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G csf

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G-CSF is a type of lab equipment used in medical research and clinical settings. It is used to produce a specific protein that stimulates the growth and development of white blood cells. The core function of G-CSF is to assist in the production and maturation of certain types of white blood cells, which play a crucial role in the body's immune response.

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Lab products found in correlation

Gm csf, by Fujifilm (2 mentions) Stemspan sfem, by STEMCELL (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Anti mouse igg hrp, by GE Healthcare (1 mentions) Hrp conjugated anti actin antibody, by Santa Cruz Biotechnology (1 mentions) Ifn γ, by Fujifilm (1 mentions) Il 10, by Fujifilm (1 mentions) Flt3l, by Fujifilm (1 mentions) Stemfit ak03 medium, by Ajinomoto (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) Ficoll paque, by GE Healthcare (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Complete rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)

5 protocols using g csf

1

Immunophenotyping and Phosphorylation Assay

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Primary antibodies included FITC- or PE-conjugated anti-human CD11b, PE-conjugated anti-human CD16 (Miltenyi Biotec K.K., Tokyo, Japan); FITC-rat IgG (isotype control) (abcam, Cambridge, UK); anti-human CD11b monoclonal (BioLegend, San Diego, CA); anti-human phospho-STAT5 (Millipore, Billerica, MA). Secondary detection antibody (anti-rat IgG-HRP(HAF005)) was from R&D systems (Minneapolis, MN) and anti-mouse IgG-HRP was from GE Healthcare (Little Challfont Buckinghamshire, UK). HRP-conjugated anti-actin antibody was from Santa Cruz Biotechnology (Dallas, TX). E. coli-derived recombinant human GM-CSF was from ATGen (Seongnam, South Korea). Recombinant cytokines, and other proteins included G-CSF, C5a, Interleukin (IL)-6, IL-8, IL-10, Interferon (IFN)-β, IFN-γ (all from Wako, Osaka, Japan).
Kusakabe Y., Uchida K., Hiruma T., Suzuki Y., Totsu T., Suzuki T., Carey B.C., Yamada Y, & Trapnell B.C. (2014). A standardized blood test for the routine clinical diagnosis of impaired GM-CSF signaling using flow cytometry. Journal of immunological methods, 413, 1-11.
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2

Generation of iPSCs from Peripheral Blood

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Mononuclear cells (MNCs) were isolated freshly from the peripheral blood of the AD patient and normal subject using the Ficoll-Paque™ PLUS method (GE Healthcare, USA). Isolated peripheral-derived MNCs (PBMCs) were cultured for 4 days in MNC media containing 50 ng/ml interleukin-6 (IL-6), 50 ng/ml stem cell factor (SCF), 10 ng/ml thrombopoietin (TPO), 20 ng/ml Flt3 ligand (Flt-3L), 20 ng/ml interleukin-3 (IL-3), and 10 ng/ml granulocyte colony-stimulating factor (G-CSF) (all from WAKO, Japan) in StemFit AK03 medium (kindly provided by Ajinomoto, Japan). The MNCs were then infected with SeVdp (KOSM) 302L at MOI of 3~10 [8 (link)] and transferred into 6-well dishes coated with iMatrix-511 (Matrixome, Japan). From the next day, 500 ul of StemFit AK03 medium was added every day for 4 days, after which the medium was fully changed every other day until iPSC-like colonies emerged. Sub-culturing and expansion of the cells was then undertaken until the generated iPSCs became stable for characterization and storage, normally at passage 10.
Li L., Roh J.H., Chang E.H., Lee Y., Lee S., Kim M., Koh W., Chang J.W., Kim H.J., Nakanishi M., Barker R.A., Na D.L, & Song J. (2018). iPSC Modeling of Presenilin1 Mutation in Alzheimer's Disease with Cerebellar Ataxia. Experimental Neurobiology, 27(5), 350-364.
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3

