Rosa26creer

The Otub1-flox mice (in B6 genetic background) were generated using embryos obtained from The European Conditional Mouse Mutagenesis Program (EUCOMM, strain Otub1^{tm1a(EUCOMM)Hmgu}). Otub1-flox mice were crossed with CD4^Cre transgenic mice (both in B6 genetic background and from Jackson Laboratories) to produce age-matched Otub1^+/+CD4^Cre (named WT) and Otub1^fl/flCD4^Cre (named T cell-conditional Otub1 knockout or TKO) mice. The Otub1-flox mice were also crossed with ROSA26-CreER (Jackson Laboratories) to generate Otub1^+/+CreER and Otub1^fl/flCre-ER mice, which were then injected i.p. with tamoxifen (2 mg per mouse) in corn oil daily for four consecutive days to induce Cre function for generation of WT and induced Otub1 KO (iKO) mice. OT-I and Pmel1 TCR–transgenic mice, B6.SJL (CD45.1⁺), C57BL/6, Rag1-KO, and Il15ra-KO mice were from Jackson Laboratory. Experiments were performed with young adult (6–8 weeks) female and male mice except where indicated otherwise. All mice were in B6 genetic background and maintained in a specific pathogen–free facility of The University of Texas MD Anderson Cancer Center, and all animal experiments were done in accordance with protocols approved by the Institutional Animal Care and Use Committee of the University of Texas MD Anderson Cancer Center.