After deparaffinization, rehydration, antigen retrieval, and blockade of endogenous peroxidase, tissues were incubated with anti-TUNEL (Roche), anti-cleaved Caspase 3 (Servicebio), anti-Ki67 (Servicebio), and anti-CD31 (Abways) primary antibodies at 4 °C overnight. Next, secondary antibodies were applied, and diaminobenzidine (DAB) (Dako) solution was used as a chromogen. Finally, the sections were counterstained with hematoxylin (Sigma-Aldrich) to identify nuclei.
Anti tunel
Manufactured by Roche
Anti-TUNEL is a laboratory equipment product designed for the detection and analysis of apoptosis, a form of programmed cell death. It provides a reliable method for the identification and quantification of cells undergoing apoptosis. The core function of Anti-TUNEL is to enable the visualization and assessment of DNA fragmentation, a hallmark of apoptotic cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti cleaved caspase 3, by Wuhan Servicebio Technology (2 mentions)
Diaminobenzidine dab, by Agilent Technologies (1 mentions)
Hematoxylin, by Merck Group (1 mentions)
Anti ki67, by Wuhan Servicebio Technology (1 mentions)
Dm4000b, by Leica (1 mentions)
Haematoxylin, by Merck Group (1 mentions)
Anti nsd2, by Abcam (1 mentions)
Ab15580, by Abcam (1 mentions)
3 protocols using anti tunel
1
Immunohistochemical Analysis of Apoptosis and Proliferation
2
Immunohistochemical Analysis of NSD2, TUNEL, and Caspase-3
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IHC was performed on 5-μm-thick formalin-fixed paraffin-embedded slices. After deparaffinization, rehydration, antigen retrieval and endogenous peroxidase inhibition, the samples were incubated with anti-NSD2 (Abcam), anti-TUNEL (Roche) and anti-cleaved caspase 3 (Servicebio) antibodies at 4 °C for 8–10 h. After the secondary antibody was applied, diaminobenzidine (DAKO) solution was used as a chromogen. Furthermore, haematoxylin (Sigma) staining was performed to identify nuclei. Images were acquired using a microscope (Leica DM 4000B).
3
Immunohistochemical Analysis of NSCLC Samples
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The tissue samples from NSCLC patients were embedded in liquid paraffin and cut into 4 μm sections. The primary antibodies were added at 4 °C overnight: anti-eIF5A2 (1:1000, ab227537, Abcam), anti-Ki67 (1:1000, ab15580, Abcam), anti-TUNEL (1:1000, Roche, Basel, Switzerland), anti-ATG3 (1:1000, ProteinTech). Next, after incubation with the corresponding secondary antibodies (1:2000, CST) at 37 °C for 30 min, the sections were stained with a 3, 3’-diaminobenzidine (DAB) plus kit based on the manufacturer’s protocol.
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