Isolation and Culture of AML Cells

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Samples were prepared from peripheral blood collected from newly diagnosed and untreated AML patients. Mononuclear cells were isolated by Ficoll-Paque (GE Healthcare Life Sciences) density gradient centrifugation. Cells were cultured in the serum-free medium StemSpan SFEM (referred to as SFEM) purchased from Stem Cell Technologies and supplemented with recombinant human stem cell factor (SCF, 100 ng/ml), interleukin-3 (IL-3, 20 ng/ml), FMS-like tyrosine kinase ligand (FLT3L, 100 ng/ml), granulocyte colony-stimulating factor (G-CSF, 20 ng/ml) and granulocyte-macrophage colony-stimulating factor (GM-CSF, 20 ng/ml) (Shenandoah Biotechnology). All cultures were incubated at 37°C with 5% CO2. Informed consent was obtained from all patients under Penn State College of Medicine Institutional Review Board-approved protocol according to the Declaration of Helsinki.
Pearson J.M., Tan S.F., Sharma A., Annageldiyev C., Fox T.E., Abad J.L., Fabrias G., Desai D., Amin S., Wang H.G., Cabot M.C., Claxton D.F., Kester M., Feith D.J, & Loughran TP J.r. (2019). Ceramide analog SACLAC modulates sphingolipid levels and Mcl-1 splicing to induce apoptosis in acute myeloid leukemia. Molecular cancer research : MCR, 18(3), 352-363.
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4

Cell Culture Protocol for Hematopoietic and Solid Tumor Cell Lines

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HEK 293T, U937, K562 and THP-1 cells were obtained from the American Type Culture Collection (ATCC) and the German Collection of Microorganisms and Cell Cultures (DSMZ). De-identified patient samples were provided by Loma Linda University (Loma Linda, CA, USA) and collaborators at the Penn State Cancer Institute in compliance with institutional review board regulations. Cells were cultured with RPMI 1640 plus (Mediatech, Manassas, VA, USA) with a 10% heat-inactivated Fetal bovine serum (FBS) (HyClone, Rockford, IL, USA) and 1% penicillin-streptomycin. HEK-293T cells were cultured in DMEM (CellGro) supplemented with 10% FBS. Human AML primary cells (AML-1), previously expanded in mice, were cultured in StemSpan SFEM (Stem Cell Technologies, Cambridge, MA, USA), supplemented with a recombinant human stem cell factor (SCF, 100 ng/mL), IL3 (20 ng/mL), FMS-like tyrosine kinase ligand (FLT3L, 100 ng/mL), G-CSF (20 ng/mL), and GM-CSF (20 ng/mL; Shenandoah Biotechnology, Warwick, PA), as well as 1% penicillin-streptomycin.
Klink M., Rahman M.A., Song C., Dhanyamraju P.K., Ehudin M., Ding Y., Steffens S., Bhadauria P., Iyer S., Aliaga C., Desai D., Huang S., Claxton D., Sharma A, & Gowda C. (2021). Mechanistic Basis for In Vivo Therapeutic Efficacy of CK2 Inhibitor CX-4945 in Acute Myeloid Leukemia. Cancers, 13(5), 1127.
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5

Long-term G-CSF Exposure in Breast and Colon Cancer

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Mouse breast cancer cell (4T1) and colon cancer cell (CT26) were purchased from ATCC (Manassas, VA, USA) and cultured in RPMI-1640 complete medium containing 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). Both cell lines were cultivated with 5% at 37 °C CO2 in a humidified atmosphere and treated with G-CSF (Shenandoah Biotechnology, Warwick, PA) for either 10 days (short term) or 7 weeks (long term) at 25 ng/ml. Our previous study 20 (link) showed that the cytokine expressions after 6 weeks treatment of G-CSF were significantly different with short term groups. So we selected 7 weeks treatment in this study. Cells treated with PBS were used as the control group. Supernatants of long-term culture were saved for cytokine analysis and cell pellets were saved from RNA extraction.
Zhang W., Karagiannidis I., De Santana Van Vliet E., Yao R., Beswick E.J, & Zhou A. (2021). Granulocyte Colony-Stimulating Factor Promotes an Aggressive Phenotype of Colon and Breast Cancer Cells with Biochemical Changes Investigated by Single-Cell Raman Microspectroscopy and Machine Learning Analysis. The Analyst, 146(20), 6124-6131.
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