Elecsys hbsag 2 quant 2

Manufactured by Roche

Sourced in Switzerland

The Elecsys HBsAg II quant II is a quantitative in vitro diagnostic test for the determination of hepatitis B surface antigen (HBsAg) in human serum and plasma samples. It is intended for use on Roche's Elecsys and cobas e immunoassay analyzers.

Automatically generated - may contain errors

Lab products found in correlation

Cobas e801, by Roche (2 mentions) Elecsys hbeag, by Roche (2 mentions) Lumipulse, by Fujirebio (1 mentions) M2000sp m2000rt realtime hbv test, by Abbott (1 mentions) Chemiluminescent microparticle immunoassay, by Abbott (1 mentions) Cobas ampliprep cobas taqman hbv test, by Roche (1 mentions) Cobas e602, by Roche (1 mentions)

7 protocols using elecsys hbsag 2 quant 2

Quantifying HBsAg and HBeAg in Cell-Based Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HBsAg concentration in cell lysates and cell culture supernatants was measured by electrochemiluminescence immunoassay (ECLIA) using the Elecsys HBsAg II quant II on a Cobas e801 instrument (Roche, Mannheim, Germany), according to the instructions of the manufacturer. Results were expressed in IU/ml. The HBeAg concentration in cell lysates and culture supernatants was measured using the Elecsys HBeAg on a Cobas e801 instrument (Roche, Mannheim, Germany), according to the manufacturer’s recommendations. Results were expressed in Sample/Cut off value (S/CO).
The linearity dynamic range of the assay was validated making serial dilutions of serum samples with known HBeAg levels.
To rule out the possibility of cross-reactivity of the core protein and HBeAg, HuH-7 cells were transfected with a full-length HBV genome harboring the G1896A Precore mutation. Furthermore, the possible interference of the intracellular cell lysate in the specificity of the assay was evaluated by challenging serum samples with known HBeAg or qHBsAg levels diluted with non-transfected cell lysates.

Elizalde M.M., Tadey L., Mammana L., Quarleri J.F., Campos R.H, & Flichman D.M. (2021). Biological Characterization of Hepatitis B virus Genotypes: Their Role in Viral Replication and Antigen Expression. Frontiers in Microbiology, 12, 758613.

+ Open protocol

+ Expand

Quantifying HBV Viral Antigens Reliably

Check if the same lab product or an alternative is used in the 5 most similar protocols

HBsAg concentration in cell lysates and culture supernatants was measured by electrochemiluminescence immunoassay (ECLIA) using the Elecsys HBsAg II quant II on a Cobas e801 instrument (Roche, Mannheim, Germany). Results were expressed in IU/ml. HBeAg concentration in cell lysates and supernatants was measured using the Elecsys HBeAg (Roche, Mannheim, Germany), in accordance with the manufacturer’s instructions. Results were expressed in Sample/Cut off value (S/CO).
The linearity dynamic range of the assay was validated making serial dilutions of serum samples with known HBeAg levels. Furthermore, the possible interference of the intracellular cell lysate in the specificity of the assay was evaluated by challenging serum samples with known HBeAg or qHBsAg levels diluted with non-transfected cell lysates.

Elizalde M.M., Mojsiejczuk L., Speroni M., Bouzas B., Tadey L., Mammana L., Campos R.H, & Flichman D.M. (2022). Molecular and biological characterization of hepatitis B virus subgenotype F1b clusters: Unraveling its role in hepatocarcinogenesis. Frontiers in Microbiology, 13, 946703.

+ Open protocol

+ Expand

Quantifying HBV Biomarkers in Hepatitis

Check if the same lab product or an alternative is used in the 5 most similar protocols

HBV DNA was measured by polymerase chain reaction with a limit of quantification of 10 IU/mL (ABBOTT RealTime HBV m2000^®, Abbott Molecular Inc., IL, United States). Serum HBsAg was quantified by Electro-chemiluminescence immunoassay Elecsys^®HBsAgII QuantII (Roche Diagnostic, Rotkreuz, Switzerland) according to the manufacturer’s instructions. The assay ranged from 0.05 to 117000 IU/mL. In highly concentrated samples above the upper limit, the value of manual dilution was multiplied by the dilution factor. Serum HBcrAg was measured using a quantitative fully automated chemiluminiscent enzyme immunoassay (LUMIPULSE^®, Fujirebio Europe, Belgium).
The monoclonal antibodies used in this two-step immunoassay measure simultaneously denatured HBeAg, HBV core antigen and the precore protein p22cr (aa-28 to aa-150). Samples were processed according to the manufacturer’s instructions. The lower limit of detection was 2.0 log U/mL, and a linear range of 3.0 log U/mL-7.0 log U/mL (1 kU/mL was equal to 3 log U/mL).

