Nitrite content in cell culture supernatant was measured to indirectly reflect the content of NO using Griess reagent.48 (link)–50 (link) Briefly, NO release from HUVECs was detected by enzymatic conversion of nitrate to nitrite by the enzyme nitrate reductase, followed by the Griess reaction to form a colored azo dye product per manufacturers protocols (Enzo, ADI-917–020). HUVECs transfected with miR-615–5p mimic or non-specific miRNA controls were cultured for 60 hours in 12-well plates and stimulated with 50ng/ml VEGF for 1 or 3 hrs as indicated. Supernatant from ECs were collected to detect total NO release.
Adi 917 020
Manufactured by Enzo Life Sciences
Sourced in United States
The ADI-917-020 is a laboratory equipment product offered by Enzo Life Sciences. It serves as a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using adi 917 020
1
Quantification of Nitric Oxide Release
2
Biomarker Quantification in Sleep Study
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Blood samples were taken at the end of the ten-minute supine rest after the sleep study. Samples were subjected to a routine laboratory procedure to have the serum separated. ET-1, CRP, IL-6, TNF-α, and VEGF were measured with Enzyme-Linked Immunosorbent Assay kits (ET-1, ADI-900-202A, Enzo Life Sciences Inc., New York, USA; CRP, 88-7502, eBioscience, Inc., California, USA; IL-6, BMS213HS, Bender MedSystems GmbH, Vienna, Austria; TNF-α, BMS223HS, Bender MedSystems GmbH, Vienna, Austria; VEGF, BMS277/2, Bender MedSystems GmbH, Vienna, Austria). The metabolites of NO were measured by the Griess reaction using prepared kits (ADI-917-020, Enzo Life Sciences Inc., New York, USA) before which samples were ultrafiltered through a 10,000 MWCO filter (Amicon® Ultra-15 10K, Merck Millipore Ltd., County Cork, Ireland) to remove protein as recommended.
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