HeLa, A549, and THP-1 cell lines were purchased from ATCC, the HaCat cell line was purchased from Cell Lines Service, HUVECs and NHEKs were purchased from PromoCell, and Caco-2 cells were purchased from the Riken Cell Bank. HeLa and A549 cells were maintained in Dulbecco’s modified Eagle’s medium (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS; Gibco) and 50 μg/ml gentamicin (Nacalai Tesque), and the THP-1 cells were cultured in RPMI 1640 medium (Nacalai Tesque) supplemented with 10% FBS and 50 μg/ml gentamicin. THP-1 cells were differentiated into macrophages by stimulating them with 50 ng/ml phorbol 12-myristate for 72 h. HUVECs were maintained with the endothelial cell growth medium 2 kit (PromoCell) supplemented with 10% FBS and 50 μg/ml gentamicin. NHEKs were cultured with the keratinocyte growth medium 2 kit (PromoCell), and Caco-2 cells were maintained in minimum essential medium (Wako) supplemented with 10% FBS and 50 μg/ml gentamicin. Cells were incubated in a 5% CO2 incubator at 37°C.
Minimum essential medium (mem)
Manufactured by Fujifilm
Sourced in Japan, United States
The MEM is a laboratory equipment product manufactured by Fujifilm. It is designed for use in various scientific and research applications. The core function of the MEM is to provide a controlled environment for cell and tissue culture experiments.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Streptomycin, by Fujifilm (5 mentions)
Fetal bovine serum (fbs), by Merck Group (4 mentions)
Penicillin, by Fujifilm (4 mentions)
Rpmi 1640 medium, by Fujifilm (4 mentions)
Dulbecco modified eagle medium (dmem), by Fujifilm (4 mentions)
Dmem high glucose, by Fujifilm (3 mentions)
Acetyltrypsin, by Merck Group (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Mem non essential amino acids, by Fujifilm (2 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions)
Dmem nutrient mixture f 12 ham, by Fujifilm (2 mentions)
Vero cells, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Bovine serum albumin fraction 5, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Fujifilm (2 mentions)
Non essential amino acids (neaa), by Fujifilm (2 mentions)
Gentamicin, by Fujifilm (1 mentions)
Hacat cell line, by Cytion (1 mentions)
Caco 2 cells, by RIKEN Cell Bank (1 mentions)
26 protocols using minimum essential medium (mem)
1
Cell Culture Maintenance Protocols
2
Efficient Neuronal Differentiation of P19 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To induce neuronal differentiation, the stably transfected P19 cells were cultured in bacteriological-grade Petri dishes at a seeding density of 1 × 105 cells/ml in the presence of 1 μM all-trans-retinoic acid (Sigma-Aldrich, USA) in minimum essential medium (Wako, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, USA)38 (link). After 4-day culture, cells were dissociated into single cell by 0.05% trypsin and were replanted in a poly-L-lysine coated tissue-culture dish at a density of 3 × 105 cells/ml in an N2 serum-free medium (DMEM/F12 supplemented with 5 mg/mL insulin, 50 mg/ml human transferrin, 20 nM progesterone, 60 mM putresine, and 30 nM sodium selenite). The cells were then maintained for at most 6 days with replacement of medium every 2 days.
3
HTLV-1 Infection of Retinal Pigment Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used the ARPE-19 human retinal pigment epithelial cell line (American Type Culture Collection, Manassas, VA) as ocular cells, and the MT-2 cell line as an HTLV-1-infected T-cell line. We used Jurkat cells as a control T-cell line. MT2 and Jurkat cells were cultured in RPMI 1640 (Wako Pure Chemical Corporation, Osaka, Japan) supplemented with 10% fetal bovine serum (FBS) (GE Healthcare Japan, Tokyo, Japan) and 1% penicillin/streptomycin. ARPE-19 cells were cultured in minimum essential medium (Wako Pure Chemical Corporation) with the same supplements. All cell lines were incubated in a humidified incubator at 37°C under an atmosphere of 5% CO2. In vitro infection by HTLV-1 was performed using the standard co-culture method (Akagi et al., 1986 (link); Graziano et al., 1987 (link); Liu et al., 2006 (link)). Briefly, ARPE-19 cells were plated and co-cultured with three times the number of MT2 or Jurkat cells at 48 h using cell culture inserts (Thermo Fisher Scientific, Waltham, MA). 1.5 × 105 ARPE-19 cells were used in cytometric bead assay (CBA) and annexin V assay. 2 × 104 ARPE-19 cells were used in cell counts, TNF receptor analysis, and the measurement of HTLV-1 proviral load.
4
Murine Cancer Cell Lines and Macrophages
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A murine colon cancer cell line Colon26 (aka CT26) [23 (link),67 (link)] and a murine calvaria-derived osteoblastic cell line MC3T3-E1 [68 (link)] were obtained from Cell Bank at RIKEN BioResource Research Center (Tsukuba, Japan). A rapidly metastatic subline, LuM1, was generated from Colon26 as described previously [23 (link),35 (link),36 (link),42 (link),43 (link)]. MMP3-knockout LuM1 (MMP3-KO) cells were generated using the genome-editing method as described below. A murine macrophage-like cell line RAW-D (a subline of RAW264.7) was kindly provided by Prof. Toshio Kukita (Kyushu University, Fukuoka, Japan) [69 (link)]. Colon26, LuM1, MMP3-KO LuM1, and MC3T3-E1 were maintained in RPMI1640 with 10% fetal bovine serum (FBS) and penicillin, streptomycin, and amphotericin B. RAW-D cells were cultured in minimum essential medium (MEM; Wako Pure Chemicals, Osaka, Japan) containing 10% FBS, supplemented with penicillin (100 U/mL) and streptomycin (100 mg/mL).
