, CD16-APC, and CD3-PE-Cy7 (BD Bioscience, San Jose, CA) antibodies. NK cell activation receptors were evaluated with CD69-BV421, NKp30-BV711 (BD Bioscience), and NKp44-PerCP eFluor 710 (eBioscience, San Diego, CA) antibodies. Chemokine expression levels were tested using CXCR1-PE (R&D Systems, Minneapolis, MN), CXCR3-BV421, and CCR7-BV510 (BD Bioscience) antibodies. For the TINK analysis, Zombie NIR (BioLegend), CD45 BUV395, CD56 BV510, CD16 BUV737 (BD Bioscience), and CD3 PE-Vio770 (Miltenyi Biotec) antibodies were used. Suitable IgG controls were acquired from the same vendors. FACS analyses were performed using the BD LSRFortessa™ cell analyzer, and analyzed using FlowJo v10 (FlowJo, LLC; Ashland, OR) software.
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