Hippocampus preparation and Western blot were performed as described previously [13 (link), 20 (link)]. Briefly, each hippocampus was dissected immediately after sacrificing the animal and was stored at − 80 °C until use. For immunoblot analysis, the frozen hippocampi were homogenized in ice-cold 50 mM Tris-HCl buffer (pH 7.4) containing 1% Triton X-100, 0.2 mM PMSF, and 1 mM EDTA. Homogenates were centrifuged at 12,000×g for 10 min at 4 °C. The supernatants obtained were immediately placed in boiling water for 10 min. Samples (10 μg protein/lane) were separated on 10% SDS-PAGE gels and electrophoretically transferred to nitrocellulose membranes. The membranes were probed with rabbit antibodies against the following proteins: Tau (C-17) (polyclonal, 1:1000, Bioworld Technology, China), PS202 (polyclonal, 1:1000, Bioworld Technology, China), PS404 (polyclonal, 1:1000, Bioworld Technology, China), Egr1 (polyclonal, 1:1000, Bioworld Technology, China), and c-Fos (polyclonal, 1:1000, Bioworld Technology, China). Anti-GAPDH (1:10,000; Bioworld Technology) was used as the internal reference standard.
C fos
Manufactured by Bioworld Technology
Sourced in China
C-Fos is a protein that serves as an indicator of neuronal activation. It is commonly used in laboratory research to identify and map patterns of neuronal activity in the brain and other tissues.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh, by Bioworld Technology (1 mentions)
C jun, by Abcam (1 mentions)
P c jun, by Abcam (1 mentions)
Nur77, by Abcam (1 mentions)
3β hsd, by Santa Cruz Biotechnology (1 mentions)
P450scc, by Cell Signaling Technology (1 mentions)
Sr b1, by Abcam (1 mentions)
17β hsd, by Santa Cruz Biotechnology (1 mentions)
β tubulin, by Cell Signaling Technology (1 mentions)
P jnk, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
β actin, by Abcam (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
2 protocols using c fos
1
Hippocampus Protein Analysis by Western Blot
2
Protein Extraction and Western Blot Analysis
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Proteins were extracted from rat testes and TM3 cells using Cell Lysis Buffer for Western blot and IP. Concentrations of protein were determined using BCA protein assay kits (Beyotime Biotech Inc., China). Twenty μg of protein were separated and electrophoresed by SDS-PAGE. Nitrocellulose membranes were blocked by TBST containing 5% non-fat milk for 1 h at 37 °C, followed by incubations with specific primary antibodies overnight at 4 °C and HRP-conjugated secondary antibodies for 1 h at 37 °C. Target proteins were visualized and analyzed using the ECL system. ERK, p-ERK, JNK, p-JNK, p38, p-p38, P450scc, GAPDH, and β-Tubulin antibodies were bought from the Cell Signaling Technology Inc. (USA). Map3k1, SRD5A2, Nur77, c-Jun, p-c-Jun, SRB1, StAR, P450c17, and β-Actin antibodies were gotten from the Abcam Inc. (USA). AR, ERα, SF-1, 3β-HSD, and 17β-HSD antibodies were purchased from the Santa Cruz Biotechnology Inc. (USA). SRD5A1, c-Fos, and p-c-Fos antibodies were obtained from the Bioworld Technology Inc. (China).
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