Cirazoline hydrochloride

Wire myography experiments were performed using 4-channel myograph systems (models 620M and 610M; Danish Myo Technology). Two different protocols were used. In the first protocol, we used tail arteries from 4 groups of mice; Cre-negative, Y/T KO, Cre-negative plus angiotensin II, and Y/T KO plus angiotensin II. The vessels were set to 5 mN preload tension and equilibrated for 40 minutes in Ca²⁺ containing HEPES-buffered Krebs solution at 37 °C (135.5 mmol/L NaCl, 5.9 mmol/L KCl, 1.2 mmol/L MgCl₂, 11.6 mmol/L HEPES, 11.5 mmol/L glucose, and 143.8 mmol/L Cl⁻). Vessels were then stimulated with 60 mmol/L KCl twice. Dose-response curves for AVP (arginine vasopressin; Tocris; #2935), 5-HT (5-hydroxytryptamine/serotonin; Sigma-Aldrich; #H4511-500MG), cirazoline hydrochloride (Tocris; #0888), and U 46619 thromboxane A₂ receptor agonist (Tocris; #1932) were generated. Force measurements were normalized to the length of the vessels and expressed as mN/mm. In the second protocol, abdominal aortae of vehicle and tamoxifen-treated Myh11-CreER^T2Yap1^fl/flWwtr1^fl/fl mice were mounted, and increments in circumference were done in HEPES-buffered Krebs solution at 37 °C. This was followed by a period of equilibration in nominally Ca²⁺-free solution where the passive force was recorded at the end.