5
Cell Line Origin, Culture, and Consent
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following cell lines (Kasumi-1, NB4, NALM-6, Jurkat, K562, Daudi, IM-9, RPMI8226, NCI-H929, HeLa, and RK13) were obtained from ATCC between 2004 and 2013. FL-218 was obtained from the Japanese Collection of Research Bioresources Cell Bank in 2011. Normal human skin fibroblasts (NB1RGB) were obtained from the RIKEN BioResource Center in 2014. All cell lines were passaged for less than 6 months. No further authentication was done for these cell lines in the past 6 months. Bone marrow samples were collected from patients admitted to the Japanese Red Cross Medical Center between February 2014 and December 2014; written informed consent was obtained from all patients prior to collection. All relevant study-related protocols were approved by the Institutional Review Board of the Japanese Red Cross Medical Center and the Institute of Medical Science, The University of Tokyo. Cells were grown in appropriate culture medium supplemented with 10% fetal bovine serum (JRH Biosciences), 100 U/mL penicillin (Wako), and 100 μg/mL streptomycin (Wako). Cell lines derived from hematological malignancies and patient bone marrow mononuclear cells were grown in RPMI 1640 medium (Wako). HeLa cells and NB1RGB cells were grown in minimum essential medium (MEM), alpha modification (Wako). RK13 cells were grown in standard MEM (Wako).
6
Establishment and Maintenance of Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human cervical carcinoma HeLa (RCB0007), hepatoma HepG2 (RCB1648), colon carcinoma Caco-2 (RCB0988), embryonic kidney 293T (RCB2202), and mouse myoblast C2C12 (RCB0987) cells were obtained from Riken Cell Bank (Tsukuba, Japan). Human embryonic kidney 293 (JCRB9068), rat hepatoma Fao (89042701), and rat cardiomyocyte H9c2 (CRL-1446) cells were obtained from Japanese Collection of Research Bioresources (JCRB) Cell Bank (National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan), ECACC (Salisbury, UK), and ATCC (Manassas, VA, USA), respectively. These cells were maintained in Eagle’s minimum essential medium (MEM, FUJIFILM Wako Pure Chemical Corporation) containing antibiotics and 10% fetal bovine serum, except for C2C12 and H9c2 cells, which were maintained in Dulbecco’s modified Eagle medium (FUJIFILM Wako Pure Chemical Corporation). Primary cultured rat cardiomyocytes (CMC02) were obtained from COSMO BIO (Tokyo, Japan).
7
MDCK Cell Culture in MEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Madin-Darby canine kidney (MDCK) cells were grown in Eagle’s minimum essential medium (MEM; FUJIFILM Wako Pure Chemical Industries, Ltd., Osaka, Japan) containing 7% fetal bovine serum (FBS; Biowest SAS, Nuaillé, France) and 1% antibiotics (penicillin and streptomycin; FUJIFILM Wako).
8
Culturing HepG2 Cells for Biochemical Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HepG2 cells (RCB1648) purchased from Riken Cell Bank (Tsukuba, Japan) were maintained in Minimum Essential Medium Eagle (MEM, FUJIFILM Wako) containing 10% fetal bovine serum (FBS, Thermo Fisher Scientific Inc., Waltham, MA, USA), 1% MEM nonessential amino acids (FUJIFILM Wako), 100 units/mL penicillin G, and 100 μg/mL streptomycin (FUJIFILM Wako) at 37 °C under a humidified atmosphere containing 5% CO2. Each sample was prepared in DMSO to achieve a final concentration of 0.5%.
9
Characterization of Mouse Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The FM3A cell line derived from C3H mouse breast cancer cells was purchased from the JCRB Cell Bank (Ibaraki, Osaka, Japan) and cultured in MEM (Wako, Tokyo, Japan). 3T3 cells derived from BALB/c mouse fibroblast cells were purchased from the RIKEN Cell Bank (Tsukuba, Ibaraki, Japan) and cultured in DMEM (Sigma Aldrich, St. Louis, MO, USA). The MTT060562 cell line derived from C3H mouse breast cancer cells and T-Ag-MOSE cell line derived from C3H mouse ovarian cancer cells were purchased from the JCRB Cell Bank (Ibaraki, Oosaka, Japan) and cultured in DMEM (Sigma Aldrich). The radiation-induced myeloid leukemia cell line from C3H mice, 8047, was established at the National Institute of Radiological Sciences in Chiba [29 (link)]. The cells were cultured in RPMI-1640 medium (Sigma Aldrich). The data of T-Ag-MOSE and 8047 cell lines were shown in Supplementary Figures. Medium was supplemented with 10% fetal calf serum, penicillin (50 units/mL; Invitrogen, Carlsbad, CA, USA), and streptomycin (50 μg/mL; Invitrogen). The cells were cultured at 37°C in a humidified atmosphere of 5% CO2.
10
Cell Culture Conditions for Neuroblastoma, Kidney, and Monkey Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SH-SY5Y cells derived from human neuroblastoma were grown in Dulbecco’s modified Eagle medium (DMEM):Nutrient Mixture F-12 (Wako, Osaka, Japan) supplemented with 10% fetal bovine serum (FBS), 100 IU/mL penicillin, and 100 μg/mL streptomycin at 37°C with 5% CO2. Vero cells derived from African green monkey kidney epithelial cells were grown in Eagle’s minimum essential medium (MEM) (Wako), supplemented with 10% FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin at 37°C with 5% CO2. Human embryonic kidney 293T (293T) cells were cultured in high-glucose DMEM (Wako) supplemented with 10% FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin at 37°C with 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!