Sta liatest fm

The sodium citrate anticoagulant specimens from the first venous extraction of
patients (before thrombolytic or mechanical thrombectomy) upon admission to the
hospital were collected and centrifuged at 4,000 rpm for 10 min. Two to three
milliliters of plasma were transferred to EP tubes and stored at −80°C for
further testing.
Before detection, the plasma samples were placed in a water bath at 37°C for 15
min, and STA-R evolution automatic coagulation analyzer (Stago, France) was used
for FM and D-dimer detection. All operations were performed in accordance with
the manufacturer’s instructions of STA-Liatest FM (Stago, France) and D-dimer
Kit (Shanghai SUNBIO, China). The detection limit of FM is 5 µg/ml and the assay
working range is 5-150 µg/ml. The linearity range of D-dimer is 0.25-30 μg/ml
and the clinical reportable range is 0-30 μg/ml. For samples whose test results
were beyond the range reported by the instrument (FM > 150 μg/ml), the test
was repeated after manual dilution with normal saline.