Trace dsq

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Trace DSQ is a gas chromatography–mass spectrometry (GC-MS) system designed for high-sensitivity and high-resolution analysis. It features a quadrupole mass analyzer for accurate quantitation and identification of compounds.

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Lab products found in correlation

78 protocols using trace dsq

Persistent Organic Pollutant Analysis

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PCBs, HCB and HCHs were analysed by gas chromatography with micro-electron capture detector (GC-μECD) (Agilent Technologies 6890 N). Endosulfans (α-and β-isomers and endosulfan sulfate) and PBDEs were determined by gas chromatography coupled to mass spectrometry in negative ion chemical ionization (GC-MS-NICI) and selective ion recording modes (Trace DSQ Instrument Thermo Electron). This technique was also used for structural confirmation of all OCs and for their quantification when the interferences in GC-μECD did not allow their determination. PAHs were analysed by gas chromatography coupled to mass spectrometry in electronic impact and selective ion recording modes (Trace DSQ Instrument Thermo Electron). Further details on instrumental analysis conditions and quantification of each pollutant family are described elsewhere (Arellano et al., 2014a (Arellano et al., , 2015 (link)(Arellano et al., , 2018)) .

Fernandez P., van Drooge B.L., Arellano L, & Grimalt J.O. (2021). Atmospheric deposition of semivolatile organic pollutants in European high mountains: Sources, settling and chemical degradation. The Science of the total environment, 784.

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Serum Metabolomic Profiling in Mice

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Blood were collected by cardiac puncture in Microvette^® tubes (Sarstedt, Marnay, France) from behaviorally validated adult mice. The tubes were centrifuged at 10,000 × g for 5 min, at room temperature, to extract the serum. Serum samples were then extracted and analyzed on gas chromatography tandem mass spectrometry (GC/MS), LC/MS, and LC/MS/MS platforms by Metabolon, Inc. (CA, USA), blinded to the experimental conditions. Protein fractions were removed by serial extractions with organic aqueous solvents, concentrated using a TurboVap system (Zymark) and vacuum dried. For LC/MS and LC/MS/MS, samples were reconstituted in acidic or basic LC-compatible solvents containing >11 injection standards and run on a Waters ACQUITY UPLC and Thermo-Finnigan LTQ mass spectrometer, with a linear ion-trap front-end and a Fourier transform ion cyclotron resonance mass spectrometer back-end. For GC/MS, samples were derivatized under dried nitrogen using bistrimethyl-silyl-trifluoroacetamide and analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. Chemical entities were identified by comparison to metabolomic library entries of purified standards. Following log transformation and imputation with minimum observed values for each compound, data were analyzed using two-way ANOVA with contrasts.

Chevalier G., Siopi E., Guenin-Macé L., Pascal M., Laval T., Rifflet A., Boneca I.G., Demangel C., Colsch B., Pruvost A., Chu-Van E., Messager A., Leulier F., Lepousez G., Eberl G, & Lledo P.M. (2020). Effect of gut microbiota on depressive-like behaviors in mice is mediated by the endocannabinoid system. Nature Communications, 11, 6363.

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GC-MS Protocol for Metabolite Derivatization

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The samples destined for GC/MS analysis were re-dried under vacuum desiccation for a minimum of 24 h prior to being derivatized under dried nitrogen using bistrimethyl-silyl-triflouroacetamide (BSTFA). The GC column was 5% phenyl and the temperature ramp was from 40° to 300°C in a 16-min period. Samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. The instrument was tuned and calibrated for mass resolution and mass accuracy on a daily basis. The information output from the raw data files was automatically extracted as discussed below.

Bais P., Beebe K., Morelli K.H., Currie M.E., Norberg S.N., Evsikov A.V., Miers K.E., Seburn K.L., Guergueltcheva V., Kremensky I., Jordanova A., Bult C.J, & Burgess R.W. (2016). Metabolite profile of a mouse model of Charcot–Marie–Tooth type 2D neuropathy: implications for disease mechanisms and interventions. Biology Open, 5(7), 908-920.

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Quantitative Analysis of Aromatic Hydrocarbon Degradation

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Toluene concentrations were determined
by headspace analyses with GC/MS (GC, Trace-DSQ; MS, Thermo Finnigan,
San Jose, CA) in selective ion monitoring mode with a fused-silica
capillary column DB-5 as previously described.^{9 (link)} Total concentrations of toluene in the cultures were calculated
from concentrations in the liquid phase and in the headspace using
the dimensionless air–water partitioning constant K_aw at 25 °C of 0.235.^{10 (link)} Adding naphthalene and 2-methylnaphthalene in 2,2,4,4,6,8,8-heptamethylnonane
as carrier phase prohibited a reliable direct quantification of the
substrate concentrations.^{8 (link)} Therefore, sulfate
consumption was monitored using the barium-gelatin method to indirectly
quantify naphthalene and 2-methylnaphthalene degradation.^{11 (link)} Iron(II) production was measured with the ferrozine
assay.^{12 (link)} Cell growth was monitored by microscopically
counting cell numbers.

Dong X., Jochmann M.A., Elsner M., Meyer A.H., Bäcker L.E., Rahmatullah M., Schunk D., Lens G, & Meckenstock R.U. (2017). Monitoring Microbial Mineralization Using Reverse Stable Isotope Labeling Analysis by Mid-Infrared Laser Spectroscopy. Environmental Science & Technology, 51(20), 11876-11883.