Broquetas T., Garcia-Retortillo M., Micó M., Canillas L., Puigvehí M., Cañete N., Coll S., Viu A., Hernandez J.J., Bessa X, & Carrión J.A. (2020). Hepatitis B surface antigen and hepatitis B core-related antigen kinetics after adding pegylated-interferon to nucleos(t)ids analogues in hepatitis B e antigen-negative patients. World Journal of Hepatology, 12(11), 1076-1088.

+ Open protocol

+ Expand

HBV DNA and HBsAg Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

HBV DNA was quantified using the Abbott m2000sp/m2000rt Realtime HBV test (Abbott Laboratories) with a lower limit of quantification of 10 IU/mL. HBsAg was quantified using the Roche Diagnostics Elecsys® HBsAg II quant II as previously described.⁷³ (link)

Zerbato J.M., Avihingsanon A., Singh K.P., Zhao W., Deleage C., Rosen E., Cottrell M.L., Rhodes A., Dantanarayana A., Tumpach C., Tennakoon S., Crane M., Price D.J., Braat S., Mason H., Roche M., Kashuba A.D., Revill P.A., Audsley J, & Lewin S.R. (2022). HIV DNA persists in hepatocytes in people with HIV-hepatitis B co-infection on antiretroviral therapy. eBioMedicine, 87, 104391.

+ Open protocol

+ Expand

Quantifying Hepatitis B Viral Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The culture supernatants and cell lysates of the pHBV1.3C-transfected cells were separately obtained from 12-well plates at the indicated time points. Subsequently, the extracellular and intracellular levels of HBsAg and HBeAg were assessed using electrochemiluminescence (ECL) assays with Elecsys HBsAg II quant II (the lower limit of detection is 0.05 IU/mL) (Roche, Mannheim, Germany) and Elecsys HBeAg (the cutoff ≥1.0 is positive) (Roche, Mannheim, Germany) in accordance with the manufacturer’s directions. These experiments were performed at least in triplicate.

Liao F., Xie J., Du R., Gao W., Lan L., Wang M., Rong X., Fu Y, & Wang H. (2023). Replication and Expression of the Consensus Genome of Hepatitis B Virus Genotype C from the Chinese Population. Viruses, 15(12), 2302.

+ Open protocol

+ Expand

Quantifying HBV Biomarkers in Patients

Check if the same lab product or an alternative is used in the 5 most similar protocols

HBeAg and anti-HBeAg were tested by chemiluminescent microparticle immunoassay (Abbott, IL). HBsAg was quantified by Elecsys® HBsAg II quant II (Roche Diagnostics, Switzerland). HBV DNA was measured by COBAS® AmpliPrep/COBAS® TaqMan® HBV Test, v2.0 (Roche Diagnostics, Switzerland).

Peña-Asensio J., Calvo-Sánchez H., Miquel J., Sanz-de-Villalobos E., González-Praetorius A., Torralba M, & Larrubia J.R. (2023). IL-15 boosts activated HBV core-specific CD8+ progenitor cells via metabolic rebalancing in persistent HBV infection. iScience, 27(1), 108666.

+ Open protocol

+ Expand

Comprehensive HBV Biomarker Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood samples were collected from all enrolled patients during the screening period. Samples were then continuously collected after enrollment every 24 weeks (the NAs group) or every 12 weeks (the Add-on group). Serological and biochemical markers of HBV were routinely tested in the central clinical laboratory. Serum HBV DNA levels were determined by the COBAS AmpliPrep/COBAS TaqMan HBV Test (Roche Molecular Systems, Inc, Branchburg, USA). The lower limit for HBV DNA detection was 20 IU/ml. Serum HBsAg levels were quantified by Elecsys HBsAg II quant II (Roche Diagnostics GmbH, Mannheim, Germany). The lower limit for HBsAg detection was 0.05 IU/ml. COBAS e602 (Roche Diagnostics GmbH, Mannheim, Germany) was used to detect HBsAb, HBeAg, and HBeAb levels. Serum levels of IFNG, IL1B, IL1RN, IL2, IL4, IL6, IL10, IL12A, IL17A, CCL2, CCL3, CCL5, CXCL8, CXCL10, TNF, and CSF2 were determined by flow-cytometer using AIMPLEX kit (Aimplex Biosciences, Inc., Beijing, China) according to the manufacturer’s instructions.

Wang W.X., Jia R., Jin X.Y., Li X., Zhou S.N., Zhang X.N., Zhou C.B., Wang F.S, & Fu J. (2023). Serum cytokine change profile associated with HBsAg loss during combination therapy with PEG-IFN-α in NAs-suppressed chronic hepatitis B patients. Frontiers in Immunology, 14, 1121778.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!