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Anti twist1 2

Manufactured by GeneTex
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The Anti-Twist1/2 is a laboratory equipment used for the detection and quantification of the Twist1 and Twist2 proteins. It functions by utilizing antibodies specifically designed to bind to these proteins, allowing researchers to analyze their expression levels in various biological samples.

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Lab products found in correlation

Anti vimentin, by GeneTex (2 mentions) Anti zeb1, by GeneTex (2 mentions) Anti n cadherin, by GeneTex (1 mentions) Anti e cadherin, by GeneTex (1 mentions) Anti slug, by GeneTex (1 mentions) Anti γh2ax, by GeneTex (1 mentions) Anti gapdh, by Thermo Fisher Scientific (1 mentions) T per tissue protein extraction reagent, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Anti cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions) Anti n cadherin, by Abcam (1 mentions) Anti ki67, by Cell Signaling Technology (1 mentions) Anti cyclin d1, by Novus Biologicals (1 mentions) Anti gapdh mab374, by Merck Group (1 mentions) Anti foxm1, by GeneTex (1 mentions) Anti cd44, by GeneTex (1 mentions)

Close spelling variants of this product

TWIST1 (4 citations) Twist1/2 (3 citations)

3 protocols using anti twist1 2

1

Protein Expression Analysis of Tumor Tissues

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Tumors were harvested from the tumor-bearing mice after 4 weeks of treatment and then lysed in T-PER™ Tissue Protein Extraction Reagent (Thermo Fisher Scientific, Waltham, MA, USA) containing 1% protease inhibitor cocktail (Sigma-Aldrich Co., St. Louis, MO, USA). The procedures of Western blot analysis have been reported previously45 (link). The primary antibodies used in this study included anti-N-cadherin (GTX100443), anti-E-cadherin (GTX100443), anti-Twist1/2 (GTX127310), anti-ZEB-1 (GTX105278), anti-vimentin (GTX100619), anti-Slug (GTX128796), anti−γ-H2AX (GTX628789; GeneTex, Inc., Irvine, CA, USA), and anti-GAPDH (MA5-15738; Thermo Fisher Scientific, Waltham, MA, USA).
Chang C.Y., Chen C.C., Lin L.T., Chang C.H., Chen L.C., Wang H.E., Lee T.W, & Lee Y.J. (2018). PEGylated liposome-encapsulated rhenium-188 radiopharmaceutical inhibits proliferation and epithelial–mesenchymal transition of human head and neck cancer cells in vivo with repeated therapy. Cell Death Discovery, 4, 100.
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2

Histological Analysis of Tumor Samples

Cited 1 time
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Histological analysis of primary tumors was performed for a randomly selected subset of CTRL and miR-127PD tumors. Immunohistochemistry was performed as described (21 (link)). Anti-Ki-67 (1:500) (Cell Signaling Technologies, Cat# 9027), anti-cleaved Caspase 3 [Asp175] (1:500) (Cell Signaling Technologies, Cat# 9661), anti-human mitochondria [113-1] (1:1000) (Abcam, Cat# ab92824), anti-CyclinD1 (Novus Biologicals, Cat# NB100-79920) (1:100), anti-Vimentin (Gentex, Cat# GTX100619) (1:300), anti-Twist1/2 (Genetex, Cat# GTX127310) (1:200), and anti-N-Cadherin (Epitomics, Cat# 2447-1) (1:100), primary antibodies were used. An internal negative control (no primary antibody) was included with each trial. Images of 4 – 6 randomized fields of view per animal were captured then quantified using H DAB color deconvolution software in Image J Fiji (National Institutes of Health, RRID: SCR_002285). For analysis of tumor cell infiltration into lymph node tissue (n = 14), Image J software was used to quantify the total area of the lymph node compared to the total area comprised of tumor cells.
Umeh-Garcia M., Simion C., Ho P.Y., Batra N., Berg A.L., Carraway KL I.I.I., Yu A, & Sweeney C. (2019). A Novel Bioengineered MicroRNA-127 Prodrug Suppresses The Growth And Metastatic Potential Of Triple Negative Breast Cancer Cells. Cancer research, 80(3), 418-429.
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3

Western Blot Analysis of EMT Markers

Cited 1 time
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Western blot analysis was performed as described previously [52 (link),53 (link)]. The following antibodies were used: anti-FOXM1 (GTX100276), anti-BIRC5 (GTX100441), anti-AURKA (GTX13824), anti-CD44 (GTX102111), anti-TWIST1/2 (GTX127310), anti-ZEB1 (GTX105278), anti-CDH1 (GTX02618), anti-CK18 (GTX105624), anti-CK7 (GTX109723), and anti-VIMENTIN (GTX100619), which were obtained from GeneTex, Hsinchu City, Taiwan; anti-GAPDH (MAB374) and anti-SOX2 (AB5603), which were obtained from Merck Millipore (Burlington, MA, USA).
Wuputra K., Hsiao P.J., Chang W.T., Wu P.H., Chen L.A., Huang J.W., Su W.L., Yang Y.H., Wu D.C., Yokoyama K.K, & Kuo K.K. (2022). FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells. International Journal of Molecular Sciences, 23(14), 7782.
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