Isolated BMDMCs were characterized by flow-cytometry analysis of specific surface antigens. Cells were incubated for 20 min at room temperature with primary antibodies conjugated with fluorescein isothiocyanate (FITC), phycoerythrin (PE), allophycocyanin (APC), peridinin-chlorophyll-protein (PercP), and phycoerythrin/cyanine 7 (PE/Cy7). The markers tested included: pan-leukocyte, CD45 (Immunostep); pan T cell, CD3 (BD Biosciences Pharmingen); cytotoxic T cell, CD8 (BD Biosciences Pharmingen); helper T cell, CD4 (BD Biosciences Pharmingen); pan B cell, CD19 (BD Biosciences Pharmingen); NK cell, CD56 (BD Biosciences Pharmingen); promonocyte, CD64 (Immunotech); monocyte, CD14 (IQP); hematopoietic progenitor cells, CD117 (BD Biosciences Pharmingen) and CD34 (BD Biosciences Pharmingen); mesenchymal cells, CD105 (Immunostep), CD73 (BD Biosciences Pharmingen), and CD90 (BD Biosciences Pharmingen); neutrophils, CD31 (BD Biosciences Pharmingen) and CD33 (BD Biosciences Pharmingen); and anti-HLA-DR (MHC-II, BD Biosciences Pharmingen). After staining, erythrocytes were lysed with B&D Lysis Buffer Solution. Data were acquired on a BD FACS CANTO cytometer (BD Biosciences) and analyzed with BD Paint-a-Gate software.
Cd105
Manufactured by Immunostep
Sourced in United States
CD105, also known as endoglin, is a cell surface glycoprotein that is primarily expressed on endothelial cells. It functions as a co-receptor for the transforming growth factor beta (TGF-β) signaling pathway, playing a role in angiogenesis and vascular development.
Automatically generated - may contain errors
4 protocols using cd105
1
Characterization of Isolated Bone Marrow Derived Mononuclear Cells
2
Multimarker Immunophenotyping of Cell Lines
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OS cell lines or CAR T cells were stained with fluorochrome-labeled antibodies against human B7-H3 (EPNCIR122 from Abcam; clone 8H9 from hybridoma cell line), EGFR (R&D Systems), CD45 (Biolegend), CD29 (eBioscience), CD166 (AbD Serotec) and CD105 (Immunostep). Data were analyzed by Quant Analyzer 10 (Miltenyi Biotech) and FlowJo software.
3
Mesenchymal Stem Cell Immunophenotyping
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Isolated stromal cells were trypsinized and centrifuged.
The cell pellet was resuspended in PBS supplemented with
5% FBS and incubated with monoclonal antibodies for 30
minutes at 4°C in the dark. Human CD45 (BD Bioscience,
USA) and CD34 (IMMUNOSTEP, Spain) antibodies
were served as negative controls, while anti-human
CD90 (BD Bioscience, USA), CD105 (IMMUNOSTEP,
Spain), CD73 (BD Bioscience, USA) and CD146 (BD
Bioscience, USA) were used as specific antibodies. Cells
were evaluated with a FACS Calibur apparatus (Becton
Dickinson, USA). Finally, the analysis was done using
FlowJo 7.6 software.
The cell pellet was resuspended in PBS supplemented with
5% FBS and incubated with monoclonal antibodies for 30
minutes at 4°C in the dark. Human CD45 (BD Bioscience,
USA) and CD34 (IMMUNOSTEP, Spain) antibodies
were served as negative controls, while anti-human
CD90 (BD Bioscience, USA), CD105 (IMMUNOSTEP,
Spain), CD73 (BD Bioscience, USA) and CD146 (BD
Bioscience, USA) were used as specific antibodies. Cells
were evaluated with a FACS Calibur apparatus (Becton
Dickinson, USA). Finally, the analysis was done using
FlowJo 7.6 software.
4
Mesenchymal Cell Characterization by Flow Cytometry
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Mesenchymal cells were digested enzymatically by trypsin and centrifuged to obtain the cell pellet, which was resuspended again in 5% FBS and incubated in a dark place for 30 minutes at 4°C in monoclonal antibodies. Negative control antibodies (human CD45, BD Biosciences, and CD34, IMMUNOSTEP, Spain) and specific antibodies (anti-human CD90 (BD Biosciences, USA), CD105 (IMMUNOSTEP, Spain), CD73 (BD Biosciences, USA), and CD146 (BD Biosciences, USA)) were used to cells' assessment by a FACS Calibur apparatus (Becton Dickinson, USA).
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