Chk2 inhibitor 2 hydrate

Manufactured by Merck Group

CHK2 inhibitor II hydrate is a chemical compound that serves as an inhibitor of the CHK2 protein, which is involved in the cellular response to DNA damage. This product is commonly used in research applications to study the role of CHK2 in various biological processes.

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Lab products found in correlation

Ucn 01, by Merck Group (1 mentions) Sb218078, by Merck Group (1 mentions) Sc 15408, by Santa Cruz Biotechnology (1 mentions) Ve 821, by Merck Group (1 mentions) Aphidicolin, by Merck Group (1 mentions) Reversine, by Cayman Chemical (1 mentions) Nocodazole, by Merck Group (1 mentions) Ro 3306, by Merck Group (1 mentions) Nms p715, by Merck Group (1 mentions) Monastrol, by Bio-Techne (1 mentions) Sb203580, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Chk2 inhibitor II (14 citations) Chk2 inhibitor (7 citations) Inhibitor II (2 citations)

2 protocols using chk2 inhibitor 2 hydrate

Antibody-based Detection of DNA Damage Markers

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Primary antibodies used were rabbit antibodies against ATRX (sc-15408, Santa Cruz), H3.3 (09–838, Millipore Merck), phosphorylated H3.3S31 (ab2889, Abcam; Active Motif, 39637; H3.3S31ph) (Abcam; Active Motif), CHK1 Ser317 (12302, Cell Signaling Technology), CHK2 Thr68 (abe343, Cell Signaling Technology), CHK1 (NBP1–19210, Novus Biologicals) and CHK2 (05–649, Millipore Merck); mouse monoclonal antibodies against γH2AX Ser139 (05–636, Millipore Merck), and myc tag (05–724, Millipore Merck), tubulin (Roche), and human anti centromere CREST serum (34 (link)). CHK1 inhibitor UCN-01 (Millipore Merck) and SB218078 (Millipore Merck) were used at a concentration of 1 μM and 5 μM, respectively (36 (link)). CHK2 inhibitor II hydrate (Sigma Aldrich) was used at a final concentration of 250 μM.

Chang F.T., Chan F.L., R. McGhie J.D., Udugama M., Mayne L., Collas P., Mann J.R, & Wong L.H. (2015). CHK1-driven histone H3.3 serine 31 phosphorylation is important for chromatin maintenance and cell survival in human ALT cancer cells. Nucleic Acids Research, 43(5), 2603-2614.

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Pharmacological Inhibitors in Cell Research

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Reversine was obtained from Cayman Chemical or Sigma-Aldrich and used at a working concentration of 0.5 μM or 2μM; Monastrol (working concentration 100 μM) from Tocris; SB203580 (working concentration 10 μM) from CellSignalingTechnology; Thymidine (working concentration 5 mM), Aphidicolin (working concentration 400 nM), RO-3306 (working concentration 7.5 μM), Chk2 inhibitor II hydrate (working concentration 10 μM), VE821 (working concentration 1 μM) and Nocodazole (working concentration 330 nM) were obtained from Sigma-Aldrich. NMS-P715 (working concentration 1 μM) was obtained from EMD/Millipore.

Santaguida S., Richardson A., Iyer D.R., M’Saad O., Zasadil L., Knouse K.A., Wong Y.L., Rhind N., Desai A, & Amon A. (2017). Chromosome mis-segregation generates cell cycle-arrested cells with complex karyotypes that are eliminated by the immune system. Developmental cell, 41(6), 638-651.e5.

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