Inverted light microscope was applied to observe the structure of HIPEs and oil droplets’ distribution according to Chen et al. [29 (link)]. The samples were placed in glass slides, covered with cover glass and compacted gently, and the microstructure was then observed by a Revolve Hybrid Microscope (Echo, San Diego, CA, USA) and recorded using a retina screen iPad Pro tablet (Apple Inc., Cupertino, CA, USA) attached to the microscope. The magnification was 20 times.
Revolve hybrid microscope
Manufactured by Echo Medical Systems
Sourced in United States
The Revolve Hybrid Microscope is a versatile laboratory equipment designed for multiple imaging techniques. It combines optical and digital capabilities to provide high-quality imaging solutions. The core function of the Revolve Hybrid Microscope is to enable users to capture and analyze various biological and material samples through a range of magnification levels and imaging modalities.
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Lab products found in correlation
3 protocols using revolve hybrid microscope
1
Microstructural Analysis of HIPEs
2
Subcellular Localization of eGFP-tagged esilIL-16
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Immunocytochemical staining was used to examine the subcellular location of the N-terminal EsIL-16 prodomain in HEK293T cells. Briefly, pretreated cells were blocked with 3% bovine serum albumin (BSA) for 30 min at 37 °C and then incubated with antibodies recognizing GFP-tagged EsIL-16 overnight at 4 °C. After washing with PBS, the cells were incubated with 3% BSA for 10 min, after which the goat antimouse Alexa Fluor 488 secondary antibody (1:1000 dilution in 3% BSA) was added. The reaction was performed in the dark for 1 h at 37 °C, and then the cells were washed with PBS. Finally, the cells were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (AnaSpec) for 10 min at room temperature, washed, and observed under a Revolve Hybrid Microscope (Echo).
3
Myxoma Virus Infection Kinetics
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Myxoma virus infectivity was assessed over a 24 h period. Cells were handled exactly as in the cell culture and Myxoma virus infection section. Cells were imaged on a Revolve hybrid microscope obtained from ECHO (CA, US). Images were captured at 0 h, 6 h, 12 h, and 24 h using a FITC and transfluorescent module. Cell counts were performed on ImageJ (NIH, MD, US) to determine the number of GFP positive cells and the number of total cells present in each respective image. Cell numbers were used to calculate percent infectivity at each time point.
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