Anti mettl14 antibody

Manufactured by Merck Group

Sourced in China, United States

The Anti-METTL14 antibody is a laboratory reagent used in research applications. It is designed to detect and bind to the METTL14 protein, which is involved in the methylation of RNA molecules. This antibody can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the METTL14 protein.

Automatically generated - may contain errors

Lab products found in correlation

Anti alkbh5 antibody, by Abcam (1 mentions) Anti fto antibody, by Abcam (1 mentions) Odyssey infrared scanner, by LI COR (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (1 mentions) β actin antibody, by Proteintech (1 mentions) Anti ythdf2 antibody, by Proteintech (1 mentions) Rabbit anti gfp antibody, by Merck Group (1 mentions) Rabbit anti phospho p38 antibody, by Cell Signaling Technology (1 mentions) Rabbit anti gapdh antibody, by Merck Group (1 mentions) Anti mettl3, by Merck Group (1 mentions) Anti p38 mapk, by Cell Signaling Technology (1 mentions) Anti irf3, by Cell Signaling Technology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti phospho irf3, by Cell Signaling Technology (1 mentions) Anti pten, by Cell Signaling Technology (1 mentions) Anti pres2, by Santa Cruz Biotechnology (1 mentions) Anti mettl3 antibody, by Proteintech (1 mentions) Anti ythdf3, by Santa Cruz Biotechnology (1 mentions) Anti flag, by Cell Signaling Technology (1 mentions)

3 protocols using anti mettl14 antibody

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein was extracted from the frozen clinical tissues, murine model tissues or cells with radioimmunoprecipitation assay (RIPA) Lysis Buffer and PMSF (Beyotime, Shanghai, China). The protein was electrophoresed on 7.5%-12% SDS–polyacrylamide gels according to the different proteins molecular weight and transferred to nitrocellulose membranes (Bio-Rad, Hercules, USA). The membranes were incubated with the anti-IL-17RA receptor antibody, anti-FTO antibody, anti-ALKBH5 antibody (Abcam, Cambridge, USA), anti-METTL3 antibody (Zen bio, China), anti-METTL14 antibody (Sigma-Aldrich, Germany), β-Actin antibody (Proteintech, USA) overnight at 4°C. And then the membranes were incubated with secondary antibody IRdye 680RD goat (polyclonal) anti-mouse IgG (H+L) and IRdye 800CW goat (polyclonal) anti-rabbit IgG (H+L) (Licor Biosciences, Nebraska, USA) for 2 hours at room temperature. The membranes were detected using an Odyssey infrared scanner (Licor Biosciences, Nebraska, USA). The antibodies are listed in Table S3.

Gan X., Dai Z., Ge C., Yin H., Wang Y., Tan J., Sun S., Zhou W., Yuan S, & Yang F. (2022). FTO promotes liver inflammation by suppressing m6A mRNA methylation of IL-17RA. Frontiers in Oncology, 12, 989353.

+ Open protocol

+ Expand

Western Blotting and Co-IP Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasmids are described in Supplementary Table S1. The following antibodies and reagents were used for western blotting assay, immunofluorescence, or Co-IP: rabbit anti-GAPDH antibody (Catalog#/Clone: SAB2701826/polyclonal), rabbit anti-GFP antibody (Catalog#/Clone: AB10145/polyclonal), anti-METTL14 antibody (Catalog#/Clone: SAB5700855/ polyclonal), anti-METTL3 (Catalog#/Clone: AV34590) were purchased from Sigma-Aldrich; anti-EsxB antibody (Catalog#/Clone: ab45074) and Anti-ESAT6 antibody (Catalog#/Clone: ab45073) were from Abcam; anti-CYBB-NOX2 antibody (Catalog#/Clone: NBP2-38642/polyclonal) was from Novusbio; rabbit anti-phospho-p38 antibody (Catalog#/Clone: 9215/3D7), anti-p38 MAPK (Catalog#/Clone: 8690/D13E1), were from Cell Signaling Technology, Danvers, MA; anti-YTHDF2 antibody (Catalog#/Clone: 24744-1-AP/polyclonal) was purchased from Proteintech; mouse monoclonal antibody to Mettl14 phosphorylated at Thr72 (p-T72-METTL14) was generated by immunization of rabbits, in collaboration with AbClonal Biotech.

Ma M., Duan Y., Peng C., Wu Y., Zhang X., Chang B., Wang F., Yang H., Zheng R., Cheng H., Cheng Y., He Y., Huang J., Lei J., Ma H., Li L., Wang J., Huang X., Tang F., Liu J., Li J., Ying R., Wang P., Sha W., Gao Y., Wang L, & Ge B. (2024). Mycobacterium tuberculosis inhibits METTL14-mediated m6A methylation of Nox2 mRNA and suppresses anti-TB immunity. Cell Discovery, 10, 36.

+ Open protocol

+ Expand

Reagents for HBV and m6A studies

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

HBV 1.3-mer was a kind gift from Dr. Wang-Shick Ryu (Yonsei University) and obtained from the Addgene (Cambridge, MA, USA). FLAG-YTHDF1, 2, and 3 plasmids were a kind gift from Dr. Stacy M. Horner (Duke University Medical Center) (15 (link)). Antibodies were obtained as follow: anti-HBc antibodies from Dr. Haitao Guo (University of Pittsburgh, PA, USA) (19 ), anti preS2, anti-YTHDF3, anti-GAPDH antibodies from Santa Cruz Biotechnology (Santa Cruz, CA, USA), anti-METTL3 antibody from Proteintech Group (Rosemont, IL, USA), anti-METTL14 antibody from Sigma-Aldrich, San Jose, CA, USA), anti-FTO and anti-YTHDF2 antibodies from Abcam (Cambridge, MA, USA), anti-FLAG, anti-PTEN, anti-IRF-3, and anti-phospho-IRF-3 antibodies from Cell Signaling Technology (Danvers, MA, USA).

Kim G.W., Imam H., Khan M., Mir S.A., Kim S.J., Yoon S.K., Hur W, & Siddiqui A. (2020). HBV-Induced Increased N6 Methyladenosine Modification of PTEN RNA Affects Innate Immunity and Contributes to HCC. Hepatology (Baltimore, Md.), 73(2), 533-547.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!