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GC/MS Derivatization and Analysis Protocol

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The samples destined for GC/MS analysis were redried under vacuum desiccation for a minimum of 24 h prior to being derivatized under dried nitrogen using bistrimethyl‐silyl‐trifluoroacetamide. The GC column was 5% phenyl, and the temperature ramp is from 40 to 300 °C in a 16‐min period. Samples were analysed on a Thermo‐Finnigan Trace DSQ fast‐scanning single‐quadrupole mass spectrometer using electron impact ionization. The instrument was tuned and calibrated for mass resolution and mass accuracy on a daily basis. The information output from the raw data files was automatically extracted as discussed below.

Hoang L.T., Domingo‐Sabugo C., Starren E.S., Willis‐Owen S.A., Morris‐Rosendahl D.J., Nicholson A.G., Cookson W.O, & Moffatt M.F. (2019). Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer. Molecular Oncology, 13(11), 2406-2421.

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GC-MS Protocol for Metabolite Derivatization

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The samples destined for GC/MS analysis were re-dried under vacuum desiccation for a minimum of 24 h prior to being derivatized under dried nitrogen using bistrimethyl-silyl-triflouroacetamide (BSTFA). The GC column was 5% phenyl and the temperature ramp was from 40 °C to 300 °C in a 16 min period. Samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. The instrument was tuned and calibrated for mass resolution and mass accuracy on a daily basis. The information output from the raw data files was automatically extracted as discussed below.

Stirling E.R., Cook K.L., Roberts D.D, & Soto-Pantoja D.R. (2019). Metabolomic Analysis Reveals Unique Biochemical Signatures Associated with Protection from Radiation Induced Lung Injury by Lack of cd47 Receptor Gene Expression. Metabolites, 9(10), 218.

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GC-MS Analysis of Derivatized Samples

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Samples were derivatized under nitrogen using bistrimethyl-silyltrifluoroacetamide and separated on a 5 % diphenyl/95 % dimethyl polysiloxane-fused silica column (20 m × 0.18 mm ID; 0.18-μm film thickness) with helium as carrier gas and a temperature ramp from 60 to 340 °C in a 17.5-min period. Internal standards amylbenzene, 1-phenylhexane, 1-phenyloctane, 1-phenyldecane, 1-phenyldodecane, hexadecylbenzene, octadecylbenzene, tetradecylbenzene, and 2,6-di-tert-butyl-4-methylphenol were added to each sample (250 ng of each standard per sample). Samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electro-impact ionization (EI) and operated at unit mass resolving power. The scan range was from 50–750 m/z.

Brown D.G., Rao S., Weir T.L., O’Malia J., Bazan M., Brown R.J, & Ryan E.P. (2016). Metabolomics and metabolic pathway networks from human colorectal cancers, adjacent mucosa, and stool. Cancer & Metabolism, 4, 11.

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GC-MS Analysis of Derivatized Samples

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The samples destined for GC/MS analysis were re-dried under vacuum desiccation for a minimum of 24 hours prior to being derivatized under dried nitrogen using bistrimethyl-silyl-triflouroacetamide (BSTFA). The GC column was 5% phenyl and the temperature ramp was from 40° to 300°C in a 16 min period. Samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. The instrument was tuned and calibrated for mass resolution and mass accuracy on a daily basis. The information output from the raw data files was automatically extracted as discussed below.

Theriot C.M., Koenigsknecht M.J., Carlson PE J.r., Hatton G.E., Nelson A.M., Li B., Huffnagle G.B., Li J, & Young V.B. (2014). Antibiotic-induced shifts in the mouse gut microbiome and metabolome increase susceptibility to Clostridium difficile infection. Nature communications, 5, 3114.

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Global Metabolomic Analysis of Mouse Liver

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The non-targeted global metabolomic analysis was carried out by Metabolon, Inc. (Durham, NC). Liver samples from mice that were fed the 13%-fat casein and the 13%-fat GMP diets were prepared using the automated MicroLab STAR system (Hamilton Company). Samples were either run on ultra-performance liquid chromatography (UPLC)-MS/MS or GC-MS. The LC/MS portion of the platform was based on a Waters ACQUITY UPLC and a Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer interfaced with a heated electrospray ionization source and Orbitrap mass analyzer operated at 35,000 mass resolution. Samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. Raw data was extracted, and compounds were identified by comparison to purified standards using Metabolon’s proprietary hardware and software.

Sawin E.A., Stroup B.M., Murali S.G., O’Neill L.M., Ntambi J.M, & Ney D.M. (2016). Differential Effects of Dietary Fat Content and Protein Source on Bone Phenotype and Fatty Acid Oxidation in Female C57Bl/6 Mice. PLoS ONE, 11(10), e0163234.

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GC-MS Sample Preparation and Analysis

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The samples destined for GC/MS analysis were re-dried under vacuum desiccation for a minimum of 24 hours prior to being derivatized under dried nitrogen using bistrimethyl-silyl-trifluoroacetamide (BSTFA). The GC column was 5% phenyl and the temperature ramp is from 40° to 300° C in a 16 minute period. Samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. The instrument was tuned and calibrated for mass resolution and mass accuracy on a daily basis. The information output from the raw data files was automatically extracted as discussed below.

Lucarelli G., Rutigliano M., Sallustio F., Ribatti D., Giglio A., Signorile M.L., Grossi V., Sanese P., Napoli A., Maiorano E., Bianchi C., Perego R.A., Ferro M., Ranieri E., Serino G., Bell L.N., Ditonno P., Simone C, & Battaglia M. (2018). Integrated multi-omics characterization reveals a distinctive metabolic signature and the role of NDUFA4L2 in promoting angiogenesis, chemoresistance, and mitochondrial dysfunction in clear cell renal cell carcinoma. Aging (Albany NY), 10(12), 3957-3985.

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