End repair kit

Manufactured by Illumina

The End-repair kit is a laboratory reagent used in DNA sample preparation workflows. It is designed to repair the ends of DNA fragments, preparing them for subsequent processing steps such as adapter ligation or library construction. The kit contains the necessary enzymes and buffers to convert DNA ends into a format suitable for downstream applications.

Automatically generated - may contain errors

Lab products found in correlation

Hiseq 2000, by Illumina (2 mentions) Ampure xp bead, by Beckman Coulter (2 mentions) Iproof, by Bio-Rad (2 mentions) Paired end adapters, by Qiagen (2 mentions) Pe 102 1003, by Qiagen (2 mentions) Ultrapure ligase l603 hc l, by Qiagen (2 mentions) Dneasy blood and tissue kit, by Covaris (2 mentions)

Spelling variants of this product

End-It DNA End-Repair Kit (40 citations) End-It DNA-Repair kit (13 citations) End-It Repair Kit (10 citations)

2 protocols using end repair kit

Illumina Sequencing Library Preparation

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DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit and sheared using a Covaris E220 (Woburn, MA) (duty cycle 10, intensity 5, cycles/burst 200 and time 180 s) to ~400 base pair (bp). Sheared genomic DNA was gel purified and used as input for Illumina sequencing library prep, with end-repair using the NEBNext end-repair kit (E6050L), and A-tailing with Taq polymerase. Ligation to Illumina paired-end adapters (PE‐102-1003) was done using Ultrapure ligase (L603-HC-L) from Enzymatics, and amplification with iProof (Bio-Rad). Agencourt Ampure XP beads were used for clean-up and size selection at each step. The resulting DNA library was sequenced on a single lane of an Illumina HiSeq2000 at the Stanford Center for Genomics and Personalized Medicine.

Kelley J.L., Peyton J.T., Fiston-Lavier A.S., Teets N.M., Yee M.C., Johnston J.S., Bustamante C.D., Lee R.E, & Denlinger D.L. (2014). Compact genome of the Antarctic midge is likely an adaptation to an extreme environment. Nature Communications, 5, 4611.

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DNA Extraction, Shearing, and Library Prep

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DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit and sheared using a Covaris E220 (Woburn, MA) (duty cycle 10, intensity 5, cycles/burst 200, time 180 sec) to approximately 400 base pair (bp). Sheared genomic DNA was gel purified and used as input for Illumina sequencing library prep, with end-repair with NEBNext end-repair kit (E6050L), and A-tailing with Taq polymerase. Ligation to Illumina paired-end adapters (PE-102-1003) was done using Ultrapure ligase (L603-HC-L) from Enzymatics, and amplification with iProof (BioRad). Agencourt Ampure XP beads were used for clean-up and size selection at each step. The resulting DNA library was sequenced on a single lane of an Illumina HiSeq2000 at the Stanford Center for Genomics and Personalized Medicine.

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