The nanowire imaging was performed SEM in a FEI Inspect F50 microscope using the secondary electron mode. To proceed with compositional and microstructural experiments, nanowires were transferred from the Al2O3 template top surface to a carbon grid using a focused ion beam (FIB) Helios 660 manipulator. We used a JEM 2100 F TEM microscope to image the nanowires (bright field), map their composition through EDS (in six areas along the nanowire), and resolve their crystalline structure using SAED (taken in two zone axes in three different regions along the nanowire). On the other side, a FEI Quanta 650 FEG SEM allowed the TKD analysis (with 10 nm resolution), using a thick nanowire (d = 300 nm) to improve the electron inelastic scattering necessary to Kikuchi pattern formation. Subsequently, the pattern was indexed according to the crystalline phase obtained by the SAED analysis.
Inspect f50 microscope
Manufactured by Thermo Fisher Scientific
Sourced in Japan
The Thermo Fisher Scientific Inspect F50 is a scanning electron microscope designed for high-resolution imaging of samples. It provides detailed visualization of surface topography and microstructural features.
Automatically generated - may contain errors
Lab products found in correlation
Dsc 50 cell, by Shimadzu (2 mentions)
Quanta 650 feg sem, by Thermo Fisher Scientific (1 mentions)
Tecnai g2 spirit biotwin, by Thermo Fisher Scientific (1 mentions)
Cary 630, by Agilent Technologies (1 mentions)
Zetasizer nano s, by Malvern Panalytical (1 mentions)
Malvern zetasizer nano s, by Malvern Panalytical (1 mentions)
Cary 630 spectrophotometer, by Agilent Technologies (1 mentions)
Libra s22, by Harvard Bioscience (1 mentions)
Rotina 380 r benchtop, by Hettich (1 mentions)
6 protocols using inspect f50 microscope
1
Nanowire Characterization via Multitechnique Approach
2
Characterization of Porous PCL Scaffolds
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The pH of the degradation products was monitored by inserting a combination microelectrode (InLabMicro™, Toledo-Mettler) into the medium and following changes there with a digital pH meter (FiveEasy™, Toledo-Mettler). The form-stability of porous PCL external vascular scaffold was investigated by macroscopic observation and the microstructure change of which was observed by SEM with a FEI inspect F50 microscope (FEI, UAS). The mechanical properties of the PCL external vascular scaffolds were measured using an Instron 1121 universal testing machine (Instron, USA) with a crosshead speed of 50 mm/min. The tests were done on triplicate samples, and the results were presented as an average.
3
Nanoparticle Characterization by Electron Microscopy
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The morphological properties of designed nanoparticles were characterised by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). During TEM analysis, 10 µL of suspension of nanoparticles was placed on a carbon-coated grid for 1 min and dried with a filter paper before imaging. TEM images were obtained from the FEI Tecnai G2 Spirit BioTWIN with accelerating voltage at 80 kV. SEM samples were prepared by placing 2 mg of dried nanoparticles on SEM specimen stubs and coated with 10 nm Au. SEM images were obtained from FEI Inspect F50 Microscope operated at 10 kV.
4
Measuring TiO2 Film Thickness
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The thickness of TiO2 films (TiO2P and TiO2G) was determined by combining SEM imaging and SEM-EDAX (FEI Inspect F50 microscope) scans on cross-sections of the TiO2 samples. Cross-sectional images were recorded at a magnification of up to 100 k with 15 keV electron energy.
5
Physicochemical Characterization of Nanoparticles
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Dynamic Light Scattering (DLS) technique was used to obtain the mean size and polydispersity index (PDI) of the nanoparticles. Zeta potentials of the nanoparticles were measured by a Malvern Zetasizer Nano S. Scanning electron microscopy (SEM) images were taken using a Inspect F50 microscope (FEI Company) with field emission guns. Liquid samples were dropped on aluminum stubs covered with carbon tape, dried at 40 • C for 2 h, metalized with gold in the secondary electron mode, and then analyzed at 20 kV. Lyophilized nanoparticles and raw materials (HRP, IAA, CS and HA) were analyzed by Fourier-transformed infrared spectroscopy (FTIR) in spectrophotometer Cary 630 (Agilent Technologies), equipped with a zinc selenide crystal (ZnSe) and ATR (total attenuated reflection) device. Thermogravimetric analysis (TGA) was performed in SDT equipment model Q600 (TA Instruments): heating flow 10 • C/min (35 • C-600 • C) under nitrogen atmosphere (10 mL/ min). Differential scanning calorimetry (DSC) was performed in DSC-50 cell (Shimadzu): heating flow 10 • C/min (30 • C-500 • C) under nitrogen atmosphere (50 mL/min).
6
Nanoparticle Characterization Protocol
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For the preparation of nanoparticles: Magnetic stirrer (C-MAG HS 7, IKA -Staufen, Germany), pHmeter (D-22, Digimed -São Paulo, Brazil). Determination of Particle size, polydispersity index, and zeta potential: Malvern Zetasizer Nano S (Malvern Instruments, Worcestershire, UK). Enzymatic activity: UV/Vis Spectrophotometer (Libra S22, Biochrom -Cambridge, UK), centrifuge (Rotina 380 R Benchtop, Andreas Hettich GmbH & Co. KG -Tuttlingen, Germany). For Fourier transform infrared spectroscopy (FTIR), Thermogravimetric and Differential scanning calorimetry (DSC) analyzes were used: Cary 630 Spectrophotometer (Agilent Technologies -California, USA), SDT equipment model Q600 (TA Instruments -Delaware, USA), DSC-50 cell (Shimadzu -Kyoto, Japan), respectively. For Scanning electron microscopy (SEM) was used an Inspect F50 microscope (FEI Company -Tokyo, Japan).
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