Standard solution (1.0 mg mL−1) of trenbolone (TREN) was obtained from Cerilliant (Round Rock, TX). Clenbuterol (CLEN), furosemide (FUR), hydrochlorothiazide (HCT), trichloro(3,3,3-trifluoropropyl)silane (TCTFPS), acetonitrile (99.9%, HPLC grade), methanol (99.9%, HPLC grade), ethyl acetate (99.8%, anhydrous), ethylene glycol, dimethyl sulfoxide, quinoline, and cyclohexanol were all purchased from Sigma-Aldrich (St. Louis, MO). Trichloromethylsilane (TCMS) and acetone were supplied by Fisher Scientific (Pittsburgh, PA, USA). Whatman filter paper grade 1 (24 cm) was purchased from Whatman (Little Chalfont, England).
Trichloro 3 3 3 trifluoropropyl silane
Manufactured by Merck Group
Sourced in United States, Macao
Trichloro(3,3,3-trifluoropropyl)silane is a chemical compound that functions as a silylating agent. It can be used in the synthesis of other chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Ethyl acetate, by Merck Group (2 mentions)
Clenbuterol, by Merck Group (1 mentions)
Quinoline, by Merck Group (1 mentions)
Acetone, by Thermo Fisher Scientific (1 mentions)
Hydrochlorothiazide, by Merck Group (1 mentions)
Furosemide, by Merck Group (1 mentions)
Whatman grade 1 filter paper, by Cytiva (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ethylene glycol, by Merck Group (1 mentions)
Cyclohexanol, by Merck Group (1 mentions)
Sylgard 184 silicone elastomer kit, by Dow (1 mentions)
Creatinine, by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Milli q advantage a10 water purification system, by Merck Group (1 mentions)
Reagent grade formic acid, by Merck Group (1 mentions)
Cocaine d3, by Cerilliant (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
4 protocols using trichloro 3 3 3 trifluoropropyl silane
1
Analytical method for SARM detection
2
Microfluidic Fluid Array Device Design
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The microwell
in the fluid array device was 100 μm in diameter and 150 μm
deep having 3000 arrays in a single device (3 × 3 cm). The spacing
between individual arrays on a device was redesigned from 4 ×
2 to 3 × 2 matrices based on the recovery efficiency of the glass
capillary extraction process in our previous work.22 (link) The SU-8 (Microchem 2075, Newton, MA, USA) master mold
was fabricated using standard photolithography processes. The processed
surface of a Si-wafer was silanized using trichloro(3,3,3-trifluoropropyl)
silane (Sigma-Aldrich, Korea) in a vacuum jar for 1 h. Polydimethylsiloxane
(PDMS, Sylgard 184 Silicone Elastomer Kit, Dow Corning, Midland, MI,
USA) was then cast, cured, and peeled off to prepare the fluid array
devices. To ensure that the environment was fully moisturized, the
demolded PDMS devices were dipped in distilled water for several hours,
resulting in a fully hydrated state (i.e., the highest solubility
of water into PDMS). This ensured that the aqueous fluid in the microwell
remained stable without apparent volume shrinkage over 24 h. During
long-term incubation (>12 h), the fluid array device was half-dipped
in oil and half-dipped in water to minimize array chamber drying.
in the fluid array device was 100 μm in diameter and 150 μm
deep having 3000 arrays in a single device (3 × 3 cm). The spacing
between individual arrays on a device was redesigned from 4 ×
2 to 3 × 2 matrices based on the recovery efficiency of the glass
capillary extraction process in our previous work.22 (link) The SU-8 (Microchem 2075, Newton, MA, USA) master mold
was fabricated using standard photolithography processes. The processed
surface of a Si-wafer was silanized using trichloro(3,3,3-trifluoropropyl)
silane (Sigma-Aldrich, Korea) in a vacuum jar for 1 h. Polydimethylsiloxane
(PDMS, Sylgard 184 Silicone Elastomer Kit, Dow Corning, Midland, MI,
USA) was then cast, cured, and peeled off to prepare the fluid array
devices. To ensure that the environment was fully moisturized, the
demolded PDMS devices were dipped in distilled water for several hours,
resulting in a fully hydrated state (i.e., the highest solubility
of water into PDMS). This ensured that the aqueous fluid in the microwell
remained stable without apparent volume shrinkage over 24 h. During
long-term incubation (>12 h), the fluid array device was half-dipped
in oil and half-dipped in water to minimize array chamber drying.
3
Creatinine Quantification Protocol
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Creatinine (≥ 98%), reagent-grade formic acid (≥ 95%) and trichloro(3,3,3-trifluoropropyl)silane (97%) were purchased from Sigma–Aldrich (St. Louis, Mo, USA). Creatinine-D3 (purity ≥ 95%) was obtained from Toronto Research Chemicals (Canada). Methanol (99.8%, HPLC grade) and defibrinated horse whole blood (Oxoid Deutschland) were purchased from Fisher Scientific (Loughborough, UK). Water was purified using a Milli-Q Advantage A10 water purification system (Millipore, MA, USA) before use in this study. Chromatography paper (25 mm, Grade 1) was purchased from Whatman (Maidstone, UK).
4
Cocaine Quantification in Biological Samples
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