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Universal mycoplasma test kit

Manufactured by American Type Culture Collection
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The Universal Mycoplasma Test Kit is designed for the detection of mycoplasma contamination in cell cultures. The kit uses a PCR-based method to identify the presence of mycoplasma species. It provides a reliable and efficient way to assess the mycoplasma status of cell lines.

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Lab products found in correlation

Effectene transfection reagent, by Qiagen (2 mentions) Crl 2539, by American Type Culture Collection (2 mentions) Crl 2638, by American Type Culture Collection (2 mentions)

2 protocols using universal mycoplasma test kit

1

Generating Gp96-Ig Expressing Tumor Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine B16-F10 melanoma (CRL-6475), 4T1 metastatic mammary carcinoma (CRL-2539), and CT26 colon carcinoma (CRL-2638) cell lines were purchased from the American Type Culture Collection (ATCC), cultured according to manufacturer’s directions and tested for mycoplasma using the Universal Mycoplasma test kit (ATCC). For vaccination studies, CT26 mouse colon carcinoma cells used for transfection were maintained in Iscove’s Modified Dulbecco’s Media (IMDM) supplemented with glutamine and 10% Fetal Bovine Serum. The CT26-gp96-Ig (ImPACT, Heat Biologics, North Carolina) cell line was generated by transfecting CT26 cells with a DNA plasmid expressing murine gp96-Ig using Effectene transfection reagents (Qiagen Hilden, Germany) according to manufacturer’s directions. Transfectants were selected with G418 and a single cell clone secreting a high-level of gp96-Ig was isolated by limiting dilution. Gp96-Ig levels were determined by ELISA. All cells were cultured at 37°C with 5% CO2.
Triplett T.A., Garrison K.C., Marshall N., Donkor M., Blazeck J., Lamb C., Qerqez A., Dekker J.D., Tano Y., W.e.i.-.C.h.e.n.g.-.L.u., Karamitros C.S., Ford K., Tan B., Zhang M., McGovern K., Coma S., Kumada Y., Yamany M.S., Sentandreu E., Fromm G., Tiziani S., Schreiber T.H., Manfredi M., Ehrlich L.I., Stone E, & Georgiou G. (2018). Reversal of IDO-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nature biotechnology, 36(8), 758-764.
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2

Generating Gp96-Ig Expressing Tumor Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine B16-F10 melanoma (CRL-6475), 4T1 metastatic mammary carcinoma (CRL-2539), and CT26 colon carcinoma (CRL-2638) cell lines were purchased from the American Type Culture Collection (ATCC), cultured according to manufacturer’s directions and tested for mycoplasma using the Universal Mycoplasma test kit (ATCC). For vaccination studies, CT26 mouse colon carcinoma cells used for transfection were maintained in Iscove’s Modified Dulbecco’s Media (IMDM) supplemented with glutamine and 10% Fetal Bovine Serum. The CT26-gp96-Ig (ImPACT, Heat Biologics, North Carolina) cell line was generated by transfecting CT26 cells with a DNA plasmid expressing murine gp96-Ig using Effectene transfection reagents (Qiagen Hilden, Germany) according to manufacturer’s directions. Transfectants were selected with G418 and a single cell clone secreting a high-level of gp96-Ig was isolated by limiting dilution. Gp96-Ig levels were determined by ELISA. All cells were cultured at 37°C with 5% CO2.
Triplett T.A., Garrison K.C., Marshall N., Donkor M., Blazeck J., Lamb C., Qerqez A., Dekker J.D., Tano Y., W.e.i.-.C.h.e.n.g.-.L.u., Karamitros C.S., Ford K., Tan B., Zhang M., McGovern K., Coma S., Kumada Y., Yamany M.S., Sentandreu E., Fromm G., Tiziani S., Schreiber T.H., Manfredi M., Ehrlich L.I., Stone E, & Georgiou G. (2018). Reversal of IDO-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nature biotechnology, 36(8), 758-764.
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