Hptlc plates silica gel 60 f 254

Manufactured by Merck Group

Sourced in Germany

HPTLC plates silica gel 60 F 254 are thin-layer chromatography plates coated with silica gel 60 and containing a fluorescent indicator F 254. The plates are designed for high-performance thin-layer chromatography (HPTLC) analysis.

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Lab products found in correlation

Acetylcholinesterase, by Merck Group (2 mentions) Butyrylcholinesterase bche, by Merck Group (2 mentions) Gallic acid, by Merck Group (2 mentions) Trolox, by Merck Group (2 mentions) Folin ciocalteu reagent, by Merck Group (2 mentions) Formic acid, by Merck Group (2 mentions) Acetonitrile, by Thermo Fisher Scientific (2 mentions) Fast blue b salt, by Merck Group (2 mentions) Modulyod freeze dryer, by Thermo Fisher Scientific (1 mentions) 100 μl syringe, by CAMAG (1 mentions) Automatic developing chamber adc 2, by CAMAG (1 mentions) Linomat 5, by CAMAG (1 mentions) Tlc visualizer, by CAMAG (1 mentions) Centrifuge 5804 r, by Eppendorf (1 mentions) Infinite m200, by Tecan (1 mentions) Multiwave go, by Anton Paar (1 mentions) 1 naphthyl acetate, by Merck Group (1 mentions) Optima lc ms grade methanol, by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Ethyl acetate, by Merck Group (1 mentions)

Spelling variants of this product

Silica gel 60 (932 citations) Silica gel 60 plates (129 citations) Silica gel plates (98 citations) HPTLC plates (53 citations) HPTLC silica gel 60 plates (29 citations) HPTLC Silica gel 60 (20 citations) Silica plates (15 citations) HPTLC silica gel plates (6 citations) Silica gel 60 F (5 citations) Silica Gel 60 HPTLC plates (4 citations)

7 protocols using hptlc plates silica gel 60 f 254

Quantitative Chemical Analysis Protocol

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Grinder (GT203840, Tefal, United Kingdom), Rotamixer (Hook and Tucker Instruments Ltd., United Kingdom), Grant XB22 ultrasonic bath (Grant Instruments, United Kingdom), Centrifugator (Centrifuge 5804 R, Eppendorf, Germany), electronic balance (Sartorius CP64, Sartorius AG, Germany), Freeze dryer (ModulyoD Freeze Dryer, Thermo Fisher Scientific, United Kingdom), NMR tube (VWR international Ltd., United States), Bruker Advance 500 MHz spectrometer (Bruker, Germany), HPTLC plates silica gel 60 F 254 (Merck, Germany), Linomat 5 (CAMAG, Switzerland) coupled with a 100 μL syringe (CAMAG, Switzerland) and compressed air with 60–90 psi., Automatic Developing Chamber ADC 2 (CAMAG, Switzerland), TLC Visualizer (CAMAG, Switzerland) Microwell plate Nunclon 96 well (Thermo Scientific Nunc, United Kingdom), GalaxyB CO₂ incubator (Scientific Laboratory Supplier Ltd., United Kingdom), water bath (LAUDA Aqualine AL 12, Germany), microscope (Olympus CK40 microscope, Japan), plate shaker (MS3 basic, IKA®, Germany) microplate reader (Infinite M200, Tecan, Switzerland), Multiwave Go (Anton Paar, Graz, Austria), ICP-OES SPECTROBLUE T1 (SPECTRO Analytical Instruments, Kleve, Germany).

Kum K.Y., Kirchhof R., Luick R, & Heinrich M. (2021). Danshen (Salvia miltiorrhiza) on the Global Market: What Are the Implications for Products’ Quality?. Frontiers in Pharmacology, 12, 621169.

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High-Performance Thin-Layer Chromatography

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The stationary phase used was Merck, HPTLC plates silica gel 60 F 254, with a plate format: 200.0 × 100.0 mm Application: Position Y: 8.0 mm, length: 4.0 mm, width: 0.0 mm Track: First position X: 12.0 mm, distance: 8.4 mm, Solvent front position: 85.0 mm.

Tessema F.B., Gonfa Y.H., Asfaw T.B., Tadesse M.G., Tadesse T.G., Bachheti A., Alshaharni M.O., Kumar P., Kumar V., Širić I., Abou Fayssal S., Chaubey K.K, & Bachheti R.K. (2023). Targeted HPTLC Profile, Quantification of Flavonoids and Phenolic Acids, and Antimicrobial Activity of Dodonaea angustifolia (L.f.) Leaves and Flowers. Molecules, 28(6), 2870.

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Antioxidant and Cholinesterase Inhibition Assays

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Fast Blue B salt, 2,2- diphenyl-1-picrylhydrazyl radical (DPPH, 97%), butyrylcholinesterase (BChE, from equine serum, ≥140 U/mg) and acetylcholinesterase (AChE, from Electrophorus electricus Linnæus, ≥245 U/mg), 1-naphthyl acetate, gallic acid, Trolox and solvents/reagents of analytical grade were purchased from Fluka Sigma-Aldrich, Schnelldorf, Germany. Bioluminescent marine Aliivibrio fischeri bacteria (NRRL-B11177, DSM no. 5171) were obtained from the German Collection of Microorganisms and Cell Cultures, Berlin, Germany. Ethanol, methanol and MS grade solvents (Optima LC-MS grade) methanol and acetonitrile were provided from Thermo Fisher Scientific, Schwerte, Germany. Bacillus subtilis spores (BGA, DSM 618 strain), formic acid (96%), hydrochloric acid, ethyl acetate, butanone, Folin-Ciocalteu reagent and HPTLC plates silica gel 60 F₂₅₄ were obtained from Merck, Darmstadt, Germany. Thiazol blue tetrazolium bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, MTT) were purchased from Carl Roth, Karlsruhe, Germany. Bidistilled water was prepared with a Destamat Bi 18E, Heraeus, Hanau, Germany. formic acid (98%) was from J.T. Baker, Deventer, Netherlands.

