Plasma levels of LXA4, 15-epi-LXA4, PGE2, and LTB4 were measured using the MyBioSource.com quantitative measurement kit and plasma levels of IL-1α, IL-1β, IFNγ, and TNFα were measured using Bio-Plex multiplex cytokine assay system (Bio-Rad, Hercules, CA). The lowest detection limits were as follows LXA4, 0.156 ng/mL; 15-epi-LXA4, 0.312 ng/mL; PGE2, 7.8 pg/mL; LTB4, 15.6 pg/mL; IL-1α, 5.23 pg/mL; IL-1β, 3.96 pg/mL; IFNγ, 4.39 pg/mL, and TNFα, 3.24 pg/mL.
Bioplex multiplex cytokine assay system
Manufactured by Bio-Rad
Sourced in Canada
The Bioplex multiplex cytokine assay system is a laboratory equipment designed to measure multiple cytokines and chemokines simultaneously in a single sample. It utilizes fluorescent-labeled beads coated with specific antibodies to capture and detect target analytes. The system enables efficient and accurate quantification of various immune-related proteins, providing researchers with a comprehensive analysis of complex biological samples.
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8 protocols using bioplex multiplex cytokine assay system
1
Quantification of Inflammatory Mediators
2
Multiplex Cytokine and Chemokine Profiling
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Circulating levels of cytokines and chemokines were measured using Bio-Plex multiplex assay system (Bio-Rad, Hercules, CA). Circulating levels of IFNγ, IL-2, TNFα, IL-17A, IL-22, IL-1β, IL-6, IL-12, GM-CSF, IL-4, IL-5, IL-13 and IL-10 were measured using the Bio-Plex multiplex cytokine assay system (Bio-Rad, Hercules, CA). TGFβ alone was measured using the R & D Systems Quantikine ELISA. The lowest detection limits were as follows: IFNγ, 4.39 pg/mL; IL-2, 29.63 pg/mL; TNFα, 3.24 pg/mL; IL-17A, 1.69 pg/mL; IL-22, 12.63 pg/mL; IL-1β, 3.96; IL-6, 7.7 pg/mL; IL-12, 4.66 pg/mL; GM-CSF, 12.22 pg/mL; IL-4, 14.61 pg/mL; IL-5, 6.63 pg/mL; IL-13, 216.2 pg/mL; IL-10, 4.65 pg/mL and TGFβ, 7.8 pg/mL. Luminex Human Chemokines Magnetic Assay kit (R & D systems) was used to measure the chemokine levels of CCL1, CCL2, CCL3, CCL4, CCL-11, CXCL1, CXCL2, CXCL9, CXCL10, and CXCL11. The lowest detection limits were as follows: CCL1, 1.57 pg/mL; CCL2, 31.8 pg/mL; CCL3, 90.9 pg/mL; CCL-4, 133.1 pg/mL, CCL11, 21.6 pg/mL; CXCL1, 49.2 pg/mL; CXCL2, 49.2 pg/mL; CXCL9, 600.6 pg/mL CXCL10, 2.88 pg/mL and CXCL11, 21.6 pg/mL.
3
Multiplex Cytokine Profiling in Plasma
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Circulating plasma cytokine levels were measured using a bio-plex multiplex cytokine assay system (Bio-Rad, Hercules, CA). The analytes measured were IFN-γ, TNF-α, IL-2, IL-4, IL-5, IL-13, IL-12, IL-17A, IL-22, IL-10, IL-1α, IL-1β, IL-6 and GM-CSF. The experiment was carried out according to the manufacturer’s instruction provided (R & D systems). The standard ranges of each cytokine were as follows: IFN-γ-49.18–11,950 pg/ml, TNF-α-8.89–2,160 pg/ml, IL-2-31.11–7,560 pg/ml, IL-4-15.72–3,820 pg/ml, IL-5-6.5–1,580 pg/ml, IL-13-367.78–89,370 pg/ml, IL-12-133.58–32,460 pg/ml, IL-17A-12.47–3,030 pg/ml, IL-22-14.28–3,470 pg/ml, IL-10-3.62–880 pg/ml, IL-1α-5.31–1,290 pg/ml, IL-1β-16.3–3,960 pg/ml, IL-6-4.53–1,100 pg/ml and GM-CSF-11.58–2,900 pg/ml.
4
Multiplex Cytokine Profiling in Plasma
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Plasma cytokines on all 50 individuals were measured using Bioplex multiplex cytokine assay system (Biorad). The cytokines analyzed were IL-2, IFN-γ, TNF-α, IL-10, IL-17A, IL-17F and IL-22. TGFβ levels were measured using a standard ELISA kit from R&D Systems.
5
Plasma Cytokine Profiling Assay
6
Multiplex Adipocytokine Profiling in Plasma
7
Multiplex Cytokine Profiling in Plasma
8
Plasma Cytokine and Chemokine Analysis
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Plasma cytokines and chemokines were measured using a Bioplex multiplex cytokine assay system (Bio-Rad, Hercules, CA). The parameters analyzed were IFNγ, TNFα, IL-2, IL-17A, IL-4, IL-5, IL-10, IL-6, IL-12 and GM-CSF. Plasma levels of TGFβ, IL-1α, IL-1β (all R& D Systems); IL-17F (Biolegend); IL-22 (eBioscience); Type 1 interferons (IFNs) - IFNα (multiple subtypes) and IFNβ (PBL Interferon Source) were measured by ELISA.
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