Mouse il 2 elisa kit

The secretion of interleukin‐2 (IL‐2), interleukin‐10 (IL‐10), tumor necrosis factor‐α (TNF‐α), and interferon‐γ (IFN‐γ) were assessed by ELISA kits. The human IL‐2 ELISA Kit (Abcam, ab174444), human IL‐10 ELISA Kit (Abcam, ab46034), human TNF‐α ELISA Kit (Abcam, ab181421), and human IFN‐γ ELISA Kit (Abcam, ab46025) were used to detect the concentrations in the cell supernatant. The mouse IL‐2 ELISA Kit (Abcam, ab100716), mouse TNF‐α ELISA Kit (Abcam, ab100747), and mouse IFN‐γ ELISA Kit (Abcam, ab100689) were used to determine the concentrations in the mouse serum samples. The absorbance (OD) was measured at a wavelength of 450 nm.