Ristivojević P., Lekić N., Cvijetić I., Krstić Đ., Andrić F., Milojković-Opsenica D, & Morlock G.E. (2022). Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics. Molecules, 27(18), 6062.

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Bioactivity Screening of Natural Compounds

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Polyethylene glycol 400, 2-aminoethyldiphenylborinate (97%), Fast Blue Salt B, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH, 97%), butyrylcholinesterase (BChE, from equine serum, ≥140 U/mg) and acetylcholinesterase (AChE, from Electrophorus electricus Linnæus, ≥245 U/mg, 10 kU/vial), tyrosinase (from mushroom, ≥1000 U/mg, 25 kU/vial), and solvents/reagents of analytical grade were purchased from Fluka Sigma-Aldrich, Schnelldorf, Germany. Luminescent marine A. fischeri bacteria (NRRL-B11177, DSM no. 5171) were obtained from the German Collection of Microorganisms and Cell Cultures. (2S)-2-Amino-3-(3,4-dihydroxyphenyl) propionic acid (levodopa) was obtained from Santa Cruz Biotechnology, Dallas, TX, United States. Ethanol, and methanol and its MS-grade were provided from Fisher Scientific, Schwerte, Germany. Formic acid (96%), toluene, dichloromethane, ethyl acetate, and HPTLC plates silica gel 60 F₂₅₄ were obtained by Merck, Darmstadt, Germany. Bidistilled water was prepared with a Destamat Bi 18E, Heraeus, Hanau, Germany.

Krstić Đ., Ristivojević P., Andrić F., Milojković-Opsenica D, & Morlock G.E. (2022). Quality Assessment of Apple and Grape Juices from Serbian and German Markets by Planar Chromatography—Chemometrics. Molecules, 27(12), 3933.

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Antioxidant Evaluation of Tinospora cordifolia

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HPLC grade reference standards of cordifolioside, magnoflorine, berberine chloride, gallic acid and rutin were purchased from Sigma-Aldrich Co. St.Louis, USA. Analytical grade chemicals; 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and trolox were procured from Sigma-Aldrich Co. St.Louis, USA. Folin–ciocalteu reagent and other chemicals used for analysis were of analytical grade, which were acquired from Merck, Mumbai, India. HPTLC plates, Silica gel 60 F₂₅₄ were purchased from Merck KGaA, Darmstadt, Germany. T. cordifolia plant powder sample was procured from the local market with commercial suppliers in Dharwad, Karnataka, India.

Manne M., Goudar G., Varikasuvu S.R., Khetagoudar M.C., Kanipakam H., Natarajan P., Ummiti M.D., Yenagi V.A., Chinthakindi S., Dharani P., Thota D.S., Patil S, & Patil V. (2021). Cordifolioside: potent inhibitor against Mpro of SARS-CoV-2 and immunomodulatory through human TGF-β and TNF-α. 3 Biotech, 11(3), 136.

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Analysis of Coccidiostats in Animal Feeds

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Coccidiostats: maduramycin ammonium, narasin, salinomycin sodium salt, monensin sodium salt, lasalocid A sodium salt, robenidine hydrochloride, dinitrocarbanilide (marker for nicarbazin) and nigericin sodium salt were purchased from Sigma-Aldrich (St. Louis, MO, USA). The feed samples were kindly provided by the National Feed Laboratory in Lublin. Anhydrous sodium sulphate and magnesium sulphate were purchased from Alfa Aesar (Thermo Fischer, GmbH, Germany). 500 mg SPE columns (6 ml volume, Bakerbond spe WP-CBX) were provided by Avantor (Gliwice, Poland). HPTLC plates Silica gel 60 F254 and 98% formic acid solution were supplied by Merck (Darmstadt, Germany). Acetonitrile and methanol (both MS purity) were purchased from Biosolve Chemie (Dieuze, France), while for SFPE experiments: acetonitrile, methanol and toluene (all MS purity) were provided by POCH (Gliwice, Poland).

Rybicki M.J., Becue I., Daeseleire E., Klimek-Turek A, & Dzido T.H. (2022). Solvent Front Position Extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by LC–MS/MS. Scientific Reports, 12, 3786.

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HPTLC Analysis of Antioxidant Capacity

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HPTLC plates silica gel 60 F 254 was purchased from Merck Ltd. (Mumbai, India). 1,1-diphenyl-2picrylhydrazyl (DPPH) and intestinal acetone powders from rats were purchased from Sigma Aldrich (Mumbai, India). Amylase HR reagent was obtained from Pro Lab Marketing Pvt. Ltd. (New Delhi, India). All other chemicals used in this study are of analytical grade.

Hiếu H.V., Tatipamula V.B., Killari K.N., Koneru S.T., & Nallapaty S. (2020). HPTLC Analysis, Antioxidant and Antidiabetic activities of Ethanol Extract of Moss Fissidens grandiflora. Indian Journal of Pharmaceutical Sciences, 82(3).

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