Mouse tissue was cryopreserved using O.C.T. and cut into 5 µm sections. Cryosections were fixed in ice‐cold methanol/acetone. After blocking in 10% goat serum with 0.1% Triton X‐100, sections were incubated with primary antibodies diluted in 10% goat serum at 4°C overnight. Following primary antibodies were used: anti‐CD31 (550274, BD Biosciences, 1:50), anti‐cleaved caspase 3 (9664, Cell Signaling Technology, 1:100), anti‐ColIV (2150‐1470, Bio‐Rad, 1:250), anti‐ERG (ab93513, Abcam, 1:1,000), anti‐α‐SMA (C6198, Sigma‐Aldrich, 1:200), anti‐Ki‐67 (14‐5698‐82, Thermo Fisher Scientific, 1:100) or FITC‐Isolectin B4 (L2895, Sigma‐Aldrich, 1:100). DAPI and fluorophore‐conjugated secondary antibodies were added before embedding the sections in fluorescent mounting medium (DAKO). Images were acquired using a confocal microscope (Zeiss LSM 700) and analysis was done using Fiji software.
Ab93513
Manufactured by Abcam
Sourced in United Kingdom
Ab93513 is a lab equipment product offered by Abcam. It is a tool designed for use in research laboratories, but no further details about its core function or intended use are available.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 700, by Zeiss (2 mentions)
Accuri c6, by BD (2 mentions)
Fluorescent mounting medium, by Agilent Technologies (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti ki67, by Thermo Fisher Scientific (1 mentions)
Anti coliv, by Bio-Rad (1 mentions)
Fitc isolectin b4, by Merck Group (1 mentions)
Anti α sma, by Merck Group (1 mentions)
Anti cd31, by BD (1 mentions)
2 protocols using ab93513
1
Cryosection Immunofluorescence Assay
2
Quantifying Integrin αvβ3 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To determine the integrin αvβ3 expression levels in different cell lines, a flow cytometry assay was performed. Briefly, cells were collected and washed with phosphate-buffered saline (PBS) containing 1% bovine serum albumin (BSA). After blocking with 5% BSA in PBS, cells were incubated with an anti-CD51 + CD61 antibody [23C6], (1:20, ab93513; Abcam, Cambridge, UK) for 30 minutes at 4°C. After washing with PBS containing 1% BSA, the cells were re-suspended in PBS, and were then analyzed using Accuri™ C6 (BD, Franklin Lakes, NJ, USA) with the 488 nm excitation wavelength and the 530 nm emission wavelength. The fluorescein isothiocyanate (FITC) signal intensity was analyzed using the Accuri™ C6 software (BD).
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