To detect SARS-CoV-2 virus particles, either nasopharyngeal swabs (Copan Liquid Amies eSwabs) or pharyngeal lavage specimens were analyzed by RT–PCR. Specimens were subjected to cellular lysis and RNA extraction on a MagNA Pure 24 System (Roche) or QiaSymphony (Qiagen). Real-time RT–PCR was conducted using LightCycler Multiplex RNA Virus Master Mix on a Lightcycler 480 RT system (both Roche) or a ViiA7 system (Applied Biosystems). For SARS-CoV-2 analysis, the Sarbecovirus specific LightMix Modular SARS-CoV (COVID-19) E gene assay was used (TIB Molbiol). EAV control (TIB Molbiol) was used as extraction and internal PCR control. All (RT)-PCR reactions were performed according to manufacturer's protocol.
Lightcycler 480 rt system
Manufactured by Roche
Sourced in Germany
The Lightcycler 480 RT system is a real-time PCR instrument designed for quantitative analysis of nucleic acid samples. It provides precise and sensitive detection of target sequences. The system is capable of performing real-time PCR, melting curve analysis, and high-resolution melting analysis.
Automatically generated - may contain errors
Lab products found in correlation
Viia 7 system, by Thermo Fisher Scientific (2 mentions)
Qiasymphony, by Qiagen (2 mentions)
Magna pure 24 system, by Roche (2 mentions)
Liquid amies eswabs, by Copan (2 mentions)
Eav control, by TIB Molbiol (2 mentions)
Lightcycler multiplex rna virus master mix, by Roche (2 mentions)
Biofire filmarray system, by bioMérieux (1 mentions)
2 protocols using lightcycler 480 rt system
1
SARS-CoV-2 Detection by RT-PCR
2
SARS-CoV-2 Detection by RT-PCR
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To detect SARS-CoV-2 virus particles, either nasopharyngeal swabs (Copan Liquid Amies eSwabs, Brescia, Italy) or pharyngeal lavage specimens were analyzed by RT-PCR. Specimens were subjected to cellular lysis and RNA extraction on a MagNA Pure 24 System (Roche, Mannheim, Germany) or QiaSymphony (Qiagen, Hilden, Germany). Real-time RT-PCR was conducted using LightCycler Multiplex RNA Virus Master Mix on a Lightcycler 480 RT system (both Roche, Mannheim, Germany) or a ViiA7 system (Applied Biosystems, Foster City, USA).
For SARS-CoV-2 analysis, the Sarbecovirus specific LightMix Modular SARS-CoV (COVID-19) E gene assay was used (TIB Molbiol, Berlin, Germany). EAV control (TIB Molbiol, Berlin, Germany) was used as extraction and internal PCR control. Samples from control patients with acute respiratory disease were additionally analyzed molecularly on the Biofire Filmarray system (bioMérieux, Marcy-l’Étoile, France) with the respiratory panel or the pneumonia plus panel. All (RT)-PCR reactions were performed according to manufacturer’s protocol.
For SARS-CoV-2 analysis, the Sarbecovirus specific LightMix Modular SARS-CoV (COVID-19) E gene assay was used (TIB Molbiol, Berlin, Germany). EAV control (TIB Molbiol, Berlin, Germany) was used as extraction and internal PCR control. Samples from control patients with acute respiratory disease were additionally analyzed molecularly on the Biofire Filmarray system (bioMérieux, Marcy-l’Étoile, France) with the respiratory panel or the pneumonia plus panel. All (RT)-PCR reactions were performed according to manufacturer’s protocol.
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