Bc03119a

Manufactured by Tissue Array

Sourced in United States

BC03119a is a laboratory equipment product. It is designed for conducting specific scientific procedures. The core function of this product is to assist in the research and analysis processes within a laboratory setting. However, a detailed description of its specific capabilities or intended use cannot be provided in an unbiased and factual manner without the risk of extrapolation.

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Lab products found in correlation

Il 6 antibody, by Abcam (1 mentions) Pgrn antibody, by Santa Cruz Biotechnology (1 mentions) Pv 6001, by ZSGB-BIO (1 mentions) Anti bmi1, by Cell Signaling Technology (1 mentions) Universal secondary antibody, by Roche (1 mentions) Dabmap, by Roche (1 mentions) Axiostar plus, by Zeiss (1 mentions)

4 protocols using bc03119a

Immunostaining of IL-6 and PGRN in HCC

Cited 1 time

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To detect the expression of IL-6 and PGRN in normal and HCC tissue, the human liver carcinoma and normal tissue microarrays (BC03119a, US Biomax, Inc., Rockville, MD) containing 95 HCC and 10 normal liver tissues were examined by immunohistochemical staining. This part of the study did not require approval from the Institutional Review Board as the tissue microarray was commercially sourced. Immunohistochemistry was performed as described59 (link) with IL-6 antibody (1:400, Abcam, Cambridge, UK) and PGRN antibody (1:50, Santa Cruz Biotechnology, Dallas, TX). Semi-quantitative analysis of the staining intensity score was as described22 (link).

Liu F., Zhang W., Yang F., Feng T., Zhou M., Yu Y., Yu X., Zhao W., Yi F., Tang W, & Lu Y. (2016). Interleukin-6-stimulated progranulin expression contributes to the malignancy of hepatocellular carcinoma cells by activating mTOR signaling. Scientific Reports, 6, 21260.

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Immunohistochemical Analysis of Liver Tissue

Cited 1 time

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Normal and cancerous human liver tissue microarrays (BC03119a) were obtained from U.S. Biomax (Rockville, MD, USA). AJAP1 and Ki-67 expression in tumor tissues was detected by immunohistochemistry as previously described.^{18 (link)}

Han J., Xie C., Pei T., Wang J., Lan Y., Huang K., Cui Y., Wang F., Zhang J., Pan S., Liang Y., Zhen T., Song R., Sun B., Li Y., Shi H., Yang G., Liu X., Zhu M., Wang Y., Li K., Liu Y., Meng F., Liao F., Meng X., Hong X, & Liu L. (2017). Deregulated AJAP1/β-catenin/ZEB1 signaling promotes hepatocellular carcinoma carcinogenesis and metastasis. Cell Death & Disease, 8(4), e2736-.

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Immunohistochemical Analysis of NS5ABP37 in Hepatocellular Carcinoma

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A tissue microarray containing specimens from 110 HCC cases of different stages and 10 normal pancreatic tissue samples was purchased from US Biomax (BC03119a, Rockville, USA). All study protocols for clinical specimen collection were approved by the Ethics Committee of Beijing Ditan Hospital (Beijing, China) for Clinical Research. Immunohistochemistry staining for NS5ABP37 detection was carried out with routine procedures using anti‐NS5ABP37 antibody (PAB20193, 1:20; Abnova, Colorado, USA) overnight at 4°C, and HRP‐conjugated anti‐rabbit antibody (PV‐6001; ZSGB‐BIO, Beijing, China) for 30 min. Slides were counterstained with hematoxylin and analyzed under a microscope (BX51; Olympus, Tokyo, Japan).

Feng S., Han M., Zhou L., Wang Q., Li Z., Li Y., Lu H., Liu T., Ma Y., Liu S, & Cheng J. (2017). NS5ABP37 inhibits liver cancer by impeding lipogenesis and cholesterogenesis. Cancer Science, 108(1), 12-22.

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Profiling BMI1 and CD45 in Liver Carcinoma

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A tissue microarray (TMA) of 110 liver carcinomas across different clinical stages and pathology grades, and 10 normal tissues (BC03119a), was obtained from US Biomax (Rockville, MD). Pathological diagnosis and detailed HCC patient’s specifications are included in Supplementary Table 2. The TMA slide was deparaffinized and antigen retrieval was performed using CC1 (Cell Conditioning Solution, Ventana Medical Systems, Tucson, AZ, Cat# 950–124). The primary antibodies anti-BMI1 (Cell Signaling, Danvers, MA, #6964) and anti-CD45 (Leica, Buffalo Grove, IL, Cat# PA0042) were applied in 1:50 dilution for 1 h at 37 °C, followed by universal secondary antibody (Ventana Medical Systems, #760–4205) staining and the chromogenic detection kit DABMap (Ventana Medical Systems). The slide was counterstained with hematoxylin, sections were observed through a Zeiss Axiostar plus (Zeiss, Jena, Germany) and images were taken using capture DC software or scanned at the Center for Biomedical Imaging and Informatics at Rutgers Cancer Institute of New Jersey (RCINJ). An automated computer-based scoring of the TMA was done.

Bartucci M., Hussein M.S., Huselid E., Flaherty K., Patrizii M., Laddha S.V., Kui C., Bigos R.A., Gilleran J.A., El Ansary M.M., Awad M.A., Kimball S.D., Augeri D.J, & Sabaawy H.E. (2017). Synthesis and Characterization of Novel BMI1 Inhibitors Targeting Cellular Self-Renewal in Hepatocellular Carcinoma. Targeted oncology, 12(4), 449-462.

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