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Gapdh antibody

Manufactured by Zhongshan Golden Bridge Biotechnology
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Sourced in Denmark, China, United States

The GAPDH antibody is a laboratory reagent used to detect the presence and quantify the levels of the GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) protein in biological samples. GAPDH is a widely expressed and highly conserved enzyme involved in the glycolytic pathway. The GAPDH antibody can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and ELISA, to analyze GAPDH expression in cell lines, tissues, or other biological samples.

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Lab products found in correlation

Digital imaging system, by Bio-Rad (1 mentions) Enhanced chemiluminescence, by Thermo Fisher Scientific (1 mentions) P iκbα, by Cell Signaling Technology (1 mentions) P ikkα β, by Abcam (1 mentions) Horseradish peroxidase labeled secondary antibody, by Zhongshan Golden Bridge Biotechnology (1 mentions) P nf κb p65 antibody, by Santa Cruz Biotechnology (1 mentions) Nf κb p65, by Cell Signaling Technology (1 mentions) P gsk 3β, by Abcam (1 mentions) P akt1, by Abcam (1 mentions) P aurka, by Cell Signaling Technology (1 mentions) Snail, by Abcam (1 mentions) Twist, by Abcam (1 mentions) Ube2c, by Abcam (1 mentions) Aurka, by Abcam (1 mentions) Pvdf membrane, by Merck Group (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) β catenin, by Abcam (1 mentions) Gsk 3β, by Abcam (1 mentions) Lenti nc, by Genechem (1 mentions)

3 protocols using gapdh antibody

1

Protein Expression Analysis in Murine Kidneys

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After the kidneys were collected from the mice, an equivalent of protein was resolved on dodecyl sulfate (SDS)-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes. The antibodies used are shown below: AGEs and RAGE antibodies were from Abcam, UK; p-IKKα/β, IKKβ, IKKα, p-IκBα, IκBα and NF-κB p65 antibodies were from Cell Signaling Technology, USA; p-NF-κB p65 antibody was from Santa Cruz Biotechnology, Glostrup, Denmark and GAPDH antibody was from Beijing Zhong Shan Golden Bridge Biotechnology Co., Ltd., China, with a dilution of 1:1000. Horseradish peroxidase-labeled secondary antibody was from Beijing Zhong Shan Golden Bridge Biotechnology Co., Ltd., with a dilution of 1:10,000. The membranes were developed with enhanced chemiluminescence (Thermo Scientific, USA) and visualized using a digital imaging system (BIO-RAD Laboratories, Inc., USA).
Hong J., Wang X., Zhang N., Fu H, & Li W. (2018). d-ribose induces nephropathy through RAGE-dependent NF-κB inflammation. Archives of Pharmacal Research, 41(8), 838-847.
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2

Western Blot Analysis of EMT Markers

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UBE2C, AURKA, β-catenin, AKT1, p-AKT1, GSK-3β, p-GSK-3β, Slug, Snail and Twist antibodies were purchased from Abcam (Cambridge, UK). E-cadherin, N-cadherin and p-AURKA antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The GAPDH antibody was purchased from Zhongshan Golden Bridge Biotechnology (Beijing, China). Western blot analysis reagents were purchased from Sigma, PVDF membranes were purchased from Millipore Corp. (Bedford, MA, USA) and RIPA lysis buffer was purchased from Beijing Taike Biotechnology. Lentiviruses containing an UBE2C and AURKA inhibitor sequences (Lenti-si-UBE2C and Lenti-si-AURKA) or negative control (Lenti-NC) were obtained from Shanghai GeneChem Co., Ltd. (Shanghai, China).
Wang R., Song Y., Liu X., Wang Q., Wang Y., Li L., Kang C, & Zhang Q. (2017). UBE2C induces EMT through Wnt/β-catenin and PI3K/Akt signaling pathways by regulating phosphorylation levels of Aurora-A. International Journal of Oncology, 50(4), 1116-1126.
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3

Immunoblotting of SOCS1, p-STAT1

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The procedure details were referred to our previous research [8] (link). SOCS1 antibody was obtained from Santa Cruz Biotechnology (USA). mCherry antibody was from Ythx Biotechnology (Beijing, China). Phosphorylated-STAT1 (p-STAT1) and STAT1 antibodies were purchased from Cell Signaling Technology (MA, USA) and GAPDH antibody was from ZhongShan Golden Bridge (Beijing, China).
He J., Ji Y., Li A., Zhang Q., Song W., Li Y., Huang H., Qian J., Zhai A., Yu X., Zhao J., Shang Q., Wei L, & Zhang F. (2014). MiR-122 Directly Inhibits Human Papillomavirus E6 Gene and Enhances Interferon Signaling through Blocking Suppressor of Cytokine Signaling 1 in SiHa Cells. PLoS ONE, 9(9), e108410.
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