Live dead 488 fixable green dead cell stain kit

Manufactured by Thermo Fisher Scientific

The LIVE/DEAD 488 Fixable Green Dead Cell Stain Kit is a fluorescent dye-based solution designed to detect dead cells in a sample. The kit utilizes a cell-impermeant dye that binds to proteins within dead cells, producing a bright fluorescent signal. This stain can be used in flow cytometry applications to distinguish live and dead cell populations.

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Lab products found in correlation

Human fc block, by BD (3 mentions) Pd l1 bv421, by BD (3 mentions) Cytofix, by BD (3 mentions) Facscanto, by BD (3 mentions) Cd133 apc, by BD (2 mentions) Egfr pe, by BD (2 mentions) Cd24 bv711 ml5, by BD (2 mentions) Cd15 pe 6d4, by BD (2 mentions) Pd l1 bv605, by BioLegend (2 mentions) B7 h6 alexafluor700, by R&D Systems (2 mentions) Lsrfortessa, by BD (2 mentions) Hla abc apc, by BioLegend (1 mentions) Mica b pe cy7 6d4, by BioLegend (1 mentions)

Close spelling variants of this product

LIVE/DEAD stain (166 citations) Fixable live/dead stain (61 citations) Live/Dead Fixable Green (11 citations) LIVE/DEAD Green Dead Cell Stain Kit (2 citations) Fixable live/dead (2 citations)

3 protocols using live dead 488 fixable green dead cell stain kit

Comprehensive Cell Marker Analysis in Chordoma

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For general cell surface marker analysis, chordoma cells were labeled with LIVE/DEAD 488 Fixable Green Dead Cell Stain Kit (Life Technologies #L34970) according to the manufacturer’s protocol. Cells were then washed and resuspended in FACS buffer (PBS+1% BSA). Human Fc block (BD #564219) was added to 1×10⁶ cells and incubated for 10 minutes on ice. Cells were stained with HLA-A,B,C-APC (BioLegend #311410), PD-L1-BV421 (BD #563738), or EGFR-PE (BD #555997) and fixed with Cytofix (BD #554655). Marker expression was quantified by percent positive cells and mean fluorescence intensity (MFI). Flow cytometry was performed on BD FACSCanto (BD Biosciences) and analyzed using FlowJo v10.7.1 (TreeStar).
The CSC population in the UM-Chor1 cell line was identified as CD24⁺CD15⁺CD133⁺ cells using the following antibodies from BD Biosciences: CD24-BV711 (ML5), CD15-PE (6D4), and CD133-APC (W6D3). To stain for surface markers, PD-L1-BV605 (MPC11, BioLegend), MICA/B-PECy7 (6D4, BioLegend), HLA-A,B,C-BV605 (W6/32, BioLegend) and B7-H6-AlexaFluor700 (875001, R&D) antibodies and their appropriate isotype controls were used. Flow cytometry was performed on BD LSRFortessa (BD Biosciences) and analyzed using FlowJo V.10.7.1 (TreeStar).

Hoke A.T., Padget M.R., Fabian K.P., Nandal A., Gallia G.L., Bilusic M., Soon-Shiong P., Hodge J.W, & London NR J.r. (2021). Combinatorial Natural Killer Cell–based Immunotherapy Approaches Selectively Target Chordoma Cancer Stem Cells. Cancer Research Communications, 1(3), 127-139.

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Chordoma Cell Surface Marker Analysis

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For general cell surface marker analysis, chordoma cells were labeled with LIVE/DEAD 488 Fixable Green Dead Cell Stain Kit (Life Technologies, #L34970) according to the manufacturer's protocol. Cells were then washed and resuspended in FACS buffer (PBS + 1% BSA). Human Fc block (BD #564219) was added to 1 × 10⁶ cells and incubated for 10 minutes on ice. Cells were stained with HLA-A, B, C-APC (BioLegend #311410), PD-L1-BV421 (BD #563738), or EGFR-PE (BD #555997) and fixed with Cytofix (BD #554655). Marker expression was quantified by percent positive cells and mean fluorescence intensity (MFI). Flow cytometry was performed on BD FACSCanto (BD Biosciences) and analyzed using FlowJo v10.7.1 (TreeStar).
The CSC population in the UM-Chor1 cell line was identified as CD24⁺CD15⁺CD133⁺ cells using the following antibodies from BD Biosciences: CD24-BV711 (ML5), CD15-PE (6D4), and CD133-APC (W6D3). To stain for surface markers, PD-L1-BV605 (MPC11, BioLegend), MICA/B-PECy7 (6D4, BioLegend), HLA-A, B, C-BV605 (W6/32, BioLegend) and B7-H6-AlexaFluor700 (875001, R&D Systems) antibodies and their appropriate isotype controls were used. Flow cytometry was performed on BD LSRFortessa (BD Biosciences) and analyzed using FlowJo V.10.7.1 (TreeStar).

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PD-L1 Expression Profiling of SNUC Cells

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For assessment of survival, SNUC cells were labeled with LIVE/DEAD 488 Fixable Green Dead Cell Stain Kit (Life Technologies #L34970) according to the manufacturer's protocol. Human Fc block (BD #564,219) was added to 1 × 10⁶ cells and incubated for 10 min on ice. To characterize PD-L1 expression in the SNUC cell lines, cells were stained with PD-L1-BV421 (BD #563,738) and fixed with Cytofix (BD #554,655). Marker expression was quantified by percent positive cells and mean fluorescence intensity (MFI). Flow cytometry was performed on BD FACSCanto (BD Biosciences) and analyzed using FlowJo v10.7.1 (TreeStar).

Hoke A.T., Takahashi Y., Padget M.R., Gomez J., Amit M., Burks J., Bell D., Xie T., Soon-Shiong P., Hodge J.W., Hanna E.Y, & London NR J.r. (2024). Targeting sinonasal undifferentiated carcinoma with a combinatory immunotherapy approach. Translational Oncology, 44, 101943.

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