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Anti syk n 19

Manufactured by Santa Cruz Biotechnology
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Anti-Syk (N-19) is a primary antibody that recognizes the Syk (N-19) epitope. Syk is a protein tyrosine kinase involved in intracellular signal transduction pathways.

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Lab products found in correlation

Erk p44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) H3 anti histone h3 pan, by Merck Group (2 mentions) Psyk anti phospho zap 70 tyr319 syk tyr352, by Cell Signaling Technology (2 mentions) Perk erk1 2 ptpy185 187, by Thermo Fisher Scientific (2 mentions) Anti rac1, by Merck Group (1 mentions) Anti cd95 15a7, by Thermo Fisher Scientific (1 mentions) Anti cav 1 d46g3, by Cell Signaling Technology (1 mentions) Np bsa biotin, by Biosearch Technologies (1 mentions) Anti lyn, by Cell Signaling Technology (1 mentions) Anti cd19 6d5, by BioLegend (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti igm hrp, by Southern Biotech (1 mentions)

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Anti-Syk (12 citations)

3 protocols using anti syk n 19

1

Neutrophil Signaling Pathway Activation

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1×106 human peripheral neutrophils were plated in 12-well plates. Neutrophils were stimulated with C. albicans yeast or pre-formed hyphae. Add indicated times cells were lysed in SDS sample buffer and stored at −80°C. A standard immunoblotting protocol was carried out as described elsewhere. H3: anti-histone H3 (pan) (Millipore), Syk: anti-Syk (N-19) (Santa Cruz), pSyk: anti-phospho-Zap-70 (Tyr319)/Syk (Tyr352) (Cell Signaling), ERK: p44/42 MAPK (Erk1/2) (Cell Signaling), pERK: ERK1/2 (pTpY185/187) (Life Technologies).
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D, & Papayannopoulos V. (2014). Neutrophils sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nature immunology, 15(11), 1017-1025.
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2

Comprehensive B Cell Immunophenotyping

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The following antibodies were used for immunoblotting: anti-SYK (N-19; Santa Cruz), anti-IgM-HRP (Southern Biotech), anti-Cav1 (D46G3; Cell Signaling), anti-RAC1 (Millipore), anti-LYN (Cell signaling) and anti-Tubulin (Sigma).
The following antibodies were used for flow cytometry: anti-B220-PECy7 (RA3-6B2), anti-IgD (11-26), anti-IgM (polyclonal), anti-CD19 (1D3), anti-CD4 (GK1.5 and RM4-5), anti-CD8 (53-6.7), anti-CD93 (AA4.1), anti-CD69-PECy7 (H1.2F3), anti-CD86-PE (GL-1), anti-GL7, anti-CD95 (15A7) (all from eBioscience), anti-λLC λ1, λ2 & λ3 (R26-46), anti-κLC (187.1), anti-CD45.1 (A20), anti-IgD-FITC (11-26c.2a), anti-CD45.2 (105), Ki-67 (B56) (all from BD Pharmingen), anti-CD19 (6D5) (Biolegend). NP-BSA-biotin was from Biosearch Technologies. Anti-IgM-Fab’2-Alexa 649 was from Jackson ImmunoResearch (goat polyclonal). CSFE was from Sigma.
Minguet S., Kläsener K., Schaffer A.M., Fiala G.J., Osteso-Ibánez T., Raute K., Navarro-Lérida I., Hartl F.A., Seidl M., Reth M, & Del Pozo M.A. (2017). Caveolin-1-dependent BCR nanoscale organization regulates B cell tolerance. Nature immunology, 18(10), 1150-1159.
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3

Neutrophil Signaling Pathway Activation

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1×106 human peripheral neutrophils were plated in 12-well plates. Neutrophils were stimulated with C. albicans yeast or pre-formed hyphae. Add indicated times cells were lysed in SDS sample buffer and stored at −80°C. A standard immunoblotting protocol was carried out as described elsewhere. H3: anti-histone H3 (pan) (Millipore), Syk: anti-Syk (N-19) (Santa Cruz), pSyk: anti-phospho-Zap-70 (Tyr319)/Syk (Tyr352) (Cell Signaling), ERK: p44/42 MAPK (Erk1/2) (Cell Signaling), pERK: ERK1/2 (pTpY185/187) (Life Technologies).
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D, & Papayannopoulos V. (2014). Neutrophils sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nature immunology, 15(11), 1017-1025.
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