Cell viability and cell count were analyzed 48 h after transfection using the Via1‐Cassette on the NucleoView NC‐200 (Chemometec, Allerod, Denmark). Moreover, proliferation of transfected H838 cells was monitored for 48 h by the live‐cell imaging system IncuCyte ZOOM (Essen BioScience, Ann Arbor, MI).
Nucleoview nc 200
Manufactured by ChemoMetec
Sourced in Denmark
The NucleoView NC-200 is a compact and automated cell counter that utilizes advanced image analysis technology to provide accurate cell counting and viability analysis. The instrument is designed for reliable and efficient cell enumeration across a wide range of cell types and samples.
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3 protocols using nucleoview nc 200
1
Cell Viability and Proliferation Assay
2
Cell Growth and Antibody Titer Monitoring
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During continued cultivation aliquots were withdrawn at different time points for cell counting and antibody titer determination. Determination of cell number, cell diameter and percentage of cell aggregation were done using an automated cell counter (Nucleocounter NC‐200, Chemometec, Allerod, Denmark) in the Via1‐Cassette (Chemometec). Data were analysed with the NucleoView NC‐200 software (Chemometec). For antibody titer determination an aliquot of the cell culture was centrifuged (13.000 rpm, 5 min) and the supernatant was analysed using a BLItz device (Fortebio) equipped with Protein A biosensors (Fortebio) for 60 s and 2200 rpm shaker speed. Antibody concentrations were determined using a corresponding calibration curve as described in the manufacturer´s manual.
3
Cell Viability and Proliferation Assay
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Cell viability (%) and proliferation were assessed after 24 hr of exposure. Immediately after harvesting, the total number of cells was counted by Nucleocounter NC‐200® (Chemometec A/S, Allerod Denmark) using Via1‐Cassettes (Cat No: 941‐0012, Chemometec A/S, Allerod Denmark). Data were analyzed with the enclosed computer software Chemometec Nucleoview NC‐200. For statistical analysis, the percentages of viable cells were transformed to arcsin‐values (n = 3). Cell proliferation was determined as the percentage change in the total number of cells. An effect on cell proliferation was only considered relevant if the effect was statistically significant and dose‐dependent across the dose range (0–200 µg/ml). To minimize variability across experiments, data for cell proliferation were normalized to the mean of the control level (0 µg/ml) for the respective experiment (n = 3).
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