Western blot studies were performed as previously described6 (link). After the LPS and LECT2 stimulation, treated cells were collected and lysed in 1 × RIPA lysis buffer (Upstate Biotechnology) with a Complete Mini EDTA-free cocktail tablet (Roche Diagnostics) and PhosSTOP phosphatase inhibitor cocktail tablets (Roche Diagnostics). Protein samples were subjected to SDS-PAGE and transferred to polyvinylidene fluoride membranes, using an iBlot gel transfer system (Invitrogen). Membranes were blocked and then they were probed with antibodies for 16 h (Phospho-SAPK/JNK (Thr183/Tyr185) Antibody (Cell Signaling, Danvers, MA), SAPK/JNK Antibody (Cell Signaling, Danvers, MA), Phospho-p38 MAPK (Thr180/Tyr182) Antibody (Cell Signaling, Danvers, MA), p38 MAPK Antibody (Cell Signaling, Danvers, MA), Phospho-p44/42 MAPK(Erk1/2) (Thr202/Tyr204) Antibody (Cell Signaling, Danvers, MA), p44/42 MAPK(Erk1/2) Antibody (Cell Signaling, Danvers, MA) Phospho-SEK1/MKK4 (Ser257) Antibody (Cell Signaling, Danvers, MA), SEK1/MKK4 Antibody (Cell Signaling, Danvers, MA) Afterward, membranes were washed and then incubated with anti-rabbit IgG horseradish peroxidase-linked antibody (Cell Signaling) for 1 h. Protein bands were visualized with ECL Prime Western blotting detection reagent (GE Healthcare UK Ltd.).
Phospho sapk jnk thr183 tyr185 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Phospho-SAPK/JNK (Thr183/Tyr185) Antibody is a primary antibody that specifically recognizes the phosphorylated form of SAPK/JNK (Stress-Activated Protein Kinase/c-Jun N-Terminal Kinase) at Thr183 and Tyr185. This antibody is designed for use in Western blotting applications to detect the activation of SAPK/JNK, which is a key signaling pathway involved in cellular stress response and other cellular processes.
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Lab products found in correlation
Sapk jnk antibody, by Cell Signaling Technology (2 mentions)
Iblot gel transfer system, by Thermo Fisher Scientific (1 mentions)
P38 mapk antibody, by Cell Signaling Technology (1 mentions)
P44 42 mapk erk1 2 antibody, by Cell Signaling Technology (1 mentions)
Phospho p44 42 mapk erk1 2 thr202 tyr204 antibody, by Cell Signaling Technology (1 mentions)
Complete mini edta free cocktail tablet, by Roche (1 mentions)
Phospho p38 mapk thr180 tyr182 antibody, by Cell Signaling Technology (1 mentions)
Anti rabbit igg horseradish peroxidase linked antibody, by Cell Signaling Technology (1 mentions)
Phosstop phosphatase inhibitor cocktail tablet, by Roche (1 mentions)
Ecl prime western blotting detection reagent, by GE Healthcare (1 mentions)
Dox hydrochloride, by Merck Group (1 mentions)
α sma antibody, by Merck Group (1 mentions)
Sqstm1 p62 antibody, by Cell Signaling Technology (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody, by Cell Signaling Technology (1 mentions)
Chloroquine, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 goat anti rabbit immunoglobulin g, by Thermo Fisher Scientific (1 mentions)
Sp600125, by Merck Group (1 mentions)
Alexa fluor 488 goat anti mouse immunoglobulin g igg, by Thermo Fisher Scientific (1 mentions)
Lc3b antibody, by Cell Signaling Technology (1 mentions)
2 protocols using phospho sapk jnk thr183 tyr185 antibody
1
Western Blot Analysis of MAPK Signaling
2
Molecular Signaling Pathway Analysis
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Galectin‐3 antibody, SAPK/JNK antibody, phospho‐SAPK/JNK (Thr183/Tyr185) antibody, Phospho‐PI3 Kinase Class III (Ser249) antibody, PI3 Kinase Class III antibody, SQSTM1/p62 antibody, LC3B antibody, and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) antibody were purchased from Cell Signaling (Danvers, MA, USA). Collagen type 1A1 (COL1A1) antibody and interleukin (IL)‐1R‐associated kinase 1 (IRAK‐1) were purchased from Santa Cruz (Dallas, TX, USA). The α‐SMA antibody was purchased from Sigma‐Aldrich (St. Louis, MO, USA). Alexa Fluor 488 goat anti‐mouse immunoglobulin G (IgG) and Alexa Fluor 594 goat anti‐rabbit immunoglobulin G were purchased from Invitrogen (Carlsbad, CA, USA). DOX hydrochloride and SP600125 were purchased from Sigma‐Aldrich. Chloroquine was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Pioglitazone hydrochloride was purchased from Fujifilm (Tokyo, Japan). ODN2088 and GW1929 were purchased from AdipoGen Life Sciences (San Diego, CA, USA).
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