Biospin magnet

Mice were anesthetized and positioned in a cradle. A 30‐cm horizontal bore Bruker Biospin magnet operating at 7 T (Bruker BioSpin GmbH) was used. A BA6 gradient set and mouse head coil were used to perform all MRI experiments as previously described.12 All animals were imaged every 2‐3 days until the end of the study starting at 10 days post‐G55 implantation surgery.

Nude mice were anesthetized and positioned in a stereotaxic cradle. A 30-cm horizontal bore Bruker Biospin magnet operating at 7 Tesla (T; Bruker BioSpin GmbH, Karlsruhe, Germany), was used with a S116 gradient set to perform all MRI experiments. An EPI (echo planar imaging) transceiver ¹H 50W coil with a 38.0 mm inner diameter was used for signal transmission and detection. Multiple-slice, multiple echo (MSME) imaging (FOV = 2.50 x 2.50 cm², TR = 2000 ms, TE = 17.5 or 58.2 ms, matrix = 192, averages = 2, slices = 16, slice thickness = 1 mm) was used to calculate tumor volumes and to inspect tumor morphology. Multi slice spin echo T₁-weighted images (TR = 1000.0 ms, TE = 14 ms, FOV = 2.50 × 2.50 cm², averages = 2, slices = 16, matrix size = 256 × 256) were also performed and acquired before and 15 min after intravenous contrast agent injection (Gd-DTPA, Magnevist, Bayer Inc., Wayne, NY, USA; 0.4mmol/kg).